RESUMO
UNLABELLED: The effects of heat shock pretreatment on translocation and expression of alpha B-crystallin were evaluated in neonatal rat cardiomyocytes. RESULTS: 1. Western blot analysis demonstrated that cytosolic soluble alpha B-crystallin rapidly translocated to insoluble intracellular structure at 10 or 30 minutes and returned to cytosolic soluble compartment at about 1-2 h after heat shock. 2. Double staining of immunofluorescence showed that alpha B-crystallin redistributed in cytosol after heat shock, while HSC70 translocated from cytosol to nucleus. 3. Quantitative analysis with immunoelectronic microscopy demonstrated alpha B-crystallin translocated to cytoskeletal structure such as Z-line and nuclear envelope after heat shock. 4. Northern-blot showed that heat shock induced alpha B-crystallin mRNA expression. The rapid translocation and increasing expression of alpha B-crystallin after heat shock suggests that this small heat shock protein may play an important protective roles during the early and late phases of myocardial ischemic preconditioning.
