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Cell Mol Neurobiol ; 32(7): 1089-97, 2012 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-22573254

RESUMO

It is well known that neural stem cells (NSC) could promote the repairment after spinal cord injury, but the underlying mechanism remains to be elucidated. This study showed that the transplantation of NSC significantly improved hindlimb locomotor functions in adult rats subjected to transection of the spinal cord. Biotin dextran amine tracing together with the stimulus experiment in motor sensory area showed that little CST regeneration existed and functional synaptic formation in the injury site. Immunocytochemistry and RT-PCR demonstrated the secretion of NGF, BDNF, and NT-3 by NSC in vitro and in vivo, respectively. However, only mRNA expression of BDNF and NT-3 but not NGF in injury segment following NSC transplantation was upregulated remarkably, while caspase-3, a crucial apoptosis gene, was downregulated simultaneously. These provided us a clue that the functional recovery was correlated with the regulation of BDNF, NT-3, and caspase-3 in spinal cord transected rats following NSC transplantation.


Assuntos
Fator Neurotrófico Derivado do Encéfalo/biossíntese , Células-Tronco Neurais/transplante , Neurotrofina 3/biossíntese , Recuperação de Função Fisiológica/fisiologia , Traumatismos da Medula Espinal/metabolismo , Transplante de Células-Tronco , Animais , Células Cultivadas , Regulação da Expressão Gênica , Sobrevivência de Enxerto/fisiologia , Fatores de Crescimento Neural/biossíntese , Regeneração Nervosa/fisiologia , Ratos , Ratos Sprague-Dawley , Traumatismos da Medula Espinal/cirurgia , Transplante de Células-Tronco/métodos , Regulação para Cima/fisiologia
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