Hepatic transcriptome delineates the therapeutic effects of Sanren Tang on high-fat diet-induced non-alcoholic fatty liver disease.

OBJECTIVE: To evaluate the effects of Sanren Tang (SRT, ) on a high-fat diet (HFD)-induced non-alcoholic fatty liver disease (NAFLD) in mice and to investigate the hepatic transcriptome regulated by SRT. METHODS: The primary SRT components were identified using ultra-high-performance liquid chromatography-high-resolution accurate mass spectrometry. The SRT-induced pharmacological effects on HFD-induced NAFLD were evaluated in mice for 16 weeks. Obeticholic acid was used as a control drug. Body weight, food intake, and homeostatic model assessment for insulin resistance (HOMA-IR) index were analysed. Hepatic histological changes were observed in haematoxylin and eosin-stained sections and quantified using the NAFLD activity score (NAS). Serum alanine aminotransferase (ALT) and hepatic triglyceride (TG) levels were measured. Lipids in hepatocytes were visualised by Oil red staining. RNA-sequencing was performed to determine the transcriptome profile of the liver tissue. The differentially expressed genes were validated using real-time polymerase chain reaction and Western blotting. RESULTS: Four principal compounds were identified in the SRT: adenosine, amygdalin, luteoloside, and magnolol. SRT ameliorated hepatic histology and lipid deposition in the NAFLD mice, and decreased HOMA-IR, NAS and ALT, and hepatic TG levels. Hepatic transcriptome analysis revealed 232 HFD-regulated genes that were reversed by SRT simultaneously. Retinol metabolism, cytokine-cytokine receptor interaction, and peroxisome proliferator-activated receptor (PPAR) γ signalling were the top three SRT-regulated pathways in NAFLD. CONCLUSIONS: SRT significantly ameliorated HFD-induced NAFLD, which was correlated with the regulation of genes enriched in the retinol metabolism, cytokine-cytokine receptor interaction, and PPARγ signalling pathways.

Children pertussis IgG antibody levels analysis / 国际检验医学杂志

Objective In order to know the changes of serum pertussis IgG antibody level and the percent-age of white blood cells and lymphocytes in blood routine of children with IgG antibody positive,it is easy to diagnose and treat according to the clinical symptoms and examination results of children.Methods 485 cases of children in Shangdong Provincial Hospital were randomly enrolled as the test subjects,and pertussis anti-body IgG(PT -IgG)detection and blood cell detection were carried out.Results The levels of Pertussis IgG antibody were positive in 90 cases,the positive rate was 18.6%,pertussis IgG antibody level of children aged 8- <10 years old was the highest,2- <4 years old took the second place,10-14 years old were with the low-est levels.Among the patients with positive pertussis antibody levels,white blood cells in total were more than 20×109/L and lymphocyte percentage reached more than 60% in 19 cases,the positive rate was 21.1%,of which 6- <8 years old the highest positive rate was 10-14,the lowest.Conclusion IgG antibody of pertussis incidence is higher in children under 10 years old,the changes of white blood cells and lymphocytes mainly in children under the age of 8,the general course of the disease can be up to 2-3 months,so the need for early diagnosis and treatment,strengthen the immunity of pertussis vaccine is very necessary for children.