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1.
Curr Microbiol ; 81(7): 178, 2024 May 17.
Artigo em Inglês | MEDLINE | ID: mdl-38758299

RESUMO

A novel Gram-stain-negative, strictly aerobic, short-rod-shaped, and chemo-organoheterotrophic bacterium, designated KMU-50T, was isolated from seawater gathered from Dadaepo Harbor in South Korea. The microorganism grew at 0-4.0% NaCl concentrations (w/v), pH 6.0-8.0, and 4-37 °C. The 16S rRNA gene sequence-based phylogenetic tree demonstrated that the strain KMU-50T is a novel member of the family Roseobacteraceae and were greatly related to Aliiroseovarius crassostreae CV919-312T with sequence similarity of 98.3%. C18:1 ω7c was the main fatty acid and ubiquinone-10 was the only isoprenoid quinone. The dominant polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, two unidentified phospholipids, an unidentified aminolipid, and an unidentified lipid. The genome size of strain KMU-50T was 3.60 Mbp with a DNA G+C content of 56.0%. The average nucleotide identity (ANI) and average amino acid identity (AAI) values between the genomes of strain KMU-50T and its closely related species were 76.0-81.2% and 62.2-81.5%, respectively. The digital DNA-DNA hybridization (dDDH) value of strain KMU-50T with the strain of A. crassostreae CV919-312T was 25.1%. The genome of the strain KMU-50T showed that it encoded many genes involved in the breakdown of bio-macromolecules, thus showing a high potential as a producer of industrially useful enzymes. Consequently, the strain is described as a new species in the genus Aliiroseovarius, for which the name Aliiroseovarius salicola sp. nov., is proposed with the type strain KMU-50T (= KCCM 90480T = NBRC 115482T).


Assuntos
Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano , Ácidos Graxos , Fosfolipídeos , Filogenia , RNA Ribossômico 16S , Rhodobacteraceae , Água do Mar , Água do Mar/microbiologia , RNA Ribossômico 16S/genética , Rhodobacteraceae/genética , Rhodobacteraceae/classificação , Rhodobacteraceae/isolamento & purificação , Rhodobacteraceae/fisiologia , Ácidos Graxos/química , DNA Bacteriano/genética , República da Coreia , Fosfolipídeos/análise , Ubiquinona/química , Análise de Sequência de DNA , Genoma Bacteriano , Hibridização de Ácido Nucleico
2.
Antonie Van Leeuwenhoek ; 116(7): 631-641, 2023 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-37133556

RESUMO

A Gram-staining-negative, strictly aerobic, dark beige-colored, rod-shaped, chemoorganoheterotrophic, and catalase- and oxidase-positive bacterium, designated as KMU-90T, was isolated from coastal seawater in the Republic of Korea, and subjected to a polyphasic study. The novel isolate was able to grow at 0-6.0% NaCl concentrations (w/v), pH 6.5-9.5, and 4-45 °C. The 16S rRNA gene sequences-based phylogeny revealed that the novel marine isolate belongs to the family Roseobacteraceae of class Alphaproteobacteria and that it shared the greatest sequence similarity (97.3%) with Aestuariicoccus marinus NAP41T. The novel strain could be distinguished phenotypically from related representatives of the family Roseobacteraceae. The major (> 10%) fatty acids of strain KMU-90T were C18:1 ω7c and C18:1 ω7c 11-methyl and the only respiratory quinone was ubiquinone-10 (Q-10). Strain KMU-90T contained phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol, two unidentified aminolipids, an unidentified phospholipid, and three unidentified glycolipids as polar lipids. The assembled draft genome size of strain KMU-90T was 4.84 Mbp with a DNA G + C content of 66.5%. The average nucleotide identity, digital DNA-DNA hybridization, and average amino acid identity values between the genomes of strain KMU-90T and its closely related representatives were 77.0-79.0%, 14.6-20.0%, and 60.0-69.9%, respectively. From the polyphasic taxonomic results obtained, the strain is considered to represent a novel genus and a new species of the family Roseobacteraceae, for which the name Thetidibacter halocola gen. nov., sp. nov. is proposed. The type species is T. halocola, with the type strain KMU-90T (= KCCM 90287T = NBRC 113375T).


Assuntos
Fosfolipídeos , Água do Mar , RNA Ribossômico 16S/genética , DNA Bacteriano/genética , DNA Bacteriano/química , Análise de Sequência de DNA , Água do Mar/microbiologia , Fosfolipídeos/análise , Ácidos Graxos/análise , Filogenia , Técnicas de Tipagem Bacteriana
3.
Biochimie ; 204: 169, 2023 01.
Artigo em Inglês | MEDLINE | ID: mdl-36623908

RESUMO

This article has been retracted: please see Elsevier Policy on Article Withdrawal (https://www.elsevier.com/about/our-business/policies/article-withdrawal). This article has been retracted at the request of the Editor-in-Chief. The corresponding author, Dr Byoungduck Park, requested publication of a corrigendum to correct Fig. 2B which reused control data from a different publication (doi: 10.1016/j.intimp.2015.02.014). Upon further inspection, the Biochimie editorial team noticed that: Comparison of Fig. 2B with Fig. 4C of a previous publication in International Immunopharmacology by two co-authors (doi: 10.1016/j.intimp.2015.02.014) reveals that western blot ß-actin control data from the earlier paper were re-used in a different experiment shown in Fig. 2B of the article in Biochimie, after adjustment of the brightness/contrast. Furthermore, the same bands, after more image manipulation were presented as Smad3 data in Fig. 4C of the Biochimie article. Here the image manipulation involved notably the rotation of the set of bands by 180° and some adjustment of the height/width ratio. The authors apologise for any confusion that may have arisen from their article.

4.
Artigo em Inglês | MEDLINE | ID: mdl-35805580

RESUMO

In this study, the effects of a 12-week exercise program combining aerobic and resistance training on high-fat diet-induced obese Sprague Dawley (SD) rats after the injection of beta-amyloid into the cerebral ventricle were investigated. Changes in physical fitness, cognitive function, blood levels of beta-amyloid and metabolic factors, and protein expressions of neurotrophic factors related to brain function such as BDNF (brain-derived neurotrophic factor) in the quadriceps femoris, hippocampus, and cerebral cortex were analyzed. The subjects were thirty-two 10-week-old SD rats (DBL Co., Ltd., Seoul, Korea). The rats were randomized into four groups: ß-Non-Ex group (n = 8) with induced obesity and ßA25-35 injection into the cerebral ventricle through stereotactic biopsy; ß-Ex group (n = 8) with induced obesity, ßA25-35 injection, and exercise; S-Non-Ex group (n = 8) with an injection of saline in lieu of ßA25-35 as the control; and S-Ex group (n = 8) with saline injection and exercise. The 12-week exercise program combined aerobic training and resistance training. As for protein expressions of the factors related to brain function, the combined exercise program was shown to have a clear effect on activating the following factors: PGC-1α (peroxisome proliferator-activated receptor gamma coactivator 1-alpha), FNDC5 (fibronectin type III domain-containing protein 5), and BDNF in the quadriceps femoris; TrkB (Tropomyosin receptor kinase B), FNDC5, and BDNF in the hippocampus; PGC-1α, FNDC5, and BDNF in the cerebral cortex. The protein expression of ß-amyloid in the cerebral cortex was significantly lower in the ß-Ex group than in the ß-Non-Ex group (p < 0.05). The 12-week intervention with the combined exercise program of aerobic and resistance training was shown to improve cardiopulmonary function, muscular endurance, and short-term memory. The results demonstrate a set of positive effects of the combined exercise program, which were presumed to have arisen mainly due to its alleviating effect on ß-amyloid plaques, the main cause of reduced brain function, as well as the promotion of protein expressions of PGC-1α, FNDC5, and BDNF in the quadriceps femoris, hippocampus, and cerebral cortex.


Assuntos
Condicionamento Físico Animal , Treinamento Resistido , Peptídeos beta-Amiloides , Animais , Fator Neurotrófico Derivado do Encéfalo/metabolismo , Fibronectinas/metabolismo , Humanos , Obesidade/metabolismo , Obesidade/terapia , Condicionamento Físico Animal/fisiologia , Ratos , Ratos Sprague-Dawley
5.
Arch Microbiol ; 204(5): 273, 2022 Apr 21.
Artigo em Inglês | MEDLINE | ID: mdl-35449375

RESUMO

A bacterium, designated as KMU-166T, belonging to the class Gammaproteobacteria, was isolated from seawater collected on the coastline of Dadaepo, Republic of Korea. Strain KMU-166T was Gram-staining-negative, ovoid-shaped, motile, strictly aerobic, beige-colored, catalase-positive, and oxidase-negative; and had a chemoorganoheterotrophic metabolism. The novel isolate was found to grow at 1-4% NaCl concentrations (w/v), pH 6.5-9.5, and 10-40 °C. The 16S rRNA gene sequence-based phylogeny showed that strain KMU-166T affiliates to the family Spongiibacteraceae and that it shared the greatest sequence similarity (96.4%) with Spongiibacter marinus HAL40bT. The main (> 10%) cellular fatty acids were summed feature 3 (C16:1ω7c and/or C16:1ω6c), C17:1ω8c, and C18:1ω7c. The predominant respiratory quinone was ubiquinone-8 (Q-8). Strain KMU-166T comprised phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, and two unidentified lipids. The assembled draft genome was 4.40 Mbp in size with a DNA G+C content of 55.7%. The average nucleotide identity, digital DNA-DNA hybridization, and average amino acid identity values of KMU-166T and Spongiibacter marinus HAL40bT, Spongiibacter tropicus CL-CB221T, and "Spongiibacter pelagi" KMU-158T were found to be 77.7-79.8%, 13.7-15.4%, and 66.1-70.9%, respectively. From the distinguishable polyphasic taxonomic results obtained, the strain is considered to represent a novel species of the genus Spongiibacter for which the name Spongiibacter thalassae sp. nov. is proposed. The type strain of S. thalassae sp. nov. is KMU-166T (= KCCM 90449T = NBRC 114308T).


Assuntos
Gammaproteobacteria , Técnicas de Tipagem Bacteriana , DNA Bacteriano/genética , Ácidos Graxos/análise , Filogenia , RNA Ribossômico 16S/genética , Água do Mar/microbiologia , Análise de Sequência de DNA , Ubiquinona/química
6.
Antonie Van Leeuwenhoek ; 115(4): 487-495, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-35129702

RESUMO

A novel gammaproteobacterium, designated as KMU-158T, was isolated from seawater collected on the coastline of Dadaepo in the Republic of Korea, and characterized using a polyphasic taxonomic approach. Strain KMU-158T was Gram-staining-negative, pale beige-colored, strictly aerobic, rod-shaped, motile, and chemoorganoheterotrophic. The novel isolate was able to grow at 0-3% NaCl concentrations (w/v), pH 6.5-9.5, and 15-40 °C. The analysis based on 16S rRNA gene sequences indicated that strain KMU-158T belongs to the family Spongiibacteraceae and shared the highest similarity with Spongiibacter tropicus CL-CB221T (96.1%). The major cellular fatty acids were summed feature 3 (C16:1 ω7c and/or C16:1 ω6c) and C17:1 ω8c. The only respiratory quinone was identified as ubiquinone-8. The polar lipids of strain KMU-158T contained phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, and two unidentified lipids. The assembled draft genome size of strain KMU-158T was 3.29 Mbp with a DNA G + C content of 51.3%. The average nucleotide identity, digital DNA-DNA hybridization, and average amino acid identity values of KMU-158T and the representatives of the genus Spongiibacter were found to be 78.5-79.1%, 13.8-14.1%, and 66.6-66.8%, respectively. From the distinct phenotypic, phylogenetic, genomic, and chemotaxonomic properties, the strain KMU-158T is considered to represent a novel species of the genus Spongiibacter, for which the name Spongiibacter pelagi sp. nov. is proposed. The type strain of the species S. pelagi sp. nov. is KMU-158T (= KCCM 90448T = NBRC 114307T).


Assuntos
Ácidos Graxos , Água do Mar , Técnicas de Tipagem Bacteriana , DNA Bacteriano/genética , Ácidos Graxos/análise , Fosfolipídeos/análise , Filogenia , RNA Ribossômico 16S/genética , Água do Mar/microbiologia , Análise de Sequência de DNA
7.
Arch Microbiol ; 204(2): 125, 2022 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-34997854

RESUMO

A study based on a polyphasic taxonomic approach was carried out to identify and classify a novel marine alphaproteobacterium, designated as KMU-140T, isolated from coastal seawater collected at Jeju Island, Republic of Korea. Cells of strain KMU-140T were spherical, Gram-stain-negative, reddish-orange colored, strictly aerobic, catalase- and oxidase-positive, non-motile, and chemoorganoheterotrophic. The novel isolate was able to grow at NaCl concentrations of 0-5%, pH 6.0-9.5, and 10-45 °C. A phylogenetic analysis based on the 16S rRNA gene sequence showed that strain KMU-140T belongs to the family Erythrobacteraceae and was most closely related to Erythrobacter longus OCh101T (98.7%). Strain KMU-140T contained ubiquinone-10 (Q-10) as the only respiratory quinone and C18:1 ω7c, iso-C18:0, and C16:0 as the main (> 10%) cellular fatty acids. Strain KMU-140T produced carotenoid compounds that rendered the cell biomass a reddish-orange color. The assembled draft genome size of strain KMU-140T was 3.04 Mbp with G + C content of 60.6 mol%. The average nucleotide identity (ANI), digital DNA-DNA hybridization (dDDH), and average amino acid identity (AAI) values of KMU-140T and the species of the genus Erythrobacter were found to be 76.6-78.4%, 14.0-18.7%, and 69.6-77.8%, respectively. Phosphatidylethanolamine, phosphatidylglycerol, an unidentified phospholipid, and two unidentified lipids were identified as major polar lipids. On the basis of the polyphasic taxonomic features presented, the strain is considered to represent a novel species of the genus Erythrobacter for which the name Erythrobacter rubeus sp. nov. is proposed. The type strain of E. rubeus sp. nov. is KMU-140T (= KCCM 90479T = NBRC 115159T).


Assuntos
Sphingomonadaceae , Técnicas de Tipagem Bacteriana , Carotenoides , DNA Bacteriano/genética , Ácidos Graxos/análise , Filogenia , RNA Ribossômico 16S/genética , Água do Mar , Análise de Sequência de DNA , Sphingomonadaceae/genética
8.
Antonie Van Leeuwenhoek ; 114(9): 1407-1416, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34251526

RESUMO

A taxonomic investigation using a polyphasic method was conducted to identify a novel marine flavobacterium, designated as DJ-13T, isolated from the hard coral Acropora sp. collected at Okinawa, Japan. Bacterial cells were Gram-stain-negative, yellow-colored, strictly aerobic, rod-shaped, catalase- and oxidase-positive, non-motile, and chemoorganoheterotrophic. The novel isolate grew at NaCl concentrations of 0.5-7%, pH 6.5-9.0, and 15-37 °C. A phylogenetic study on the basis of the 16S rRNA gene sequence revealed that strain DJ-13T belongs to the family Flavobacteriaceae and that it shared the greatest sequence similarity (95.9%) with Croceivirga lutea CSW06T. Strain DJ-13T comprised iso-C17:0 3-OH, iso-C15:0, and iso-C15:1 G as the main (> 10%) cellular fatty acids. Menaquinone-6 (MK-6) was the only respiratory quinone. The assembled draft genome size of strain DJ-13T was 3.71 Mbp with G + C content of 38.7 mol%. The average nucleotide identity (ANI), digital DNA-DNA hybridization (dDDH), and average amino acid identity (AAI) values of DJ-13T and the species of the genus Croceivirga were found to be 74.9-75.5%, 13.4-14.7%, and 68.2-72.4%, respectively. Strain DJ-13T contained phosphatidylethanolamine, two unidentified aminolipids, and five unidentified lipids as polar lipids. From the polyphasic taxonomic results presented, the strain is considered to represent a novel species of the genus Croceivirga for which the name Croceivirga thetidis sp. nov. is proposed. The type strain of C. thetidis sp. nov. is DJ-13T (= KCTC 72790T = NBRC 114252T).


Assuntos
Antozoários , Flavobacteriaceae , Animais , Técnicas de Tipagem Bacteriana , DNA Bacteriano/genética , Ácidos Graxos/análise , Flavobacteriaceae/genética , Filogenia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Vitamina K 2
12.
FEMS Microbiol Lett ; 367(13)2020 07 01.
Artigo em Inglês | MEDLINE | ID: mdl-32573683

RESUMO

A novel alphaproteobacterium, designated as KMU-115T, was isolated from seawater collected on the coast of Chuam Beach, Republic of Korea, and subjected to a polyphasic characterization. The cells were pale apricot-colored, strictly aerobic, Gram-staining-negative, rod-shaped, non-motile and chemoorganoheterotrophic. The novel isolate grew at NaCl concentrations of 0-7%, pH 6.0-9.5 and 10-45°C. A phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain KMU-115T belongs to the family 'Rhodobacteraceae', and that it had the greatest sequence similarity (96.8%) with 'Roseibacterium beibuensis' JLT1202rT. The major respiratory quinone system of the novel isolate was ubiquinone-10 (Q-10) and the main (>10%) cellular fatty acid was C18:1 ω7c. The assembled draft genome size of strain KMU-115T was 3.71 Mbp. The genomic DNA G + C content was 70.8 mol%. Strain KMU-115T had phosphatidylglycerol, four unidentified aminolipids, an unidentified aminophospholipid and seven unidentified lipids as polar lipids. From the distinct polyphasic taxonomic characteristics, the strain is considered to represent a novel species of the genus Roseibacterium for which the name Roseibacterium persicicum sp. nov. is proposed. The type strain of R. persicicum sp. nov. is KMU-115T (= KCCM 90339T = NBRC 114190T). An emended description of the genus Roseibacterium is also provided.


Assuntos
Filogenia , Rhodobacteraceae/classificação , RNA Ribossômico 16S/genética , República da Coreia , Rhodobacteraceae/genética , Água do Mar/microbiologia , Especificidade da Espécie
13.
Sensors (Basel) ; 20(9)2020 Apr 29.
Artigo em Inglês | MEDLINE | ID: mdl-32365721

RESUMO

Localization is one of the key components in the operation of self-driving cars. Owing to the noisy global positioning system (GPS) signal and multipath routing in urban environments, a novel, practical approach is needed. In this study, a sensor fusion approach for self-driving cars was developed. To localize the vehicle position, we propose a particle-aided unscented Kalman filter (PAUKF) algorithm. The unscented Kalman filter updates the vehicle state, which includes the vehicle motion model and non-Gaussian noise affection. The particle filter provides additional updated position measurement information based on an onboard sensor and a high definition (HD) map. The simulations showed that our method achieves better precision and comparable stability in localization performance compared to previous approaches.

14.
FEMS Microbiol Lett ; 366(15)2019 08 01.
Artigo em Inglês | MEDLINE | ID: mdl-31511875

RESUMO

Polyphasic taxonomic analysis was performed on a novel marine bacterium, designated as strain YJ057T, isolated from marine sediment collected in the Republic of Korea. The strain was Gram-negative, beige-colored, facultatively anaerobic, coccoid or ovoid-shaped and nonmotile. Preliminary 16S rRNA gene sequence-based phylogenetic analysis indicated that this novel marine isolate belongs to the family Rhodobacteraceae of the class Alphaproteobacteria, and has the greatest (96.2%) sequence similarity to Paracoccus aestuariivivens GHD-30T. Major (>10%) fatty acids of strain YJ057T were C16:0 and C18:1 ω7c, G+C content in the genomic DNA of the strain was 63.6 mol% and the sole respiratory quinone was ubiquinone Q-10. It had phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine and some unidentified components (three aminolipids, a glycolipid, a phospholipid and two lipids). As per the distinct phylogenetic position and combination of phenotypic and genotypic traits, the strain is considered a novel species of the genus Paracoccus, and the name Paracoccus ravus sp. nov. is proposed.


Assuntos
Sedimentos Geológicos/microbiologia , Paracoccus/classificação , Paracoccus/isolamento & purificação , Filogenia , Água do Mar/microbiologia , DNA Bacteriano/genética , Ácidos Graxos/análise , Glicolipídeos/análise , Fosfolipídeos/análise , RNA Ribossômico 16S/genética , República da Coreia , Análise de Sequência de DNA , Ubiquinona/análogos & derivados , Ubiquinona/análise
15.
FEMS Microbiol Lett ; 366(15)2019 08 01.
Artigo em Inglês | MEDLINE | ID: mdl-31437270

RESUMO

A novel alphaproteobacterium, designated KMU-156T, was isolated from seawater collected on the coast of Jeju Island, Republic of Korea, and its phylogenetic position was determined using a polyphasic taxonomic approach. Strain KMU-156T was Gram-stain-negative, strictly aerobic, apricot-colored, rod-shaped, non-motile and chemoorganoheterotrophic. Phylogenetic study based on the 16S rRNA gene sequence revealed that the novel bacterium belongs to the family 'Rhodobacteraceae', of the class Alphaproteobacteria, and that it possessed the greatest sequence similarity (98.2%) with Tateyamaria omphalii MKT107T. DNA-DNA hybridization values between strains KMU-156T, T. omphalii KCTC 12333T and Tateyamaria pelophila DSM 17270T were less than 70%. The major isoprenoid quinone of the novel isolate was ubiquinone-10 (Q-10) and the major (> 10%) cellular fatty acids were C16:0 and C18:1 ω7c. The genomic DNA G + C content of strain KMU-156T was 59.3 mol%. The polar lipid profile of the strain KMU-156T had phosphatidylglycerol, phosphatidylcholine, an unidentified aminolipid, an unidentified phospholipid and two unidentified lipids. From the discriminative taxonomic features, the strain is considered to represent a novel species of the genus Tateyamaria for which the name Tateyamaria armeniaca sp. nov. is proposed. The type strain of T. armeniaca sp. nov. is KMU-156T (= KCCM 90321T = NBRC 113460T).


Assuntos
Filogenia , Rhodobacteraceae/classificação , Rhodobacteraceae/isolamento & purificação , Água do Mar/microbiologia , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/análise , Hibridização de Ácido Nucleico , Fosfolipídeos/análise , RNA Ribossômico 16S/genética , República da Coreia , Análise de Sequência de DNA , Ubiquinona/análogos & derivados , Ubiquinona/análise
16.
FEMS Microbiol Lett ; 366(11)2019 06 01.
Artigo em Inglês | MEDLINE | ID: mdl-31210259

RESUMO

The phylogenetic and taxonomic status of an alphaproteobacterium isolated from seawater, collected in the Republic of Korea, was elucidated based on a polyphasic method. Strain KMU-45T was Gram-stain-negative, strictly aerobic, rod-shaped, non-motile and chemoheterotrophic. Phylogenetic investigation based on the 16S rRNA gene sequence demonstrated that the novel marine isolate belongs to the family Erythrobacteraceae, of the class Alphaproteobacteria, and that it possessed the highest (98.7%) sequence similarity with Altererythrobacter ishigakiensis ATCC BAA-2084T. DNA-DNA hybridization values between strains KMU-45T and A. ishigakiensis KCTC 42446T were 31.4% ± 8.4%. The major isoprenoid quinone of the novel isolate was ubiquinone-10 (Q-10) and the major (>10%) cellular fatty acids were C17:1 ω8c, C17:1 ω6c and C18:1 ω7c. The genomic DNA G + C content of strain KMU-45T was 57.7 mol%. The polar lipid profile of the strain KMU-45T comprised phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, an unidentified phospholipid and an unidentified lipid. From the polyphasic taxonomic results in this study, the bacterium can be considered to represent a new species of the genus Altererythrobacter, for which the name Altererythrobacter rubellus sp. nov. is proposed. The type species is A. rubellus sp. nov., with the type strain KMU-45T (= KCCM 90270T = NBRC 112769T).


Assuntos
Alphaproteobacteria/genética , Alphaproteobacteria/classificação , Filogenia , RNA Ribossômico 16S/genética , Água do Mar/microbiologia
17.
Curr Microbiol ; 76(8): 963, 2019 08.
Artigo em Inglês | MEDLINE | ID: mdl-29736817

RESUMO

In the original version of this paper, the Chemotaxonomic Characteristics in the Results and Discussion section and legend of Table 2 given in the above paper are incorrect. These errors are corrected with this erratum.

18.
Arch Microbiol ; 201(4): 499-504, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30386885

RESUMO

A novel marine flavobacterial species, designated 3KA7-17T, was isolated from the hard coral Acropora formosa D. collected in Japan. The strain was pale-orange pigmented, Gram-stain negative, strictly aerobic, coccus shaped, and non-motile. Preliminary analysis based on the 16S rRNA gene sequence revealed an affiliation with the family Flavobacteriaceae of the phylum Bacteroidetes, and it had the greatest sequence similarity (96.0%) to Seonamhaeicola algicola Gy8T. The DNA G + C content was 34.3 mol%. MK-6 was the major menaquinone, with iso-C15:1 H and/or C13:0 3-OH (24.3%), iso-C15:0 (19.5%), iso-C15:0 3-OH (14.2%), and iso-C17:0 3-OH (15.9%) as the main (> 10%) cellular fatty acids. The major polar lipid profile consisted of phosphatidylethanolamine, two unidentified aminolipids, and two unidentified lipids. Based on distinct phylogenetic and phenotypic evidence, the strain represents a novel species of the genus Seonamhaeicola, for which the name Seonamhaeicola acroporae sp. nov. is proposed and the type strain is 3KA7-17T (= KCTC 62713T = NBRC 113410T).


Assuntos
Antozoários/microbiologia , Flavobacteriaceae/classificação , Animais , Composição de Bases , DNA Bacteriano/química , Ácidos Graxos/análise , Flavobacteriaceae/química , Flavobacteriaceae/isolamento & purificação , Lipídeos/análise , Filogenia , RNA Ribossômico 16S/genética , Vitamina K 2/análise
19.
Arch Microbiol ; 200(9): 1317-1322, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-29955925

RESUMO

A novel marine bacterium, designated 04OKA-3-218T, was isolated from the hard coral Galaxea fascicularis L. collected in Japan. The strain was dark-brown-pigmented, Gram-negative, strictly aerobic, curved-rod-shaped and non-motile. Phylogenetic analysis based on the 16S rRNA gene sequence showed the affiliation of the isolate with members of the family Flavobacteriaceae of the phylum Bacteroidetes, with the highest sequence similarity (95.2%) to Spongiibacterium pacificum SW169T. The DNA G+C content was 42.9 mol%; MK-6 was the major menaquinone; with iso-C17:0 3-OH (28.8%), iso-C15:0 (26.8%) and iso-C15:1 H and/or C13:0 3-OH (21.2%) as the main (>  10%) cellular fatty acids. The major polar lipid profile consisted of phosphatidylethanolamine, two unidentified aminolipids, two unidentified phosphoaminolipids and three unidentified lipids. On the basis of distinct phylogenetic and phenotypic evidences, the strain represents a novel species of the genus Spongiibacterium, for which the name Spongiibacterium fuscum sp. nov. is proposed. The type strain of S. fuscum sp. nov. is 04OKA-3-218T (= KCTC 62504T = NBRC 113248T).


Assuntos
Antozoários/microbiologia , Flavobacteriaceae/classificação , Flavobacteriaceae/isolamento & purificação , Animais , Técnicas de Tipagem Bacteriana , Composição de Bases/genética , Sequência de Bases , DNA Bacteriano/genética , Ácidos Graxos/análise , Flavobacteriaceae/genética , Japão , Fosfatidiletanolaminas/análise , Filogenia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Vitamina K 2/análise
20.
Arch Microbiol ; 200(8): 1159-1165, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-29872888

RESUMO

A novel marine bacterium, designated KMU-57T, was isolated from seawater collected from the Republic of Korea, and it was characterized using polyphasic taxonomic methods. Strain KMU-57T was Gram-stain-negative, strictly aerobic, rod-shaped, motile and dark-yellow-pigmented. Comparative analysis based on the 16S rRNA gene sequence showed the affiliation of the isolate with members of the family Flavobacteriaceae of the phylum Bacteroidetes, and it had the greatest sequence similarity (97.6%) to Lacinutrix jangbogonensis PAMC 27137T. The DNA-DNA relatedness value between strain KMU-57T and L. jangbogonensis PAMC 27137T was 37.8 ± 2.2%. The DNA G + C content of strain KMU-57T was 29.9 mol%; MK-6 was the major menaquinone with; iso-C15:1 G (18.6%) and C16:1 ω7c and/or C16:1 ω6c (15.8%) as the major (> 10%) cellular fatty acids. The polar lipid profile consisted of phosphatidylethanolamine, three unidentified aminolipids, and five unidentified lipids. The strain represents a novel species of the genus Lacinutrix for which the name Lacinutrix salivirga sp. nov. is proposed. The type strain of L. salivirga sp. nov. is KMU-57T (= KCTC 52878T = NBRC 112845T).


Assuntos
Flavobacteriaceae/classificação , Água do Mar/microbiologia , Composição de Bases , DNA Bacteriano/química , Ácidos Graxos/química , Flavobacteriaceae/química , Flavobacteriaceae/genética , Flavobacteriaceae/isolamento & purificação , Fosfatidiletanolaminas/análise , RNA Ribossômico 16S/genética , Vitamina K 2/análise
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