Periodontitis Is Associated With Heart Failure: A Population-Based Study (NHANES III).

Objectives: The aim of this study was to investigate the relationship between periodontitis and heart failure using the Third National Health and Nutrition Examination Survey (NHANES III). Methods: Participants who had received a periodontal examination were included and investigated for the occurrence of heart failure. The included participants were divided into no/mild periodontitis and moderate/severe periodontitis groups according to their periodontal status. Weighted prevalence of heart failure was calculated, and weighted logistic regressions models were used to explore the association between periodontitis and heart failure. Possible influencing factors were then explored through subgroup analysis. Results: Compared with that of the no/mild periodontitis group, the incidence of heart failure in participants with moderate/severe periodontitis was 5.72 times higher (95% CI: 3.76-8.72, p < 0.001). After adjusting for gender, age, race, body mass index, poverty income ratio, education, marital status, smoking status, drinking status, hypertension, diabetes, stroke, and asthma, the results showed that the incidence of heart failure in the moderate/severe group was 3.03 times higher (95% CI: 1.29-7.13, p = 0.012). Subgroup analysis showed that criteria, namely, male, 40-60 years old, non-Hispanic white, body mass index >30, poverty income ratio ≥1, not more than 12 years of education, currently drinking, stroke but no diabetes, or asthma supported moderate/severe periodontitis as a risk factor for heart failure (p < 0.05). Conclusion: According to data from this nationally representative sample from the United States, periodontitis is associated with an increased risk of heart failure.

Comprehensive analysis of interleukin-8 gene polymorphisms and periodontitis susceptibility.

BACKGROUND: Associations between interleukin-8 (IL-8) gene polymorphisms and periodontitis susceptibility have been investigated in many published studies, but the conclusions are still inconsistent. Therefore, we performed this systematic review and meta-analysis to review which polymorphisms have been researched and to obtain a precise result of the same polymorphism from different studies. RESULTS: Finally 10 publications involving 1938 patients and 1569 controls were yielded, including 12 polymorphisms. Six studies investigated rs4073 polymorphism; two focused on rs2227306 and rs2227307; two referred to rs2227532 and T-738A; one detected rs2230054, rs1126579 and rs1126580; one inspected A2767T, T11722T2 and C1633T, and one for rs2234671 polymorphism. Of them, IL-8 C1633T and rs1126580 polymorphisms showed positive association while the other ten polymorphisms revealed negative results. MATERIALS AND METHODS: A comprehensive literature search from PubMed, Web of Science, and Chinese National Knowledge Infrastructure was conducted for all potentially relevant studies published before January 2, 2017. Two authors selected the studies and extracted data. The pooled analysis was conducted using the RevMan 5.1 software if a polymorphism was reported by two or more studies. CONCLUSIONS: Based on current evidence, the IL-8 rs4073, A2767T, T11722T2, rs2234671, rs2230054, rs1126579, rs2227306, rs2227307, rs2227532, and T-738A polymorphisms were not associated with periodontitis susceptibility; the IL-8 C1633T and rs1126580 polymorphisms were associated with increased risk of periodontitis.

[The influence of Co-Cr-Mo alloys on biological behaviour of L929 mouse fibroblasts].

PURPOSE: To investigate the influence of dental Co-Cr-Mo alloys on biological behaviour of L929 mouse fibroblasts. METHODS: Leaching liquor of medical pure titanium, Co-Cr-Mo alloys, Co-Cr alloys and Ni-Cr alloys was prepared and used to culture the L929 cells for 24 h, 48 h and 72 h, respectively; then the growth of L929 cells was observed under inverted phase contrast microscope, and the cytotoxicity grades of 4 kinds of materials were evaluated by using CCK-8 test. The apoptosis of L929 cells was measured by flow cytometry(FCM) and acridine orange staining was used to observe the L929 cells adhered on the surface of samples under fluorescent microscope. The data was statistically analyzed with SPSS17.0 software package. RESULTS: Under inverted phase contrast microscope, at each time point, L929 cells grew in a good condition except in the Ni-Cr alloys group that minor karyopyknosis was indicating slight celluar cytotoxicity. Optical density (OD), apoptosis rate and cell adhesion number in Co-Cr-Mo alloys group at each time point were significantly less than in medical pure titanium group (P<0.05), and higher than in Co-Cr alloys group (P<0.05) and Ni-Cr alloys group (P<0.05). During observation period, the cytotoxicity grades of medical pure titanium group, Co-Cr-Mo alloys group and Co-Cr alloys group was grade 1, while Ni-Cr alloy group was grade 2, i.e. mild cytotoxicity. CONCLUSIONS: Co-Cr-Mo alloys have no adverse effect on the biological behavior of L929 cells, which may be suitable for dental clinical application with good biocompatibility.