[Effects of Aerobic Carbon Sources on Biofilm with Simultaneous Phosphate Removal and Enrichment].

In a cyclic alternating O/A operation mode, phosphorus accumulating organisms (PAOs) can undertake phosphate removal and enrichment as the main process in wastewater treatment plants. The effects of the concentration of carbon sources during the aerobic stage on phosphate removal and enrichment performance of PAO biofilms, and the microbial population structure in the biofilms, were investigated. The results showed that the aerobic COD concentration decreased from 200 mg·L-1 to 0 mg·L-1, the phosphorus uptake rate improved by 1.29 times, the phosphorus concentration in effluent stabilized below 0.5 mg·L-1, the phosphorus release rate increased by 3.56 times, and the phosphate concentration in the circulating solution increased from 27.125 mg·L-1 to 55.91 mg·L-1. With respect to the change in microbial communities, the identification showed that the abundance of Proteobacteria increased by approximately two times, and the enrichment effects of Rhodocyclaceae and Anaerolineaceae increased by 2.28 and 5 times, respectively. Reducing the concentration of the carbon source in the aerobic section was beneficial to the screening and enrichment of PAOs, strengthening the removal of phosphate in the aerobic section and the release of phosphate in the anaerobic section. This resulted in an enriched phosphate solution. These observations provide a theoretical basis for future urban sewage treatment plants seeking to reduce their carbon demand.

[Analysis of CANON Process Start-up with Fiber Carrier].

A CANON reactor with fiber carrier was started up by seeding nitrification sludge and ANAMMOX sludge to study the operating characteristics of a fiber carrier. The results showed that total nitrogen removal load rose from 0.09 kg·(m3·d)-1 to 0.9 kg·(m3·d)-1 and remained steady in the 85th day. This indicated that fiber carrier is beneficial to the accumulation of sludge, and the reactor can maintain a higher biomass. The DO in the reactor reached 5 mg·L-1 with the enrichment of microorganisms, biofilm thickening, and the improvement of the reactor's ability. The DO gradient of the biofilm from the outside to the inside was 0.32-0 mg·L-1, which could be obtained by a microelectrode. It was shown that the permeability of oxygen to the biofilm decreased, and the amount of nitrifying microorganisms decreased with biofilm thickening. The quantitative PCR results showed that the abundance of ANAMMOX was an order of magnitude more than before. The abundance of AOB increased slightly, while the abundance of NOB stayed at a relatively low level.