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1.
Am J Chin Med ; 46(6): 1169-1185, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30286626

RESUMO

Chronic kidney disease (CKD) is an increasing major public health problem worldwide. The number of CKD patients on hemodialysis is growing rapidly as well. Acupuncture technique is one of the traditional Chinese medicine methods and has been used in a variety of diseases. Nowadays, the clinical application of acupuncture technique for CKD patients has become the focus for its effectiveness and security. In this paper, we will review the therapeutic effects and mechanisms of different acupuncture techniques for CKD patients. In patients with CKD, acupuncture improves renal function, reduces proteinuria, controls hypertension, corrects anemia, relieves pain, and controls many hemodialysis-related complications such as uremic pruritus, insomnia and fatigue. The mechanisms are related to the regulation of sympathetic nerve and the activation of bioactive chemicals. In conclusion, acupuncture is proved to be beneficial for CKD patients. More research, however, is needed to verify the potential mechanisms.


Assuntos
Terapia por Acupuntura , Insuficiência Renal Crônica/terapia , Terapia por Acupuntura/métodos , Anemia/etiologia , Anemia/terapia , Fadiga/etiologia , Fadiga/terapia , Humanos , Hipertensão/etiologia , Hipertensão/terapia , Manejo da Dor , Proteinúria/etiologia , Proteinúria/terapia , Prurido/etiologia , Prurido/terapia , Diálise Renal/efeitos adversos , Insuficiência Renal Crônica/complicações , Distúrbios do Início e da Manutenção do Sono/etiologia , Distúrbios do Início e da Manutenção do Sono/terapia , Sistema Nervoso Simpático , Uremia/etiologia , Uremia/terapia
2.
Cell Physiol Biochem ; 46(2): 451-460, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29614506

RESUMO

BACKGROUND/AIMS: Renal tubular epithelial cells and fibroblasts are the main sources of myofibroblasts, and these cells produce the extracellular matrix during tubulointerstitial fibrosis (TIF). Histone deacetylases (HDAC) inhibitors exert an antifibrogenic effect in the skin, liver and lung. Sirtuin 2 (SIRT2), which is a class III HDAC, is an important member of NAD+-dependent protein deacetylases. The current study evaluated the role of SIRT2 in renal TIF. METHODS: Immunohistochemical staining and Western blot were performed to evaluate SIRT2 expression in TIF patients and unilateral urethral obstruction (UUO) mice. Western blot was used to assess the protein levels of SIRT2, α-SMA, collagen III, fibronectin, and MDM2 in tubular epithelial cells and fibroblasts. The specific inhibitor AGK2 was used to inhibit SIRT2 activity, and targeted siRNA was used to suppress SIRT2 expression. RESULTS: SIRT2 expression increased in the tubulointerstitium of TIF patients and UUO mice. SIRT2 inhibition ameliorated TIF in UUO mice. SIRT2 expression in tubular cells was unchanged after exposure to TGF-ß1. The SIRT2-specifc inhibitor AGK2 did not attenuate TGF-ß1-induced tubular epithelial-mesenchymal transition. However, SIRT2 was upregulated in fibroblasts, and fibroblasts were activated after TGF-ß1 treatment. Genetic knockdown and chemical inhibition of SIRT2 attenuated TGF-ß1-induced fibroblast activation. We also explored the downstream signaling of SIRT2 during fibroblast activation. Genetic knockdown and chemical inhibition of SIRT2 suppressed TGF-ß1-induced increase in MDM2 expression, and inhibition of the MDM2-p53 interaction using Nutlin-3 did not suppress SIRT2 upregulation. CONCLUSION: Our results suggest that SIRT2 participates in the activation of fibroblasts and TIF, which is mediated via regulation of the MDM2 pathway, and the downregulation of SIRT2 may be a therapeutic strategy for renal fibrosis.


Assuntos
Proteínas Proto-Oncogênicas c-mdm2/metabolismo , Sirtuína 2/metabolismo , Animais , Linhagem Celular , Regulação para Baixo/efeitos dos fármacos , Células Epiteliais/citologia , Células Epiteliais/metabolismo , Transição Epitelial-Mesenquimal/efeitos dos fármacos , Fibroblastos/citologia , Fibroblastos/metabolismo , Fibrose , Furanos/farmacologia , Humanos , Imidazóis/metabolismo , Nefropatias/patologia , Túbulos Renais/citologia , Masculino , Camundongos , Piperazinas/metabolismo , Quinolinas/farmacologia , Interferência de RNA , Ratos , Sirtuína 2/antagonistas & inibidores , Sirtuína 2/genética , Fator de Crescimento Transformador beta1/farmacologia , Proteína Supressora de Tumor p53/metabolismo , Regulação para Cima/efeitos dos fármacos , Obstrução Ureteral/metabolismo , Obstrução Ureteral/patologia , Obstrução Ureteral/veterinária
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