A strategy to maximize astaxanthin production from Haematococcus pluvialis in a cost-effective process by utilizing a PBR-LGP-PBR array (PLPA) hybrid system using light guide panel (LGP) and solar cells.

This study evaluated economic feasibility through production efficiency, return on investment (ROI) and payout time of a hybrid system using a photobioreactor (PBR)-light guide panel (LGP)-PBR array (PLPA) and solar cells developed for astaxanthin and ω-3 FA simultaneous production of Haematococcus pluvialis. The economic feasibility of the PLPA hybrid system (8 PBRs) and the PBR-PBR-PBR array (PPPA) system (8 PBRs) was evaluated for producing high-value products while effectively reducing CO2. Introducing a PLPA hybrid system has increased the amount of culture per area by 1.6 times. Also, the shading effect was effectively suppressed with an LGP placed between each PBR, increasing biomass and astaxanthin productivity by 3.39-fold and 4.79-fold, respectively compared to the untreated H. pluvialis cultures. In addition, ROI increased by 6.55 and 4.71 times, and the payout time was reduced by 1.34 and 1.37 times, respectively in 10 and 100-ton scale processes.

Enhanced astaxanthin production of Haematococcus pluvialis strains induced salt and high light resistance with gamma irradiation.

This study was conducted to increase the productivity of biomass that contains high astaxanthin content by developing a mutant Haematococcus pluvialis strain with strong environmental tolerance. H. pluvialis has a low cell-growth rate and is vulnerable to stressors such as salinity or light intensity, which may hinder large-scale commercial cultivation. A mutant M5 strain selected through 5000-Gy gamma irradiation showed improved biomass and astaxanthin production under high-salinity and high-light intensity conditions. With enhanced SOD activity and overexpressed astaxanthin biosynthesis genes (lyc, crtR-b, bkt2), M5 demonstrated an increase in biomass and astaxanthin productivity by 86.70 % and 66.15 %, respectively compared to those of untreated cells. Also, the omega-3 content of M5 increased by 149.44 % under 40 mM CaCl2 compared to the untreated cells. Finally, even when subjected to high-intensity light irradiation for the whole life cycle, the biomass and astaxanthin concentration increased by 84.99 % and 241 %, respectively, compared to the wild-type cells.

Microalgae-derived hydrogen production towards low carbon emissions via large-scale outdoor systems.

Hydrogen as a clean fuel is receiving attention because it generates only water and a small amount of nitrogen oxide upon combustion. Biohydrogen production using microalgae is considered to be a highly promising carbon-neutral technology because it can secure renewable energy while efficiently reducing CO2 emissions. However, previous studies have mainly focused on improving the biological performance of microalgae; these approaches have struggled to achieve breakthroughs in commercialization because they do not heavily consider the complexity of the entire production process with microalgae, including large-scale cultivation, biomass harvest, and biomass storage. This work presents an in-depth analysis of the state-of-the-art technologies focused on large-scale cultivation systems with efficient downstream processes. Considering the individual processes of biohydrogen production, strategies are discussed to minimize carbon emissions and improve productivity simultaneously. A comprehensive understanding of microalgae-derived biohydrogen production suggests future directions for realizing environmental and economic sustainability.

Novel effective bioprocess for optimal CO2 fixation via microalgae-based biomineralization under semi-continuous culture.

In this study, the effects of microalgae-based biomineralization in a semi-continuous process (M-BSP) on biomass productivity and CO2 fixation rate were investigated. M-BSP significantly improved biomass production and CO2 fixation rate at the second stage of induction by sustaining relatively high photosynthetic rate without exposure to toxic substances (e.g., chlorellin) from aging cells using the microalgae Chlorella HS2. In conventional systems, cells do not receive irradiated light evenly, and many cells age and burst because of the long culture period. In contrast, in the M-BSP, the photosynthesis efficiency increases and biomass production is not inhibited because most of the cells can be harvested during shorter culture period. The accumulated biomass production and CO2 fixation rate of the HS2 cells cultured under M-BSP increased by 4.67- (25 ± 1.09 g/L) and 10.9-fold (30.29 ± 1.79 g/L day-1), respectively, compared to those cultured without the CaCl2 treatment.

Waste mitigation and resource recovery from food industry wastewater employing microalgae-bacterial consortium.

Wastewater generated by the food industry is rich in nitrogen and phosphorus with possible presence of heavy metals. Physical and chemical methods of treatment, although effective, are expensive and may cause secondary environmental pollution damaging aquatic and human life. Traditional biological methods are eco-friendly and cost-effective but involve standalone microorganisms that pose risk of contamination and are not as effective. This review discusses the application of novel microalgal-bacterial consortium as a solution for the resource recovery and treatment of dairy, starch and aquaculture wastewater. Use of biofilm reactors containing anaerobic and aerobic sludge has shown 80-90% and > 90% COD and nutrient removal efficiency in treatment of dairy and starch processing wastewater, respectively. The treatment of aquaculture processing wastewater can be challenging due to high sality and requires salt-tolerant bacteria-microalgae consortium. In this regard, the identification of dominant microalgae and bacteria using 16S rRNA and 18S rRNA genes is recommended.

Effective contamination control strategies facilitating axenic cultivation of Haematococcus pluvialis: Risks and challenges.

With industrialization, anthropogenic mishandlings have resulted in the discharge of abundant amount of CO2 into the atmosphere. This has triggered an unnatural warming that has dramatically increased the Earth's temperature in a short duration. This problem can be addressed by the biological conversion of CO2; several studies have been conducted using H. pluvialis culture that produces high value-added materials, such as astaxanthin and omega-3 fatty acids. However, although H. pluvialis has a high market value, the market size is quite small. Because H. pluvialis cells are susceptible to contamination due to its slow growth rate, hence large-scale culture of H. pluvialis without reliable contamination control strategies poses significant risks. This review comprehensively discusses the contamination that occurs during the culturing of H. pluvialis in various culture systems under different culture conditions. The review also discusses the strategies in controlling the biotic contaminants, such as bacteria and fungi.

Sustainable production of polyhydroxybutyrate from autotrophs using CO2 as feedstock: Challenges and opportunities.

Due to industrialization and rapid increase in world population, the global energy consumption has increased dramatically. As a consequence, there is increased consumption of fossil fuels, leading to a rapid increase in CO2 concentration in the atmosphere. This accumulated CO2 can be efficiently used by autotrophs as a carbon source to produce chemicals and biopolymers. There has been increasing attention on the production of polyhydroxybutyrate (PHB), a biopolymer, with focus on reducing the production cost. For this, cheaper renewable feedstocks, molecular tools, including metabolic and genetic engineering have been explored to improve microbial strains along with process engineering aspects for scale-up of PHB production. This review discusses the recent advents on the utilization of CO2 as feedstock especially by engineered autotrophs, for sustainable production of PHB. The review also discusses the innovations in cultivation technology and process monitoring while understanding the underlying mechanisms for CO2 to biopolymer conversion.

Concurrent enhancement of CO2 fixation and productivities of omega-3 fatty acids and astaxanthin in Haematococcus pluvialis culture via calcium-mediated homeoviscous adaptation and biomineralization.

Haematococcus pluvialis has attracted interest as a bio-platform for producing omega-3 fatty acids (ω-3 FA) and astaxanthin that have a great potential as anti-inflammatory drugs. This study aimed to concurrently enhance the CO2 fixation and the productivities of ω-3 FA and astaxanthin, which have been difficult to achieve because of the dissimilar culture methods for each goal, via calcium-mediated homeoviscous adaptation and biomineralization. As a result of 3 mM of Ca2+ addition, ω-3 FA content was improved by 31% due to Ca2+-induced homeoviscous adaptation. Biomineralization was promoted by the extracellular carbonic anhydrase, which resulted in 46.3% improvement in CO2 fixation. CaCO3 from the biomineralization was beneficially re-used in the H. pluvialis culture and triggered 178- and 522-fold increased biomass productivity and astaxanthin content, respectively, thanks to its anisotropic nature. The Ca2+-based productivity enhancement strategy was applied to large-scale culture which resulted improvement in overall bioprocess performance.

Robust cyst germination induction in Haematococcus pluvialis to enhance astaxanthin productivity in a semi-continuous outdoor culture system using power plant flue gas.

The microalgae Haematococcus pluvialis biologically converts CO2 into natural astaxanthin that possesses a strong antioxidant activity; its low carbon footprint and economic viability have allowed it to garner great attention. However, low efficiency of the cultivation process is a major hurdle in its commercial production. Here, additional nitrogen was provided to fully grown cells to induce efficient cyst germination, thereby rapidly increasing the number of cells containing astaxanthin. After germination, these cells were rapidly converted from zooids to cysts by nitrogen depletion, while maintaining a steady astaxanthin content of 5.5%. Consequently, a 2.1-fold increase in astaxanthin productivity in comparison with that in a batch culture was achieved. Moreover, the germination-based semi-continuous process yielded 2.6 times higher astaxanthin productivity in a large-scale culture using power plant flue gas and a polymeric photobioreactor. This study provides a promising cultivation strategy for the commercial mass production of natural astaxanthin.

A green decontamination technology through selective biomineralization of algicidal microorganisms for enhanced astaxanthin production from Haematococcus pluvialis at commercial scale.

Currently, there is a lack of an efficient, environmentally-benign and sustainable industrial decontamination strategy to steadily achieve improved astaxanthin production from Haematococcus pluvialis under large-scale outdoor conditions. Here, this study demonstrates for the first time that a CaCO3 biomineralization-based decontamination strategy (CBDS) is highly efficient in selectively eliminating algicidal microorganisms, such as bacteria and fungi, during large-scale H. pluvialis cultivation under autotrophic and mixotrophic conditions, thereby augmenting the astaxanthin productivity. Under outdoor AT and MT conditions, the average astaxanthin productivity of H. pluvialis using CBDS in a closed photobioreactor system was substantially increased by 14.85- (1.19 mg L-1 d-1) and 13.65-fold (2.43 mg L-1 d-1), respectively, compared to the contaminated H. pluvialis cultures. Given the exponentially increasing demand of astaxanthin, a natural anti-viral, anti-inflammatory, and antioxidant drug, CBDS will be a technology of interest in H. pluvialis-based commercial astaxanthin production which has been hindered by the serious biological contaminations.

Sedimentation rate-based screening of oleaginous microalgae for utilization as a direct combustion fuel.

The co-combustion of microalgae biomass with coal has the potential to significantly reduce CO2 emissions by eliminating expensive and carbon-emitting downstream processes. In this study, the utilization of microalgal biomass as a direct combustion fuel in co-firing industries and the screening of potential oleaginous strains of high calorific value was investigated. High-lipid accumulating mutants were selected from mutant mixtures based on cell density using differential sedimentation rates. Of the mutant strains obtained in the top phase of the separation medium, 72% showed a higher lipid content than the wild-type strain. One mutant strain exhibited a 57.3% enhanced lipid content and a 9.3% lower heating value (LHV), both indicators of direct combustion fuel performance, compared to the wild-type strain. Our findings indicate that sedimentation rate-based strain selection allows for the easy and rapid screening of high-lipid content algal strains for the use of microalgae as direct combustion fuels.

Enhanced biomass and lipid production of Neochloris oleoabundans under high light conditions by anisotropic nature of light-splitting CaCO3 crystal.

The aim of this work was to study the anisotropic effect of crystalline CaCO3 nanoparticles (CN)-driven multiple refraction/scattering from the CN-coated agglomerated cells on the rate of photosynthesis and the product yield under high light conditions in the freshwater microalgae Neochloris oleoabundans. The CN-coating via biomineralization significantly improved the biomass and lipid production of N. oleoabundans during second stage of autotrophic induction by sustaining relatively high rate of photosynthesis at high irradiance using the multiple-splitting effect of the anisotropic polymorphism. The CN were successfully produced, adsorbed and grown on the external cells under conditions of mild alkalinity (pH 7.5-8.0), mild CaCl2 concentration (0.05 M) and under nitrogen starvation with strong light (400 µE m-2 s-1). Consequently, lipid content and productivity of N. oleoabundans cells cultured with 0.05 M CaCl2 increased by 18.4% and 31.5%, respectively, compared to the cells cultured with 0.05 M CaCl2 and acetazolamide to inhibit calcification.