RESUMO
Objective: To construct tissue microarrys in rats with ischemic brain injury and to provide an experimental means for the study of ischemic brain injury. Methods: Fifty male Sprague-Dawley rats were randomly assigned to cerebral ischemia 1-, 4-, 7-, 14- and 28-day groups, and each group had 8 rats. Sham-operation group (2 rats) was established at each time points. The model of global four-vessel occlusion was used. Cortex of temporal lobe, hippocampus and corpus callosum were selected as observing points. The tissue microarrys in ischemic brain injury rats were constructed in the order of designing microarrys, prefabricating recipient paraffin blocks, localizing donator paraffin blocks, sampling, slicing continuously and baking slice, etc. Results: One hundred and fifty lattice arrays with 15 × 10 were constructed on 36 min × 26 mm × 15 mm recipient paraffin blocks, and 150 tissue specimens were distributed, each lattice was 1.0 mm in diameter, and the microarrys were cut into 4 μm per layer. The lattice lattices distributed evenly under macroscopic observation, there were no fissuarings on paraffin blocks, and the point lattices did not fall off on microscope slide. Six point lattices displaced, and the displacement rate was 4%. HE stain showed sharp margin surrounding sampling tissues without obvious tissue crushing and deformation. Conclusion: The successful construction of tissue microarry may provide a convenient and efficient experimental means for the study of ischemic brain injury.
RESUMO
Objective To study the mRNA expression of myelin basic protein (MBP) and myelin oligodendregha glyeoprotein (MOG) in hippocampus of rats following global brain ischemia.Method The four- vessel occlusion animal model in the Sprague-Dawley rats was used in this study.The mRNA expression levels of MBP and MOG in the hippocampus of rats were analyzed by reverse transcription polymerase chain reaction (RT- PCR) at day 2,4,7,14 and 28 days after global brain ischemia.There were eight rats at each time-point and sham operated group.Results The mRNA expression of both MBP and MOG in hippocampus of rats decreased at 2 days after global brain ischemia.The gene expression of myelin gene decreased significantly at 7 days and it reached to the lowest level at 28 days.Compared with sham operated group,the gene expression of MBP and MOG in hippocampus of rats decreased significantly at 7,14 and 28 days after global brain ischemia (P
