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1.
Chem Sci ; 11(33): 8989-8998, 2020 Aug 17.
Artigo em Inglês | MEDLINE | ID: mdl-34123153

RESUMO

Atomic vibrations due to stretching or bending modes cause optical phonon modes in the solid phase. These optical phonon modes typically lie in the frequency range of 102 to 104 cm-1. How much can the frequency of optical phonon modes be lowered? Herein we show an extremely low-frequency optical phonon mode of 19 cm-1 (0.58 THz) in a Rb-intercalated two-dimensional cyanide-bridged Co-W bimetal assembly. This ultralow frequency is attributed to a millefeuille-like structure where Rb ions are very softly sandwiched between the two-dimensional metal-organic framework, and the Rb ions slowly vibrate between the layers. Furthermore, we demonstrate temperature-induced and photo-induced switching of this low-frequency phonon mode. Such an external-stimulation-controllable sub-terahertz (sub-THz) phonon crystal, which has not been reported before, should be useful in devices and absorbers for high-speed wireless communications such as beyond 5G or THz communication systems.

2.
J Biochem ; 158(3): 189-95, 2015 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-25796064

RESUMO

A 40-kDa lectin with N-acetyl-d-glucosamine-binding ability was purified from the sera of fugu (Takifugu rubripes) by affinity chromatography and subsequent gel filtration. N-terminal amino acid sequencing, in silico cloning using the fugu genome database and cDNA cloning demonstrated that this lectin is a homologue of kalliklectin, a novel lectin that was previously found in the flathead teleost Platycephalus indicus and has structural similarity to mammalian plasma kallikreins and coagulation factor XI. This is the second report of a kalliklectin, but the fugu kalliklectin differs in its sugar-binding spectra, intersubunit association and tissue distribution from the previously identified flathead kalliklectin. These findings indicate that kalliklectins vary in properties among fish species.


Assuntos
Acetilglucosamina/química , Calicreínas/química , Lectinas/química , Filogenia , Acetilglucosamina/metabolismo , Sequência de Aminoácidos , Animais , DNA Complementar/genética , Fator XI/química , Fator XI/metabolismo , Calicreínas/genética , Lectinas/sangue , Lectinas/genética , Dados de Sequência Molecular , Homologia de Sequência de Aminoácidos , Takifugu/sangue , Takifugu/genética
3.
Dev Comp Immunol ; 41(1): 20-6, 2013 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-23562572

RESUMO

N-acetyl-d-glucosamine (GlcNAc) is one of the components of peptidoglycan, a biopolymer in the bacterial cell wall. We purified a novel GlcNAc-binding protein, designated as fGBP-78, from sera of fugu (Takifugu rubripes). The fGBP-78 is a heteromer of 78- and 25-kDa subunits. Moreover, fGBP-78 exerted remarkable inhibitory effects on the growth of both Gram-positive and Gram-negative bacteria, including ones virulent for marine fish species as well as non-pathogenic Escherichia coli. These results suggest that fGBP-78 contributes to bacterial clearance in fugu. Furthermore, the nanoLC-MS/MS and Western blotting analyses reveal that the 78-kDa subunit is the fugu IgM heavy chain. In addition, the molecular mass of the other subunit (25 kDa) was equal to that of the Ig light chain. Overall, results indicate that fGBP-78 is an IgM molecule presumably acts as a natural antibody. This paper reports a novel function of teleost IgM as a significant suppresser against bacterial growth.


Assuntos
Anticorpos Antibacterianos/imunologia , Proteínas de Transporte/imunologia , Proteínas de Peixes/imunologia , Imunoglobulina M/imunologia , Subunidades Proteicas/imunologia , Takifugu/imunologia , Acetilglucosamina/química , Acetilglucosamina/imunologia , Sequência de Aminoácidos , Animais , Anticorpos Antibacterianos/sangue , Anticorpos Antibacterianos/farmacologia , Proteínas de Transporte/sangue , Proteínas de Transporte/farmacologia , Proteínas de Peixes/sangue , Proteínas de Peixes/farmacologia , Bactérias Gram-Negativas/efeitos dos fármacos , Bactérias Gram-Negativas/crescimento & desenvolvimento , Bactérias Gram-Positivas/efeitos dos fármacos , Bactérias Gram-Positivas/crescimento & desenvolvimento , Imunidade Inata , Imunoglobulina M/sangue , Imunoglobulina M/farmacologia , Dados de Sequência Molecular , Ligação Proteica , Multimerização Proteica , Subunidades Proteicas/sangue , Subunidades Proteicas/farmacologia , Takifugu/sangue , Takifugu/genética , Takifugu/microbiologia
4.
Dev Biol ; 377(1): 275-83, 2013 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-23357540

RESUMO

Nanos is a translational regulator required for the survival and maintenance of primordial germ cells during embryogenesis. Three nanos homologs are present in the genome of the sea urchin Strongylocentrotus purpuratus (Sp), and each nanos mRNA accumulates specifically in the small micromere (sMic) lineage. We found that a highly conserved element in the 3' UTR of nanos2 is sufficient for reporter expression selectively in the sMic lineage: microinjection into a Sp fertilized egg of an RNA that contains the GFP open reading frame followed by Sp nanos2 3'UTR leads to selective reporter enrichment in the small micromeres in blastulae. The same result was seen with nanos2 from the sea urchin Hemicentrotus pulcherrimus (Hp). In both species, the 5'UTR alone is not sufficient for the sMic localization but it always increased the sMic reporter enrichment when present with the 3'UTR. We defined an element conserved between Hp and Sp in the nanos2 3'UTR which is necessary and sufficient for protein enrichment in the sMic, and refer to it as GNARLE (Global Nanos Associated RNA Lability Element). We also found that the nanos2 3'UTR is essential for the selective RNA retention in the small micromeres; GNARLE is required but not sufficient for this process. These results show that a combination of selective RNA retention and translational control mechanisms instills nanos accumulation uniquely in the sMic lineage.


Assuntos
Regiões 3' não Traduzidas/genética , Proteínas de Transporte/genética , Linhagem da Célula/genética , Células Germinativas/citologia , Hemicentrotus/citologia , Hemicentrotus/genética , Strongylocentrotus purpuratus/citologia , Strongylocentrotus purpuratus/genética , Animais , Pareamento de Bases/genética , Sequência de Bases , Proteínas de Transporte/metabolismo , Sequência Conservada/genética , Genes Reporter , Dados de Sequência Molecular , Nucleotídeos/genética , Biossíntese de Proteínas/genética , Sequências Reguladoras de Ácido Ribonucleico/genética , Deleção de Sequência
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