RESUMO
PURPOSE: To synthesize and characterize a magnetic micromolecular delivery system based on PVP hydrogel with polyvinyl alcohol (PVA) as the crosslinker. METHODS: The microparticles were successfully prepared using 25 kGy Co-60 gamma-ray irradiation and characterized. Biocompatibility, in vitro and in vivo drug release tests were carried out. RESULTS AND DISCUSSION: Bleomycin was quantitatively released with in slightly over 8 h (hours) from the nanospheres containing 1mg bleomycin while the time was longer for those containing 5 mg. On the other hand free bleomycin quantitatively passed through the dialysis baffle with in only 3.5 h. For both 5 and 1 mg of bound bleomycin, it took up to 2 h to reach peak concentration compared to 30 min for the free drug. CONCLUSION: The PVP hydrogel magnetic nanospheres exhibited passive drug release that could be exploited to enhance therapeutic efficacy. The present results indicate that PVP hydrogel based magnetic nanospheres have potential as drug carriers in magnetic guided chemotherapeutic drug delivery.
Assuntos
Antibióticos Antineoplásicos/administração & dosagem , Bleomicina/administração & dosagem , Álcool de Polivinil/química , Animais , Antibióticos Antineoplásicos/química , Antibióticos Antineoplásicos/farmacocinética , Materiais Biocompatíveis , Bleomicina/química , Bleomicina/farmacocinética , Cromatografia Líquida de Alta Pressão , Feminino , Raios gama , Hemólise/efeitos dos fármacos , Magnetismo , Masculino , Teste de Materiais , Camundongos , Camundongos Endogâmicos BALB C , Microscopia Eletrônica de Transmissão e Varredura , Nanopartículas , Povidona/química , Coelhos , Padrões de Referência , Sais de Tetrazólio , Tiazóis , Distribuição TecidualRESUMO
Objective To explore the expression of HIF-2? and VEGF in renal cell carcinoma (RCC) and their correlation with angiogenesis and metastasis of renal cell carcinoma. Methods Expression of HIF-2? and VEGF were assessed by immunohistochemical straining in 48 cases of renal cell carcinomas. Results In 48 cases of renal cell carcinomas, positive rate of HIF-2? was 70.8%. Strong expression of HIF-2? was observed in 12 cases, moderate in 10 cases, weak in 12 cases and no expression in 14cases.There was a significant difference in HIF-2? expression between carcinoma tissues and control tissues (P
