Experience of thin layer chromatography separation and bioassay of platelet activating factor / 医学研究生学报

Objectives：To introduce the author's experiences in bioassay of platelet activating factor with thin layer chromatographic separation and platelet aggregation method,and to discuss probable problems in the process.Methods and Results：The method was used in determining portal venous blood samples from swine models of acute severe pancreatitis,control value were 0.79～1.65 pmol/L,in acute severe pancreatitis,mean blood level of platelet activating factor may be as high as 14.75 pmol/L.Conclusions:Thin layer chromatography separation and platelet aggregation assay were suitable for the determination of platelet activating factor,the results were satisfactory in case of standardized operation.