[Changes in diversity of marsh plant communities under shrub encroachment in Sanjiang Plain and their soil control factors]. / çŒæœ¨æ‰©å¼ åŽ‹åŠ›ä¸‹ä¸‰æ±Ÿå¹³åŽŸæ²¼æ³½æ¤ç‰©ç¾¤è½å¤šæ ·æ€§å˜åŒ–åŠå ¶åœŸå£¤æŽ§åˆ¶å› å­.

In this study, we explored the changes in plant community diversity and their relationship with soil factors under shrub encroachment pressure by selecting four marsh areas in Sanjiang Plain with different degrees of shrub cover (a, 0≤a≤100%), including marsh with no shrub encroachment (a=0), light shrub encroachment (0

Research on Chemical Mechanical Polishing Technology for Zirconium-Based Amorphous Alloys.

Crystallization often occurs in the processing of amorphous alloys, causing the materials lose their excellent properties. The study adopts chemical mechanical polishing of amorphous alloys, presenting the effect of the rotational speed of the polishing turntable, size of abrasive, polishing pressure, and oxidant concentration. The Taguchi method is used to find the best processing parameters, and AFM is used to characterize the machined material surface. At the same time, XPS is used to detect the change of oxide film composition with the addition of oxidant. The results indicate the optimum process parameters: rotational speed of the polishing turntable is 75 r/min, polishing pressure is 28.3 kPa, the size of abrasive is 0.5 µm, and the size of abrasive is a significant factor affecting surface roughness Sa. In addition, as the size of abrasive increases, the material removal rate increases while the surface roughness Sa increases. At pH 10, with an abrasive particle size of 0.5 µm, as the H2O2 concentration increases, the MRR first rapidly decreases at 0.21 wt.% H2O2, and then gradually increases, while the Sa decreases. Furthermore, with the addition of oxidant, the main composition of the surface oxide film changes from oxide to hydroxide, and the contents of Zr4+ and Cu0/Cu1+ elements increase. The findings can provide a feasible chemical mechanical polishing process for zirconium-based amorphous alloys to obtain a satisfactory polishing effect.

Spatial Resolution Limit for Nanoindentation Mapping on Metallic Glasses.

Spatial heterogeneity, as a crucial structural feature, has been intensively studied in metallic glasses (MGs) using various techniques, including two-dimensional nanoindentation mapping. However, the limiting spatial resolution of nanoindentation mapping on MGs remains unexplored. In this study, a comprehensive study on four representative MGs using nanoindentation mapping with a Berkovich indenter was carried out by considering the influence of a normalized indentation spacing d/h (indentation spacing/maximum indentation depth). It appeared to have no significant correlation with the measured hardness and elastic modulus when d/h > 10. The hardness and elastic modulus started to increase slightly (up to ~5%) when d/h < 10 and further started to decrease obviously when d/h < 5. The mechanism behind these phenomena was discussed based on a morphology analysis of residual indents using scanning electron microscopy and atomic force microscopy. It was found that the highest spatial resolution of ~200 nm could be achieved with d/h = 10 using a typical Berkovich indenter for nanoindentation mapping on MGs, which was roughly ten times the curvature radius of the Berkovich indenter tip (not an ideal triangular pyramid) used in this study. These results help to promote the heterogeneity studies of MGs using nanoindentation that are capable of covering a wide range of length scales with reliable and consistent results.

Estimating unconfirmed COVID-19 infection cases and multiple waves of pandemic progression with consideration of testing capacity and non-pharmaceutical interventions: A dynamic spreading model.

The novel coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has unique epidemiological characteristics that include presymptomatic and asymptomatic infections, resulting in a large proportion of infected cases being unconfirmed, including patients with clinical symptoms who have not been identified by screening. These unconfirmed infected individuals move and spread the virus freely, presenting difficult challenges to the control of the pandemic. To reveal the actual pandemic situation in a given region, a simple dynamic susceptible-unconfirmed-confirmed-removed (D-SUCR) model is developed taking into account the influence of unconfirmed cases, the testing capacity, the multiple waves of the pandemic, and the use of non-pharmaceutical interventions. Using this model, the total numbers of infected cases in 51 regions of the USA and 116 countries worldwide are estimated, and the results indicate that only about 40% of the true number of infections have been confirmed. In addition, it is found that if local authorities could enhance their testing capacities and implement a timely strict quarantine strategy after identifying the first infection case, the total number of infected cases could be reduced by more than 90%. Delay in implementing quarantine measures would drastically reduce their effectiveness.

Aerobic composting of chicken manure with penicillin G: Community classification and quorum sensing mediating its contribution to humification.

Chicken manure containing antibiotics is a hazardous biological waste. The purpose of our study was to investigate how different concentrations of penicillin G alter the bacterial community to affect humification during aerobic composting of chicken manure. The effect of quorum sensing on the bacterial community was also evaluated. Penicillin G mainly affects low fold changes (within 4) for low-abundance (within 200) microbial genera. We found that the bacterial community cooperated to regulate humus and humic acid synthesis. The microbial genera that make up the bacterial community are different, but each bacterial community may have the same ecological function. Quorum sensing affects humic acid synthesis by regulating carbohydrate metabolism and amino acid metabolism in bacterial communities through mechanisms such as the pentose phosphate pathway and the shikimate pathway. This work presents an understanding of the impact of quorum sensing on the collaboration between bacterial communities during composting.

Driving factors of nitrogen conversion during chicken manure aerobic composting under penicillin G residue: Quorum sensing and its signaling molecules.

This study explored effects of different concentrations of penicillin G on nitrogen conversion, bacterial community composition, and quorum sensing during chicken manure aerobic composting. After composting, adding penicillin G down-regulated the abundance of 71 genera and up-regulated the abundance of 103 genera. These bacterial genera were mainly Firmicutes and Proteobacteria. 16S rRNA gene sequencing was employed for function prediction, and the results showed that the addition of penicillin G increased nitrification, reduced denitrification. The autoinducer-1 (AI-1), autoinducer-3 (AI-3) and Phr signal molecules further participated in the nitrogen cycle by regulating the population behavior among multiple bacterial genera. In addition, SEM analysis showed that the quorum sensing system negatively regulated the abundance of genus related to the nitrogen conversion during chicken manure aerobic composting. This is a new theoretical analysis of the research on the treatment of hazardous materials.

Intraspecific and interspecific quorum sensing of bacterial community affects the fate of antibiotic resistance genes during chicken manure composting under penicillin G stress.

In this study, the effects of penicillin G (PENG) on the fate of bacterial communities and ß-lactamase antibiotic resistance genes (ARGs) during chicken manure composting were assessed, to illustrate the roles of PENG in ARGs behavior. The results showed that the total absolute abundances of 9 ARGs and 4 mobile genetic elements (MGEs) was significantly increased by PENG (P < 0.05). Dozens of potential hosts for ARGs were predominantly affiliated with Firmicutes, Proteobacteria, and Actinobacteria. Meanwhile, the higher concentration of PENG significantly increased the abundance of luxI and luxS in quorum sensing (QS) (P < 0.05), which enhanced the frequency of inter/intraspecific gene "communication." Redundancy analysis and structural equation modeling further revealed that QS had a strong regulatory role in horizontal gene transfer of ARGs mediated via MGEs. These results provide new insight into the mechanism of ARGs propagation in aerobic composting modified by PENG.

Links between microbial compositions and metabolites during aerobic composting under amoxicillin stress was evaluated by 16S rRNA sequencing and gas chromatography-mass spectrometry: Benefit for the plant growth.

The purpose of this study was to identify the relationship between core bacteria and metabolites during aerobic composting and analyze the effects of metabolites on plant growth. The results revealed that amoxicillin might affect the generation and transformation of metabolites by reconstructs the bacterial communities. The peak area ratios (PAR) of esters and fatty acids (FAs) were increased, while sterols decreased during composting. Furthermore, the correlation analysis showed that the production of FAs, esters and sterols is strongly correlated with Oceanobacillus, Corynebacterium, Psychrobacter, Xanthomonadaceae, Pusillimonas and Gracilibacillus. Moreover, 36 key metabolites were screened out, the PAR of the propanoic acid ethyl ester and oleic acid that benefit plant growth were increased in amoxicillin groups. However, the PAR of environmental pollutants, such as n-hexadecanoic acid and 3ß, 5ß-Cholestan-3-ol is the opposite. Therefore, composting can eliminate the environmental risks caused by antibiotic residues in feces and promote plant growth.

Evaluation of humic acid conversion during composting under amoxicillin stress: Emphasizes the driving role of core microbial communities.

This study aimed to investigate the effects of various amoxicillin (AMX) concentrations on humic acid (HA) formation and the bacterial community structure (BCS) during chicken manure aerobic composting. The findings showed that AMX caused a rise in pH and a shortening of the thermophilic cycle during the thermophilic period. Moreover, a high concentration of AMX (150 mg/kg) inhibited community succession and humification at maturity. In addition to influencing environmental factors, AMX increased the relative abundance (RA) of the dominant bacteria in the thermophilic phase (Firmicutes) and decreased the RA of the dominant bacteria in the mature phase (Proteobacteria). From carbon conversion, AMX promoted fulvic acid (FA) anabolism in the early period and inhibited HA accumulation in the later period. As a result, the primary impact of AMX on HA transformation was the alteration of the BCS, ultimately affecting the quality of compost.

Evaluation of nitrogen conversion pathway during composting under amoxicillin stress: Mainly driven by core microbial community.

The aim of this study was to explore the effects of different concentrations of amoxicillin (AMX) on nitrogen (N) conversion and bacterial community structure during aerobic composting. The results revealed that AMX led to a lower temperature and increased pH during the thermophilic phase of composting. AMX inhibited the relative abundance (RA) of Firmicutes at the initial phase but increased the RA of Actinobacteria and Bacteroidetes compared with the control treatment. The core bacterial community linked to N conversion was determined by network analysis. AMX decreased the RA of amoA, a gene related to nitrification, and increased the RAs of nirK and nosZ, which are related to denitrification. Meanwhile, AMX inhibited the activity of ammonia-oxidizing bacteria but promoted the activity of denitrifying bacteria. Therefore, the main adverse effect of AMX on compost quality is to change the microbial community structure and affect the physical and chemical properties of composting.