RESUMO
The lipid composition of the adipose tissue from the bone marrow and that of the subcutaneous depot fat of four patients of membranous lipodystrophy were investigated. It was found that the lipid composition of the samples from the cases of membranous lipodystrophy was almost similar to that in controls. Also discussions were made on mechanism related to the accumulation of abnormal depot fat.
Assuntos
Tecido Adiposo/análise , Lipídeos/análise , Lipodistrofia/metabolismo , Adulto , Medula Óssea/análise , Feminino , Humanos , MasculinoRESUMO
The biological activity of a hot-water extract from delipidated BCG, designated as HSA (Hot-water Soluble Adjuvant), was investigated. The HSA did not induce hyperreactivity to bacterial endotoxin. The hot-water extract from which nucleic acids had been removed by streptomycin (SM-HSA) was found to enhance the delayed-type hypersensitivity as evidenced by the results of footpad reaction of mice. The HSA and SM-HSA could be injected to mice by an intraperitoneal route for 20 consecutive days without undesirable side effects. A comparative study was made on the effects of HSA in relation to the duration and doses of treatment with HSA using the ddI mice inoculated with Sarcoma-180. The most remarkable effect was observed when 0.25 mg of HSA had been injected for 20 consecutive days. Also SM-HSA was found to exert antitumor activity when applied in the same manner as above. These results suggest that the presence of nucleic acids is not related to the biological and antitumor activities of the hot-water extract.
Assuntos
Adjuvantes Imunológicos/isolamento & purificação , Mycobacterium bovis/imunologia , Adjuvantes Imunológicos/farmacologia , Adjuvantes Imunológicos/uso terapêutico , Animais , Vacina BCG/uso terapêutico , Endotoxinas/imunologia , Temperatura Alta , Masculino , Camundongos , Neoplasias Experimentais , Tamanho do Órgão , Salmonella enteritidis , Sarcoma 180 , Estreptomicina/farmacologia , Fatores de Tempo , ÁguaRESUMO
The antitumor activity of hot-water extract of delipidated BCG was investigated in mice inoculated with Sarcoma-180 cells and Ehrlich carcinoma cells, respectively. The hot-water extract was found to be effective when administered after and ineffective when administered before the inoculation of tumor cells. When this extract was given with anticancer drugs, such as Mitomycin C and cyclophosphamide, a combined effect was obtained in the treatment of Sarcoma-180 and of Ehrlich carcinoma.
Assuntos
Adjuvantes Imunológicos/uso terapêutico , Vacina BCG , Carcinoma de Ehrlich/terapia , Mycobacterium bovis/imunologia , Sarcoma 180/terapia , Animais , Ciclofosfamida/uso terapêutico , Quimioterapia Combinada , Feminino , Temperatura Alta , Imunoterapia , Injeções Intraperitoneais , Masculino , Camundongos , Mitomicinas/uso terapêutico , Mycobacterium bovis/análiseRESUMO
Syrian hamsters were exposed to the aerosol of a 5% solution of papain in the presence of cysteine and ethylenediamine-tetraacetic acid. Pulmonary emphysema in hamsters was observed after exposure to papain for two to three hours. There was no change with time in the severity of emphysema from the second through the fourth weeks after exposure to papain. Pathogenesis of pulmonary emphysema was discussed in relation to proteolysis of glycosaminoglycan-protein complex.