The Mycological and Molecular Biological Studies on Arthroderma benhamiae Isolated for the First Time in Korea / 대한의진균학회지

BACKGROUND: Arthroderma (A.) benhamiae, one of three telemorphs of Trichophyton (T.) mentagrophytes, has not been isolated until recently in Korea, but in Japan the first report on it was made in 1998. OBJECTIVE: To identify A. benhamiae for the first time in Korea. METHODS: Twelve strains suggestive of A. benhamiae grossly and microscopically were detected among 1, 059 of T. mentagrophytes isolated at the Catholic Skin Clinic, Daegu, from 1998 to 2000. They were examined by mating tests and molecular methods. In mating tests, they were respectively crossed with "+" and "-" tester strains of A. vanbreuseghemii, A. benhamiae African race, A. benhamiae Americano-European race. Molecular methods included the sequence analysis of internal transcribed spacer 2 (ITS2) region of ribosomal DNA and chitin synthase 1 (CHS1) gene, and random amplified DNA polymorphism (RAPD) with random primer OPAO-15 (5'-GAA GGC TCC C-3'). RESULTS: Mating tests revealed that 12 strains of T. mentagrophytes consisted of 6 of A. benhamiae, 4 of A. vanbreuseghemii and 2 of indeterminate. Six strains of A. benhamiae, all isolated from the patients with a history of contact with rabbits, included 1 of Americano-European race and 5 of African race of Americano-European race and 5 of African race. One clinical isolate, a strain of A. benhamiae Americano-European race "-" in mating tests, was almost identical with the standard strains of A. benhamiae Americano-European race with molecular methods as 99.2% (351/354) similarity of ITS2 sequence, 99.1% (313/ 316) similarity of CHS1 sequence, and similar RAPD pattern. Five clinical strains of A. benhamiae African race were identical with standard strains in ITS2 and CHS1 gene and RAPD pattern. CONCLUSION: The first isolation of 6 strains of A. benhamiae in Korea was reported. Five were identified as A. benhamiae African race "+", and 1 as A. benhamiae Americano-European race "-".

The Mycological and Molecular Biological Studies on Arthroderma benhamiae Isolated for the First Time in Korea / 대한의진균학회지

BACKGROUND: Arthroderma (A.) benhamiae, one of three telemorphs of Trichophyton (T.) mentagrophytes, has not been isolated until recently in Korea, but in Japan the first report on it was made in 1998. OBJECTIVE: To identify A. benhamiae for the first time in Korea. METHODS: Twelve strains suggestive of A. benhamiae grossly and microscopically were detected among 1, 059 of T. mentagrophytes isolated at the Catholic Skin Clinic, Daegu, from 1998 to 2000. They were examined by mating tests and molecular methods. In mating tests, they were respectively crossed with "+" and "-" tester strains of A. vanbreuseghemii, A. benhamiae African race, A. benhamiae Americano-European race. Molecular methods included the sequence analysis of internal transcribed spacer 2 (ITS2) region of ribosomal DNA and chitin synthase 1 (CHS1) gene, and random amplified DNA polymorphism (RAPD) with random primer OPAO-15 (5'-GAA GGC TCC C-3'). RESULTS: Mating tests revealed that 12 strains of T. mentagrophytes consisted of 6 of A. benhamiae, 4 of A. vanbreuseghemii and 2 of indeterminate. Six strains of A. benhamiae, all isolated from the patients with a history of contact with rabbits, included 1 of Americano-European race and 5 of African race of Americano-European race and 5 of African race. One clinical isolate, a strain of A. benhamiae Americano-European race "-" in mating tests, was almost identical with the standard strains of A. benhamiae Americano-European race with molecular methods as 99.2% (351/354) similarity of ITS2 sequence, 99.1% (313/ 316) similarity of CHS1 sequence, and similar RAPD pattern. Five clinical strains of A. benhamiae African race were identical with standard strains in ITS2 and CHS1 gene and RAPD pattern. CONCLUSION: The first isolation of 6 strains of A. benhamiae in Korea was reported. Five were identified as A. benhamiae African race "+", and 1 as A. benhamiae Americano-European race "-".

A case of neurofibromatosis with unusual clinical manifestations / 대한피부과학회지

No abstract available.

A Case of Bullous Pemphigid in a Patient with Chronic Renal Failure / 대한피부과학회지

No abstract available.

Histiocytosis-X with Chronic Weeping Ulcers in the Anogenital Areas

No abstract available.

Distribution of Fibronectin in the Skin of Basal cell Carcinoma , Squamous cell Carcinoma , and Psoriasis / 대한피부과학회지

Fibronectin(FN) is a major component of the extracellular matrix and is able to bind to cells and other components of the matrix. Although the cell producing the largest amounts of fibronectin is fibroblast, the pro duct,ion on FN also has been described in cultured keratinocyt,e and epithelial tumor cells of basal cell carcinoma(BCC). Recently, functional role nf FN in relation to biologic behavior of BCC and squamous cell carcinoma(SCC) is open to speculation. The authors investigated the localization of FN in the lesional skin of 5 cases of BCC, 4 of SCC, 5 of psoriasis and 5 normal skin using direct immunofluorescence technique with antifibronectin antibody to find out the production of FN in keratinocytes and tumor cells. 1. In the skin of BCC, FN was presented in a thick, linear depositions along the margin of tumor lobules in all cases, and as fillamentous deposits or scattered points in the nest of tumor cells in 3 cases. 2. In the skin of SCC, FN was presented in a thin, coarse depositions around the margin of tumor but not presented within the tumor nest in all cases. R. In psoriasis, FN was observed in horney layer and upper part of epidermis. It was presented more abundantly in dermo-epidermal junction, papillary and reticular dermis than in normal skin. 4. In normal skin, FN was absent in the epidermis but presented in dermo-epidermal junction and blood vessel wall of upper dermis, the amount of FN was decreased from papillary to lower dermis.