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1.
Plant Biotechnol J ; 4(3): 281-8, 2006 May.
Artigo em Inglês | MEDLINE | ID: mdl-17147634

RESUMO

Tomato mosaic virus vectors were designed that produced, by a translational readthrough, a fusion protein consisting of coat protein and metal-binding peptide, as a result of which particles were expected to present the metal-binding peptides on their surface. When inoculated in plants, they were expected to replicate and form a metal-adsorbing artificial sink in the cytoplasm, so as to reduce metal toxicity. Vectors were constructed harbouring sequences encoding various lengths of polyhistidine as a metal-binding peptide. One of the vectors, TLRT6His, which contains a 6 x histidine sequence, moved systemically in tobacco plants, and its particles were shown to retain cadmium ions by an in vitro assay. When a toxic amount of cadmium was applied, the toxic effect was much reduced in TLRT6His-inoculated tobacco plants, probably as a result of cadmium adsorption by TLRT6His particles in the cytosol. This shows the possible use of an artificial sink for metal tolerance and the advantage of employing a plant viral vector for phytoremediation.


Assuntos
Cádmio/metabolismo , Vetores Genéticos/metabolismo , Nicotiana/metabolismo , Tobamovirus/genética , Células Cultivadas , Plantas Geneticamente Modificadas/metabolismo , Protoplastos/metabolismo , Proteínas Recombinantes de Fusão/metabolismo , Proteínas Recombinantes de Fusão/fisiologia , Nicotiana/genética , Nicotiana/virologia , Tobamovirus/metabolismo
2.
Science ; 301(5631): 376-9, 2003 Jul 18.
Artigo em Inglês | MEDLINE | ID: mdl-12869764

RESUMO

We collected and completely sequenced 28,469 full-length complementary DNA clones from Oryza sativa L. ssp. japonica cv. Nipponbare. Through homology searches of publicly available sequence data, we assigned tentative protein functions to 21,596 clones (75.86%). Mapping of the cDNA clones to genomic DNA revealed that there are 19,000 to 20,500 transcription units in the rice genome. Protein informatics analysis against the InterPro database revealed the existence of proteins presented in rice but not in Arabidopsis. Sixty-four percent of our cDNAs are homologous to Arabidopsis proteins.


Assuntos
Genoma de Planta , Oryza/genética , Análise de Sequência de DNA , Processamento Alternativo , Sequência de Aminoácidos , Clonagem Molecular , Biologia Computacional , DNA Complementar , Bases de Dados de Ácidos Nucleicos , Bases de Dados de Proteínas , Genes de Plantas , Dados de Sequência Molecular , Fases de Leitura Aberta , Proteínas de Plantas/química , Proteínas de Plantas/genética , Proteínas de Plantas/fisiologia , Estrutura Terciária de Proteína , RNA Antissenso/genética , Homologia de Sequência de Aminoácidos , Homologia de Sequência do Ácido Nucleico , Fatores de Transcrição/química , Fatores de Transcrição/genética , Transcrição Gênica
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