RESUMO
OBJECTIVE: The incidence and progression of infantile hemangioma depend on several factors. The objective of the present study was to analyze the expression of MMP-9, VEGF, and p16 in hemangioma, and evaluate the clinical significance of the correlation between the gene and protein expression of MMP-9 and VEGF. PATIENTS AND METHODS: 60 pathological tissue samples from patients with hemangioma (30 cases of proliferative hemangioma and 30 cases of degenerative hemangioma) and 30 normal epidermal tissue samples were collected. Tissues were examined by immunohistochemistry. Additionally, MMP-9 and VEGF gene expression were evaluated by qRT-PCR to determine the correlation between gene and protein level. RESULTS: Immunohistochemistry showed that the expression of MMP-9 and VEGF was higher in proliferative hemangioma, while the expression of p16 was lower, and the differences were statistically significant (p<0.05). According to qRT-PCR, MMP-9 and VEGF gene expression were positively correlated with their protein expression (p<0.05). CONCLUSIONS: The tumor suppressor gene p16 is negatively correlated with the occurrence and degradation of hemangioma. Furthermore, the gene and protein expression of MMP-9 and VEGF in infantile hemangioma are positively correlated. The levels of MMP-9 and VEGF are higher in the proliferative stage of hemangioma than other stages. Both MMP-9 and VEGF facilitate angiogenesis and tumorigenesis, and are likely to serve as markers for the development of hemangioma in the future.
Assuntos
Inibidor p16 de Quinase Dependente de Ciclina/metabolismo , Hemangioma/patologia , Metaloproteinase 9 da Matriz/metabolismo , Fator A de Crescimento do Endotélio Vascular/metabolismo , Estudos de Casos e Controles , Feminino , Hemangioma/metabolismo , Humanos , Masculino , Metaloproteinase 9 da Matriz/genética , RNA/isolamento & purificação , RNA/metabolismo , Fator A de Crescimento do Endotélio Vascular/genéticaRESUMO
One case of arytenoids dislocation after removal of a foreign body from the trachea was reported. After operation, throat pain, hoarseness and laborious speech, arytenoids dislocation was revealed by electronic laryngoscopy.
Assuntos
Cartilagem Aritenoide/lesões , Corpos Estranhos/cirurgia , Traqueia/lesões , Rouquidão , Humanos , LaringoscopiaRESUMO
Ten cases of endomyocardial fibrosis (EMF) were diagnosed with angiocardiography in showing a huge right atrium' tricuspid regurgitation and obliteration of right ventricular apex. The diagnosis was confirmed in 9 of them pathologically after right ventricular endomyocardial biopsy (EMB) in showing striking thickening of endocardium with presence of fibrosis. The remaining case was not confirmed with EMB. It is concluded that EMB is a quite useful method in the diagnosis of EMF. The cause of misdiagnosis in the tenth case was discussed.
