[Effects of salidroside on proliferation, apoptosis, phagocytosis, ROS and NO production of murine peritoneal macrophages in vitro].

AIM: To investigate the effects of salidroside(Sal) on proliferation, apoptosis, phagocytosis, the production of ROS and NO of murine peritoneal macrophages in vitro as well as its immunoregulation. METHODS: The single cell suspension of murine peritoneal macrophages was prepared under sterile condition, then co-cultured with different concentrations of Sal(80, 160 and 320 µmol/L)for 4 hours prior to stimulation with LPS and IFN-γ, the proliferation of macrophages was measured by MTT colorimetry. The effect of Sal on the apoptosis of Sytox® Green-labelled peritoneal macrophages induced by CHX was detected by Fluorescence enzyme-labelled meter. FCM was used to detect the effect of Sal on phagocytosis of peritoneal macrophages. Fluorescence enzyme-labelled meter was used to measure the effects of Sal on ROS of H(2);DCFDA-labelled macrophages induced by LPS and IFN-γ. Griess Gragent was used to detect the role of Sal in production of NO in peritoneal macrophages activated by LPS and IFN-γ. RESULTS: MTT result demonstrated that Sal could promote the proliferation of peritoneal macrophages activated by LPS and IFN-γ at the final concentrations of 80, 160, 320 µmol/L, respectively (P<0.05). The result of Fluorescence enzyme-labelled meter detected showed that Sal at the final concentration of 160 µmol/L could inhibit apoptosis of peritoneal macrophages induced by CHX(P<0.01). FCM analysis showed that different concentrations of Sal significantly promoted the phagocytosis of peritoneal macrophages which include un-activated and activated by LPS and IFN-γ(P<0.05). Fluorescence enzyme-labelled meter showed that Sal could reduce the production of ROS in activated peritoneal macrophages induced by LPS and IFN-γ(P<0.05). Sal also increased the production of NO in activated peritoneal macrophages induced by LPS and IFN-γ(P<0.05). CONCLUSION: Sal can promote proliferation of peritoneal macrophages stimulated by LPS and IFN-γ, and it can inhibit apoptosis of peritoneal macrophages induced by CHX, Sal also can promote the phagocytosis of peritoneal macrophages which include un-activated and activated by LPS and IFN-γ, Sal can reduce the production of ROS in activated peritoneal macrophages induced by LPS and IFN-γ, while Sal can promote the production of NO in activated peritoneal macrophages induced by LPS and IFN-γ.

[Effect of forsythia suspense extract on phagocytosis of peritoneal macrophages and NO production in vitro].

AIM: To investigate the effect of forsythia suspensa (FS) extract on phagocytosis of peritoneal macrophages and NO production in vitro. METHODS: The peritoneal macrophagess were isolated from BALB/c mice. After stained with CFDA-SE, the DH5alpha were co-cultured with peritoneal macrophagess for 3 h. The effect of FS extract on cyto-phagocytesis in vitro was analyzed by flow cytometry. The peritoneal macrophages were stimulated and activated by LPS in vitro. The effect of FS extract on NO production of the peritoneal macrophages in vitro was measured by NO assay kit. RESULTS: FCM analysis showed that FS extract significantly promoted the phagocytosis of peritoneal macrophages at the final concentration of 40, 80, 160 mg/L, respectively (P<0.05). It also decreased the production of NO at different concentration induced by LPS (P<0.05). CONCLUSION: FS extract can promote phagocytosis of peritoneal macrophages and inhibit NO production in vitro.

[Effects of Forsythia suspense extract on cell proliferation and activation of the mouse T lymphocytes in vitro].

AIM: To investigate the effect of Forsythia suspensa (FS) extract on the activation and proliferation of the mouse T lymphocytes in vitro as well as its immunosuppressive effect. METHODS: The lymphocytes were isolated from the lymphoid nodes of BALB/c mice. Fluorescence conjugated monoclonal antibodies and flow cytometry were used to detect the expression of CD69, CD25 and CD 71 of the activated T lymphocytes in vitro in response to Concanavalin A (ConA). Stained with CFDA-SE, the mouse lymphocytes were stimulated by ConA. The effect of FS extract on cell proliferation in vitro was analyzed by flow cytometry. RESULTS: FS extract decreased the expression of CD69, CD25 and CD71 at the final concentration of 40, 80 and 160 mg/L, respectively(P<0.05). CFDA-SE staining showed that FS extract at different concentration significantly inhibited the proliferation of lymphocytes induced by ConA (P<0.05). CONCLUSION: FS extract can inhibit the activation and proliferation of T lymphocytes in response to ConA.