Effects of Platycodonis Radix-Curcumae Rhizoma on oral nanoparticle uptake and in vitro inhibition against breast cancer metastasis / 中国中药杂志

This study combined the herbal pair Platycodonis Radix-Curcumae Rhizoma(PR-CR) possessing an inhibitory effect on tumor cell proliferation and metastasis with the active component of traditional Chinese medicine(TCM) silibinin-loaded nanoparticles(NPs) with a regulatory effect on tumor microenvironment based on the joint effect on tumor cells and tumor microenvironment to inhi-bit cell metastasis. The effects of PR-CR on the cellular uptake of NPs and in vitro inhibition against breast cancer proliferation and metastasis were investigated to provide an experimental basis for improving nanoparticle absorption and enhancing therapeutic effects. Silibinin-loaded lipid-polymer nanoparticles(LPNs) were prepared by the nanoprecipitation method and characterized by transmission electron microscopy. The NPs were spherical or quasi-spherical in shape with obvious core-shell structure. The mean particle size was 107.4 nm, Zeta potential was-27.53 mV. The cellular uptake assay was performed by in vitro Caco-2/E12 coculture cell model and confocal laser scanning microscopy(CLSM), and the results indicated that PR-CR could promote the uptake of NPs. Further, in situ intestinal absorption assay by the CLSM vertical scanning approach showed that PR-CR could promote the absorption of NPs in the enterocytes of mice. The inhibitory effect of NPs on the proliferation and migration of 4T1 cells was analyzed using 4T1 breast cancer cells and co-cultured 4T1/WML2 cells, respectively. The results of the CCK8 assay showed that PR-CR-containing NPs could enhance the inhibition against the proliferation of 4T1 breast cancer cells. The wound healing assay indicated that PR-CR-containing NPs enhanced the inhibition against the migration of 4T1 breast cancer cells. This study enriches the research on oral absorption of TCM NPs and also provides a new idea for utilizing the advantages of TCM to inhibit breast cancer metastasis.

[Epidemiological characteristics of malaria in Liaoning Province from 1951 to 2014].

OBJECTIVE: To explore the epidemiological characteristics of malaria in Liaoning Province from 1951 to 2014, so as to provide the evidence for further control of the disease. METHODS: The data of registered malaria cases in Liaoning Province from 1951 to 2014 were collected and analyzed with epidemiological methods. RESULTS: From 1951 to 2014, there were reported malaria cases each year. The peaks of incidence were in 1953, 1962 and 1973, the incidence rates were 136.67/100 000, 256.81/100 000 and 35.89/100 000 respectively and the numbers of patients were 27 862, 65 460 and 11 523, respectively. From 1977 to 2014, the incidence rates were all less than 1/100 000. From 1951 to 1980, the occupations of patients were mainly farmers, and from 1981 to 2014 were mainly workers, farmers and exported laborers. CONCLUSIONS: The malaria endemic situation in Liaoning Province experienced the periods from outbreaks to basically elimination, and the long-term surveillance will be the further working emphasis.