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1.
J Intellect Disabil Res ; 66(4): 323-331, 2022 04.
Artigo em Inglês | MEDLINE | ID: mdl-35040230

RESUMO

BACKGROUND: Some patients with intellectual disabilities (ID) are prescribed antipsychotic drugs for symptomatic treatment of behavioural disorders. Nevertheless, it can still prove difficult to perform dental treatments safely for some patients with ID. In such cases, treatment under intravenous sedation (IVS) is one option. Sedative, hypnotic and α-blocking effects of antipsychotic drugs may cause adverse events, such as severe hypotension, among patients who take antipsychotic drugs regularly. This study aimed to investigate the effects of oral antipsychotic medication on cardiovascular function during IVS. Accordingly, we compared mean blood pressure (MBP) and heart rate (HR) between patients who regularly take antipsychotic drugs and patients who do not. METHODS: Thirty-seven patients with ID were enrolled in this study. All participants were outpatients of Special Care Dentistry of general hospital and received dental treatment under IVS performed with a combination of midazolam and propofol. Eighteen patients regularly took antipsychotics (medication group), and 19 patients were not currently taking antipsychotics (non-medication group). MBP, HR, dose, and effect-site concentration of intravenous sedative medications were measured at three points: 'before IVS', 'at optimal sedation', and 'during dental treatment'. RESULTS: The magnitude of reduction of MBP was significantly smaller in the medication group than in the non-medication group (P < 0.023). However, there were no differences in MBP, HR, dose, and effect-site concentration of midazolam and propofol between groups at any point. CONCLUSION: These results suggest that antipsychotic medication may not have clinically significant adverse effects on cardiovascular fluctuations during dental treatment under IVS for persons with ID.


Assuntos
Antipsicóticos , Deficiência Intelectual , Propofol , Antipsicóticos/farmacologia , Antipsicóticos/uso terapêutico , Sedação Consciente/efeitos adversos , Sedação Consciente/métodos , Assistência Odontológica , Humanos , Hipnóticos e Sedativos/efeitos adversos , Deficiência Intelectual/tratamento farmacológico , Deficiência Intelectual/etiologia , Midazolam/efeitos adversos , Midazolam/farmacologia , Midazolam/uso terapêutico , Propofol/efeitos adversos
2.
Toxicol Lett ; 293: 9-15, 2018 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-29702200

RESUMO

In August 2003, 44 victims were poisoned by chemical warfare agents (CWAs) leaked from five drums that were excavated at a construction site in Qiqihar, Northeast China. The drums were abandoned by the former Japanese imperial army during World War II and contained a mixture of Sulfur mustard (SM) and Lewisite. We carried out a total of six regular check-ups between 2006 and 2014, and from 2008 we added neurological evaluations including neuropsychological test and autonomic nervous function test in parallel with medical follow-up as much as was possible. Severe autonomic failure, such as hyperhidrosis, pollakiuria, diarrhoea, diminished libido, and asthenia appeared in almost all victims. Polyneuropathy occurred in 35% of the victims and constricted vision occurred in 20% of them. The rates of abnormal response on cold pressor test (CPT), active standing test (AST), Heart rate variability (CVR-R), performed in 2014, were 63.1%, 31.6%, and 15.9%, respectively. On neuropsychological testing evaluated in 2010, a generalized cognitive decline was observed in 42% of the victims. Memories and visuospatial abilities were affected in the remaining victims. Finally, a 17-item PTSD questionnaire and the Beck Depression Inventory evaluated in 2014 revealed long-lasting severe PTSD symptoms and depression of the victims. Our findings suggest that an SM/Lewisite compound have significant adverse consequences directly in cognitive and emotional network and autonomic nervous systems in the brain.


Assuntos
Intoxicação por Arsênico/história , Arsenicais , Substâncias para a Guerra Química/intoxicação , Guerra Química/psicologia , Transtornos Mentais/induzido quimicamente , Transtornos Mentais/psicologia , Gás de Mostarda/intoxicação , Doenças do Sistema Nervoso/induzido quimicamente , Doenças do Sistema Nervoso/psicologia , II Guerra Mundial , Adulto , Arsenicais/história , Povo Asiático , Doenças do Sistema Nervoso Autônomo/induzido quimicamente , Guerra Química/história , Substâncias para a Guerra Química/história , China , Feminino , História do Século XX , Humanos , Masculino , Pessoa de Meia-Idade , Força Muscular/efeitos dos fármacos , Gás de Mostarda/história , Testes Neuropsicológicos , Escalas de Graduação Psiquiátrica , Transtornos de Estresse Pós-Traumáticos/psicologia , Adulto Jovem
3.
Biochemistry ; 40(50): 15184-93, 2001 Dec 18.
Artigo em Inglês | MEDLINE | ID: mdl-11735401

RESUMO

Stimulation of death receptors (Fas on human T-cell leukemia Jurkat cells and tumor necrosis factor receptor-1 on human monoblastic leukemia U937 cells) triggers the specific degradation of 28S ribosomal RNA, and this process may contribute to cell death through the inhibition of protein synthesis. We have developed an analytical method using a polyacrylamide-agarose composite gel to evaluate ribosomal subunits in apoptotic cells (human breast carcinoma MCF-7 cells treated with staurosporine and human 293T cells irradiated with ultraviolet light were used in addition to the two apoptosis systems described above). No alterations were detected by this method, suggesting that apoptosis, including the process of ribosomal RNA degradation, does not cause fragmentation or extensive conformational changes in the ribosome. We also examined the status of 21 different ribosomal proteins in apoptotic cells by immunoblotting with polyclonal antibodies. S11 was specifically downregulated in apoptotic MCF-7 cells and in other apoptotic breast carcinoma cells. Previous studies have shown that S11 is heterogeneously expressed in cancer cells. Taken together, it appears that particular intracellular environments regulate the expression of S11 protein. However, the mechanism by which this process is modulated is as yet unknown. Furthermore, we have demonstrated that our composite gel electrophoresis system can efficiently detect ubiquitination of ribosomal subunits.


Assuntos
Neoplasias da Mama/tratamento farmacológico , Neoplasias da Mama/metabolismo , Proteínas Ribossômicas/metabolismo , Estaurosporina/farmacologia , Sequência de Aminoácidos , Animais , Apoptose , Neoplasias da Mama/genética , Neoplasias da Mama/patologia , Regulação para Baixo/efeitos dos fármacos , Eletroforese em Gel de Ágar , Eletroforese em Gel de Poliacrilamida , Feminino , Humanos , Células Jurkat , Masculino , Dados de Sequência Molecular , Ratos , Ratos Sprague-Dawley , Proteínas Ribossômicas/genética , Ribossomos/efeitos dos fármacos , Ribossomos/metabolismo , Células Tumorais Cultivadas , Células U937 , Ubiquitina/metabolismo
4.
Br J Pharmacol ; 134(7): 1498-504, 2001 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-11724756

RESUMO

1. We investigated the ability of a newly synthesized sugar derivative, OJ-R9188, [N-(2-tetradecylhexadecanoyl)-O-(L-alpha-fucofuranosyl)-D-seryl]-L-glutamic acid 1-methylamide 5-L-arginine salt, to block binding of selectins to their ligands in vitro and inhibit the infiltration of leukocytes in vivo. 2. OJ-R9188 prevented the binding of human E-, P- and L-selectin-IgG fusion proteins to immobilized sialyl Lewis(x) (sLe(x))-pentasaccharide glycolipid, with IC(50) values of 4.3, 1.3, and 1.2 microM, respectively. 3. In a mouse model of thioglycollate-induced peritonitis, OJ-R9188 at 10 mg kg(-1), i.v. inhibited neutrophil accumulation in the peritoneal cavity. In the IgE-mediated skin reaction, OJ-R9188 at 3 and 10 mg kg(-1), i.v. significantly inhibited extravasation of neutrophils and eosinophils into the inflammatory sites and at 10 mg kg(-1), i.v. also inhibited infiltration caused by picryl chloride-induced delayed-type hypersensitivity in mice. These results suggest that OJ-R9188 may be a useful selectin blocker, with activity against human and mouse E-, P- and L-selectins in vitro and in vivo, and that blocking selectin-sLe(x) binding is a promising strategy for the treatment of allergic skin diseases.


Assuntos
Desoxiguanosina/farmacologia , Dermatite/prevenção & controle , Sequestradores de Radicais Livres/farmacologia , Hipersensibilidade Tardia/prevenção & controle , Selectinas/metabolismo , Animais , Anti-Inflamatórios/farmacologia , Ligação Competitiva/efeitos dos fármacos , Desoxiguanosina/análogos & derivados , Desoxiguanosina/uso terapêutico , Dermatite/imunologia , Dermatite/metabolismo , Dexametasona/farmacologia , Relação Dose-Resposta a Droga , Selectina E/metabolismo , Sequestradores de Radicais Livres/uso terapêutico , Humanos , Hipersensibilidade Tardia/induzido quimicamente , Hipersensibilidade Tardia/metabolismo , Imunoglobulina G/metabolismo , Selectina L/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Oligossacarídeos/metabolismo , Selectina-P/metabolismo , Peritonite/induzido quimicamente , Peritonite/metabolismo , Peritonite/prevenção & controle , Cloreto de Picrila/administração & dosagem , Polissacarídeos/farmacologia , Proteínas Recombinantes de Fusão/metabolismo , Antígeno Sialil Lewis X , Tioglicolatos/administração & dosagem
5.
Jpn J Cancer Res ; 91(8): 802-10, 2000 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-10965021

RESUMO

Mutants of model eukaryotic organisms have revealed that most ribosomal proteins are essential for cell viability. However, the precise functional role of each ribosomal protein is largely unknown. Recent reports on the involvement of ribosomal proteins in various genetic diseases and studies on the extraribosomal functions of these proteins have cast some light on their localization and functions. Here we prepared rabbit polyclonal antibodies against 26 human ribosomal proteins; each of these reagents recognized a single band in immunoblots of the purified ribosome. We used these antibodies to evaluate a panel of human cancer cell lines. Although no deficiency of ribosomal proteins was observed, the abundance of S11 and S30 varied substantially among the cell lines, but the difference did not affect the biogenesis or composition of the ribosome. Therefore, the heterogeneity may be related to extraribosomal functions of S11 and S30. The antibodies described here are powerful tools for research into the molecular mechanisms of protein translation, cell-biological and medical studies on the ribosomal proteins, and ultimately a comprehensive understanding of all ribosomal proteins (rising dbl quote, left (low)ribosomics").


Assuntos
Anticorpos/imunologia , Proteínas Ribossômicas/imunologia , Humanos , Immunoblotting , Peptídeos/imunologia , Proteínas Ribossômicas/análise , Células Tumorais Cultivadas
6.
Br J Pharmacol ; 131(8): 1531-6, 2000 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-11139428

RESUMO

Selectins play an important role on leukocytes infiltration into inflammatory tissues. To understand the role of selectins, we investigated the effects of selectin-IgG chimeras and anti selectin antibodies on the murine IgE-mediated skin inflammation model. Biphasic skin reactions were induced by intradermal challenge with ovalbumin (OA) to ears of actively sensitized mice. This reaction was characterized by immediate and late phase responses observed as which were induced via a rapid increase in capillary permeability and leukocyte infiltration, respectively. The expression of E-selectin mRNA was significantly increased to reach its highest level at 2 h after OA challenge. E-, P-, and L-selectin-IgG chimeras inhibited the late phase responses, i.e. ear swelling, neutrophil infiltration and eosinophil infiltration at 24 h after OA challenge in a dose-dependent manner at dose range of 0.1 - 10 mg kg(-1), i.v. Antiselectin antibodies did not inhibit the increase of ear swelling. But anti E- and P-selectin antibodies significantly inhibited neutrophil infiltration and eosinophil infiltration. These results indicate that selectins play an important role on the late phase response of the murine IgE-mediated skin inflammation model by mediating inflammatory cell adhesion to endothelium.


Assuntos
Imunoglobulina E/imunologia , Selectinas/fisiologia , Pele/imunologia , Animais , Anticorpos Monoclonais/farmacologia , Relação Dose-Resposta a Droga , Selectina E/genética , Selectina E/imunologia , Selectina E/farmacologia , Orelha , Edema/enzimologia , Edema/genética , Edema/prevenção & controle , Peroxidase de Eosinófilo , Feminino , Regulação da Expressão Gênica/efeitos dos fármacos , Hipersensibilidade Tardia/imunologia , Imunoglobulina G/genética , Imunoglobulina G/farmacologia , Inflamação/patologia , Inflamação/prevenção & controle , Injeções Subcutâneas , Selectina L/genética , Selectina L/imunologia , Selectina L/farmacologia , Camundongos , Camundongos Endogâmicos BALB C , Ovalbumina/administração & dosagem , Ovalbumina/imunologia , Selectina-P/genética , Selectina-P/imunologia , Selectina-P/farmacologia , Peroxidase/efeitos dos fármacos , Peroxidase/metabolismo , Peroxidases/efeitos dos fármacos , Peroxidases/metabolismo , RNA Mensageiro/efeitos dos fármacos , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Proteínas Recombinantes de Fusão/farmacologia , Selectinas/genética , Selectinas/farmacologia , Pele/efeitos dos fármacos , Pele/patologia , Fatores de Tempo
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