RESUMO
To investigate the significance of oropharyngeal flora and gastric flora in elderly patients receiving nasogastric tube feeding, throat secretions and gastric aspirates were cultured and the pH of the latter was measured. Of 116 bacterial isolates from throat secretions of 27 elderly patients, 30 were beta-streptococci and 28 were Pseudomonas aeruginosa. Bacteria isolated from gastric aspirates numbered 86 and 24 (27.9%) of them were the same species as those found in the throat secretions. Patients with gastric pH were below 3.5 had significantly lower concentrations of gram-negative bacili in gastric aspirates. We also studied oropharyngeal flora in 33 elderly patients who were admitted to Nagoyashi Koseiin Geriatric Hospital. The major bacterial isolates from throat swabs of bedridden patients were gram-negative bacilli and beta-streptococci, especially group B streptococci (GBS). We measured the level of antibody to GBS in these patients. Those from whom GBS were isolated had high titers. These results suggest that in elderly patients receiving enteral nasogastric) tube feeding, large numbers of bacteria colonize the oral cavity and stomach. The measurement of type-specific antibody to GBS may be useful in managing such patients.
Assuntos
Nutrição Enteral/efeitos adversos , Boca/microbiologia , Faringe/microbiologia , Pseudomonas aeruginosa/isolamento & purificação , Estômago/microbiologia , Streptococcus agalactiae/isolamento & purificação , Idoso , Idoso de 80 Anos ou mais , Anticorpos Antibacterianos/análise , Enterococcus/isolamento & purificação , Feminino , Humanos , Masculino , Infecções Respiratórias/microbiologia , Streptococcus agalactiae/imunologiaRESUMO
The promoter of TGF-beta type II receptor lacks TATA box or CAAT box. In order to define the exact transcriptional start site(s), we used "oligo-capping" method developed by ourselves. The major transcripts were started from GAA located between -35 and -33 relative to 5' end of cDNA. Other minor transcripts started from -4, +11 and +17. Deletion analysis of this promoter region revealed that it contained two adjacently located promoters (P1 and P2) capable of acting by itself. The P1 is located between -22 and +55 which covers the minor transcriptional start sites described above. The P2 is located just upstream of the P1 between -137 and -22. The results showed all of the transcripts were started either from P1 or P2.
Assuntos
Regiões Promotoras Genéticas , Receptores de Fatores de Crescimento Transformadores beta/biossíntese , Receptores de Fatores de Crescimento Transformadores beta/genética , Transcrição Gênica , Animais , Sequência de Bases , Linhagem Celular , Clonagem Molecular , Humanos , Luciferases/biossíntese , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida , Oligodesoxirribonucleotídeos , Proteínas Serina-Treonina Quinases , RNA Mensageiro/análise , RNA Mensageiro/biossíntese , Receptor do Fator de Crescimento Transformador beta Tipo II , Proteínas Recombinantes/biossíntese , Deleção de Sequência , TATA Box , TransfecçãoRESUMO
We report the successful closure of a recurrent bronchial fistula using a combination gastric seromuscular patch and omental pedicle flap. This new method provided an immediate airtight closure of the bronchial fistula. This technique appears superior to closure by omentum alone.
Assuntos
Fístula Brônquica/cirurgia , Retalhos Cirúrgicos/métodos , Fístula Brônquica/diagnóstico por imagem , Humanos , Masculino , Pessoa de Meia-Idade , Radiografia , RecidivaRESUMO
We have combined epitope tagging with an expression cDNA library in order to isolate cDNAs encoding nuclear proteins. This system allows us to detect proteins expressed from the cDNA library by using antibodies against the epitope tag. As a tag, we used the 85-aa N-terminal peptide of the SV40 T antigen which lacks the nuclear localization signal (NLS). A strong expression vector, pEF204 [Kim et al., Gene 91 (1990) 217-223], was modified into an epitope-tagging vector, pTkim, by putting the tag-coding region and a cDNA cloning site immediately after its promoter. From cDNA libraries constructed using pTkim, we isolated eight cDNA clones whose tagged proteins were localized within the nuclei. From partial sequence analysis, two cDNAs were shown to code for the ribosomal (r-) proteins, simian L44 and human L21, and the others were shown to be new. Furthermore, six cDNAs including those encoding the r-proteins could direct a non-karyophilic T antigen [Fischer-Fantuzzi et al., Virology 153 (1986) 87-95] into nuclei, showing that they have NLSs. These results indicate that this system is useful for isolating new cDNAs which code for nuclear proteins.
Assuntos
DNA/genética , DNA/isolamento & purificação , Epitopos/análise , Biblioteca Gênica , Proteínas Nucleares/genética , Animais , Antígenos Transformantes de Poliomavirus/análise , Antígenos Transformantes de Poliomavirus/genética , Antígenos Transformantes de Poliomavirus/imunologia , Linhagem Celular , Clonagem Molecular , Cicloeximida/farmacologia , Epitopos/genética , Vetores Genéticos , Células HeLa , Humanos , Proteínas Nucleares/análise , Proteínas Nucleares/imunologia , Plasmídeos , Poli A/genética , Poli A/isolamento & purificação , RNA/genética , RNA/isolamento & purificação , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Proteínas Recombinantes de Fusão/análise , Proteínas Recombinantes de Fusão/imunologia , Mapeamento por Restrição , Deleção de Sequência , Vírus 40 dos Símios/genética , TransfecçãoRESUMO
Ofloxacin (OFLX) or Enoxacin (ENX) was intramuscularly administered at a dose of 100 mg/kg to rats (normal and pneumonia model). Bronchoalveolar lavage (BAL) was performed at 30 min after administration and concentrations of OFLX or ENX in the recovery fluid were determined. Total recovery amounts of OFLX from 10 BAL procedures were 64.7 +/- 18.7 micrograms in the normal group and 126.5 +/- 16.1 micrograms in the pneumonia group, and the amounts of ENX were 15.6 +/- 4.6 micrograms in the normal group and 32.8 +/- 6.1 micrograms in the pneumonia group. Regarding the ratio of total recovery amounts in BAL to serum concentrations of OFLX or ENX, the pneumonia group was higher than the normal group and the ratio of OFLX was higher than that of ENX. Regarding the ratio of total recovery amount in BAL to the amount in lung parenchyma, the ratio of OFLX was higher than that of ENX, but there was no significant difference between the normal group and the pneumonia group in the ratio of OFLX or ENX. With regard to the ratio of the amount of OFLX or ENX in lung parenchyma to their serum concentrations, the ratio was higher in the pneumonia group than in the normal group, but there was no significant difference between OFLX and ENX. Based on the above results, it was concluded that the permeability of OFLX into epithelium of blood capillary is the same as that of ENX, but the permeability of OFLX into alveolus epithelium is superior to that of ENX.(ABSTRACT TRUNCATED AT 250 WORDS)
Assuntos
Enoxacino/farmacocinética , Ofloxacino/farmacocinética , Pneumonia/metabolismo , Alvéolos Pulmonares/metabolismo , Animais , Líquido da Lavagem Broncoalveolar/metabolismo , Permeabilidade Capilar , Enoxacino/sangue , Ofloxacino/sangue , Ratos , Ratos EndogâmicosRESUMO
The case of a 58-year-old woman with a primary adenocarcinoma of the duodenojejunal junction and an extraluminal growth is reported. The patient's chief complaint had been lumbago and emaciation, and a tumor in her left upper abdomen was found. Hypotonic duodenography showed a filling defect with ulceration of the duodenojejunal junction. Angiography revealed irregular vessels and a slight tumor stain. A CT scan further revealed that this tumor had invaded into the abdominal aorta with paraaortic lymphadenopathies.
