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1.
BMC Microbiol ; 21(1): 76, 2021 03 08.
Artigo em Inglês | MEDLINE | ID: mdl-33685392

RESUMO

BACKGROUND: Anthrax and smallpox are high-risk infectious diseases, and considered as potential agents for bioterrorism. To develop an effective countermeasure for these diseases, we constructed a bivalent vaccine against both anthrax and smallpox by integrating a gene encoding protective antigen (PA) of Bacillus anthracis to the genome of the attenuated vaccinia virus strain, KVAC103. RESULTS: Immunization with this bivalent vaccine induced antibodies against both PA and vaccinia virus in a mouse model. We also observed that the efficacy of this vaccine can be enhanced by combined immunization with immunoadjuvant-expressing KVAC103. Mouse groups co-immunized with PA-expressing KVAC103 and either interleukin-15 (IL-15) or cholera toxin subunit A (CTA1)-expressing KVAC103 showed increased anti-PA IgG titer and survival rate against B. anthracis spore challenge compared to the group immunized with PA-expressing KVAC103 alone. CONCLUSIONS: We demonstrated that the attenuated smallpox vaccine KVAC103 is an available platform for a multivalent vaccine and co-immunization of immunoadjuvants can improve vaccine performance.


Assuntos
Antraz/prevenção & controle , Varíola/prevenção & controle , Vacinas Combinadas/imunologia , Vaccinia virus/imunologia , Adjuvantes Imunológicos , Animais , Anticorpos Antibacterianos/sangue , Anticorpos Antivirais/sangue , Bacillus anthracis/genética , Camundongos , Vacinas Atenuadas/genética , Vacinas Atenuadas/imunologia , Vacinas Combinadas/normas , Vacinas Sintéticas/imunologia , Vaccinia virus/genética
2.
J Vet Sci ; 20(1): 87-90, 2019 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-30481987

RESUMO

Sequence type (ST) 33 of Shiga toxin-producing Escherichia coli (STEC) strain O91:H14 has been proposed as a potential domestic clone of STEC in Korea because of its high prevalence among human patients with mild diarrhea or asymptomatic carriers. Herein, the clonal diversity of 17 STEC O91:H14 isolates of ST33 during 2003 to 2014 was analyzed by pulsed-field gel electrophoresis, including 14 isolates from human patients and 3 from retail meats. Their virulence characteristics, acid resistance, and antimicrobial susceptibility were also determined. Our results showed that all isolates were clustered mainly into three different pulsotypes and were likely low pathogenic without antimicrobial resistance.


Assuntos
Infecções por Escherichia coli/epidemiologia , Carne Vermelha/microbiologia , Toxinas Shiga/genética , Escherichia coli Shiga Toxigênica/genética , Escherichia coli Shiga Toxigênica/patogenicidade , Virulência/genética , Infecções por Escherichia coli/microbiologia , Microbiologia de Alimentos , Humanos , Testes de Sensibilidade Microbiana , Epidemiologia Molecular , Prevalência , República da Coreia/epidemiologia
3.
Genome Announc ; 6(17)2018 Apr 26.
Artigo em Inglês | MEDLINE | ID: mdl-29700157

RESUMO

We report here the draft genome sequence of Burkholderia pseudomallei H0901. This strain was isolated in 2003 from the first melioidosis patient in South Korea.

4.
Genome Announc ; 4(3)2016 Jun 16.
Artigo em Inglês | MEDLINE | ID: mdl-27313284

RESUMO

We report here the draft genome sequences of four vancomycin-intermediate Staphylococcus aureus (VISA) strains from South Korean hospitals participating in a nationwide laboratory surveillance program for vancomycin-intermediate and vancomycin-resistant Staphylococcus aureus All strains harbor mutations in the walKR, graSR, and/or rpoB genes that are known frequently mutated determinants of VISA.

5.
Diagn Microbiol Infect Dis ; 80(2): 148-50, 2014 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-25139845

RESUMO

Of 18 vanA-positive vancomycin-susceptible Enterococcus faecium isolates, vanRS in the vanA cluster was detected in all isolates, while vanHAX was detected in only 2 isolates. Following exposure to glycopeptides, 22.2% of vancomycin-susceptible E. faecium (VSE) converted into vancomycin-resistant E. faecium. The vanA cluster of the revertant mutant was transferred to the VSE isolates.


Assuntos
Antibacterianos/farmacologia , Proteínas de Bactérias/genética , Carbono-Oxigênio Ligases/genética , Enterococcus faecium/efeitos dos fármacos , Enterococcus faecium/genética , Resistência a Vancomicina , Vancomicina/farmacologia , Enterococcus faecium/isolamento & purificação , Genes Bacterianos , Infecções por Bactérias Gram-Positivas/microbiologia , Humanos , Família Multigênica
6.
J Korean Med Sci ; 29(3): 328-33, 2014 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-24616579

RESUMO

Pertussis is a representative vaccine-preventable disease. However, there have been recent outbreaks in countries where even higher vaccination against the disease. One reason is the emergence of antigenic variants, which are different to vaccine type. In Korea, reported cases have rapidly increased since 2009. Therefore, we analyzed genotype of strains isolated in 2011-2012 by multilocus sequence typing method. As expected, the genotype profiles of tested genes dramatically changed. The major sequence type changed from ST1 to ST2, and new sequence type (ST8) appeared. In the minimum spanning tree, recent isolates belonging to the ACC-I-ST3 subgroup were detected that were composed of ST2, ST3, and ST6. In particular, the ST2 frequency increased to 81%. The novel ST8 was linked to the increased frequency of ST2. In addition, toxic strains carrying the ptxP3 promoter type were confirmed. This ptxP3 type emerged from 2009 and its frequency had increased to 100% in 2012. Based on these results, it can be inferred that the genotypic changes in the currently circulating strains are strongly associated with the recent increasing of pertussis in Korea. Therefore, the surveillance system should be strengthened, and genetic characterization of the isolates should be expanded to the whole genome sequence level.


Assuntos
Variação Antigênica , Antígenos/genética , Bordetella pertussis/genética , Bordetella pertussis/metabolismo , Coqueluche/microbiologia , Antígenos/imunologia , Antígenos/metabolismo , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Bordetella pertussis/isolamento & purificação , Genes Bacterianos , Genótipo , Humanos , Toxina Pertussis/genética , Toxina Pertussis/metabolismo , Regiões Promotoras Genéticas , República da Coreia , Análise de Sequência de DNA , Coqueluche/imunologia , Coqueluche/patologia
7.
J Med Microbiol ; 62(Pt 8): 1204-1210, 2013 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-23657529

RESUMO

We investigated changes in serotypes and antimicrobial susceptibilities among 386 isolates of invasive Streptococcus pneumoniae collected from numerous hospitals in Korea from 1996 to 2008. Serotypes 19F (9.8 %), 23F (8.3 %), 19A (7.8 %), 6A (7.5 %), 3 (7.3 %), 9V (6.5 %), 6B (6.2 %), 14 (4.9 %), 1 (3.9 %), 11A (3.9 %) and 4 (3.1 %) represented 69.2 % of all isolates. While the overall proportion of PCV7 serotypes was stable over time, we observed modest decreases in children <5 years old and in adults ≥65 years old between 1996-1999 and 2007-2008. An increased prevalence of non-PCV7 serotypes in these age groups was primarily attributable to an increase in serotypes 3, 6A and 19A. Most invasive S. pneumoniae isolates showed high resistance rates to erythromycin (74.9 %), tetracycline (71.1 %) and clindamycin (61.7 %). Between 1996-2003 and 2004-2008, non-susceptibility rates to cefotaxime and multi-drugs (three or more classes) in PCV7 serotypes showed a declining trend, while in non-PCV7 serotypes there was an increasing trend. Non-PCV7 serotypes 6A and 19A, which mostly exhibited multidrug-resistant phenotypes (69.0 % and 76.7 % respectively), increased between 1996-2003 and 2004-2008. Although PCV7 was introduced in Korea in November 2003, pneumococcal vaccination has not been included in the national child vaccination programme. Our results provide details of serotype occurrence that will be useful when adoption of universal pneumococcal vaccination in Korea is being considered.


Assuntos
Antibacterianos/farmacologia , Infecções Pneumocócicas/epidemiologia , Infecções Pneumocócicas/microbiologia , Streptococcus pneumoniae/classificação , Streptococcus pneumoniae/efeitos dos fármacos , Adolescente , Adulto , Distribuição por Idade , Idoso , Idoso de 80 Anos ou mais , Criança , Pré-Escolar , Farmacorresistência Bacteriana Múltipla , Feminino , Humanos , Lactente , Masculino , Testes de Sensibilidade Microbiana , Pessoa de Meia-Idade , Prevalência , República da Coreia/epidemiologia , Sorotipagem , Streptococcus pneumoniae/isolamento & purificação , Adulto Jovem
8.
Osong Public Health Res Perspect ; 4(6): 293-300, 2013 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-24524017

RESUMO

OBJECTIVES: The proteomic analysis of voriconazole resistant Candida glabrata strain has not yet been investigated. In this study, differentially expressed proteins of intracellular and membrane fraction from voriconazole-susceptible, susceptible dose-dependent (S-DD), resistant C. glabrata strains were compared with each other and several proteins were identified. METHODS: The proteins of intracellular and membrane were isolated by disrupting cells with glass bead and centrifugation from voriconazole susceptible, S-DD, and resistant C. glabrata strains. The abundance of expressed proteins was compared using two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis and proteins showing continuous twofold or more increase or reduction of expression in resistant strains compared to susceptible and S-DD strain were analyzed by liquid chromatography/mass spectrometry-mass spectrometry method. RESULTS: Of 34 intracellular proteins, 15 proteins showed expression increase or reduction (twofold or more). The identified proteins included regulation, energy production, carbohydrate transport, amino acid transport, and various metabolism related proteins. The increase of expression of heat shock protein 70 was found. Among membrane proteins, 12, 31 proteins showed expression increase or decrease in the order of susceptible, S-DD, and resistant strains. This expression included carbohydrate metabolism, amino acid synthesis, and response to stress-related proteins. In membrane fractions, the change of expression of 10 heat shock proteins was observed, and 9 heat shock protein 70 (Hsp70) showed the reduction of expression. CONCLUSION: The expression of Hsp70 protein in membrane fraction is related to voriconazole resistant C. glabrata strains.

9.
J Med Microbiol ; 61(Pt 5): 678-685, 2012 May.
Artigo em Inglês | MEDLINE | ID: mdl-22282460

RESUMO

A longitudinal analysis was carried out of the colonization by four potential respiratory pathogens - Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis and Staphylococcus aureus - in 165 healthy children (aged 3-7 years) attending three kindergartens and 417 healthy children (aged 7-10 years) attending an elementary school in Seoul, Korea, by four consecutive examinations over 1 year. The prevalence of nasal carriers of one or more of four bacteria was found to be higher in younger children (≤7 years) (mean 68.6%) than that in older children (mean 46.8%). The mean rates of nasal carriage of Strep. pneumoniae, H. influenzae, M. catarrhalis and Staph. aureus were 16.8, 18.9, 20.2 and 18.2%, respectively. Colonization by Strep. pneumoniae, H. influenzae and M. catarrhalis was higher in pre-school children (28.6, 32.4 and 35.0%, respectively) than in school children (12.2, 13.6 and 14.3%, respectively). Carriage trends differed with age, with Strep. pneumoniae, H. influenzae and M. catarrhalis colonization decreasing with age but Staph. aureus colonization increasing. Positive associations of co-occurrence between Strep. pneumoniae, H. influenzae and M. catarrhalis were evident, with a significant negative association evident between Staph. aureus and the other three bacteria. A better understanding of the colonization and interaction of potential respiratory pathogens may be important for predicting changes in bacterial ecology and for designing control strategies that target bacterial colonization in upper respiratory tract infections.


Assuntos
Bactérias/classificação , Bactérias/isolamento & purificação , Infecções Bacterianas/epidemiologia , Infecções Bacterianas/microbiologia , Portador Sadio/epidemiologia , Portador Sadio/microbiologia , Nariz/microbiologia , Criança , Creches , Pré-Escolar , Feminino , Humanos , Estudos Longitudinais , Prevalência , República da Coreia/epidemiologia , Instituições Acadêmicas
10.
Osong Public Health Res Perspect ; 2(2): 115-26, 2011 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-24159461

RESUMO

OBJECTIVES: To confirm genotype diversities of clinical isolates of Bordetella pertussis and to evaluate the risk of pertussis outbreak in Korea. METHODS: Seven housekeeping genes and 10 antigenic determinant genes from clinical B. pertussis isolates were analyzed by Multilocus sequence typing (MLST). RESULTS: More variant pattern was observed in antigenic determinant genes. Especially, PtxS1 gene was the most variant gene; five genotypes were observed from eight global genotypes. In the bacterial type, the number of observed sequence types in the isolates was seven and the most frequent form was type 1 (79.6%). This major sequence type also showed a time-dependent transition pattern. Older isolates (1968 and 1975) showed type 1 and 6 in housekeeping genes and antigenic determinant genes, respectively. However, these were changed to type 2 and 1 in isolates 1999-2008. This transition was mainly attributed to genotype change of PtxS1 and Fim3 gene; the tendency of genotype change was to avoid vaccine-derived genotype. In addition, there was second transition in 2009. In this period, only the sequence type of antigenic determinant genes was changed to type 2. Based Upon Related Sequence Types (BURST) analysis confirmed that there were two clonal complexes (ACCI and ACCII) in the Korean isolates. Moreover, the recently increased sequence type was revealed as AST2 derived from AST 3 in ACCI. CONCLUSIONS: Genotype changes in Korean distributing strains are still progressing and there was a specific driving force in antigenic determinant genes. Therefore continuous surveillance of genotype change of the distributing strains should be performed to confirm interrelationship of genotype change with vaccine immunity.

11.
J Microbiol ; 48(5): 547-53, 2010 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-21046330

RESUMO

The molecular typing of 202 Legionella pneumophila sg 1 isolates obtained from environmental water sources and clinical specimens from 1985 to 2007 was conducted using pulsed-field gel electrophoresis (PFGE). In this study, a total of 212 isolates were grouped into 35 different PFGE types and Type 1 was the predominant type, accounting for 28.7% in PFGE types. Type 1 and Type 8 were observed continuously from 1985 to 2007. In the analysis of the distribution of PFGE types in six geographic regions (Seoul-Incheon, Gangwon, Chungcheong, Gyeongsang, Jeolla, and Jeju), Type 1 was predominant throughout four regions except for Jeolla and Jeju, and Type 6 was observed in four regions except two regions (Gangwon and Jeju). Six clinical isolates belonged to PFGE Type 1, Type 6, Type 9, and Type 15. Type 1 among these types, was isolated from 3 patients with confirmed nosocomial infection at the hospital and Type 6, Type 9, and Type 15 were isolated 3 patients with suspected community-acquired infection. Type R, PFGE pattern of L. pneumophila sg 1 (ATCC 33152, Philadelphia-1), was not observed in the isolates evaluated in this study. Therefore, our results suggest that PFGE Type 1 was very prevalent in the environmental and clinical isolates in Korea. Type 1 was distributed continuously for many years throughout Korea.


Assuntos
Técnicas de Tipagem Bacteriana , DNA Bacteriano/genética , Eletroforese em Gel de Campo Pulsado , Legionella pneumophila/classificação , Legionella pneumophila/genética , Doença dos Legionários/microbiologia , Microbiologia da Água , Análise por Conglomerados , Impressões Digitais de DNA , Genótipo , Geografia , Humanos , Coreia (Geográfico) , Legionella pneumophila/isolamento & purificação , Epidemiologia Molecular
12.
Appl Environ Microbiol ; 76(19): 6547-54, 2010 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-20693456

RESUMO

A total of 560 Legionella species were isolated from environmental water sources from public facilities from June to September 2008 throughout South Korea. The distribution of Legionella isolates was investigated according to geographical region, facility type, and sample type. The genetic diversity of 104 isolates of Legionella pneumophila serogroup 1 (sg 1) was analyzed by sequence-based typing (SBT). L. pneumophila was distributed broadly throughout Korea, accounting for 85.0% of the isolates, and L. pneumophila sg 1 predominated in all of the public facilities except for the springs. Legionella anisa and Legionella bozemanii predominated among non-L. pneumophila species (48.1% and 21.0%, respectively). The second most dominant strain differed depending on the facility type: L. anisa was the second most dominant strain in the buildings (10.8%), L. pneumophila sg 5 in public baths (21.6%), L. pneumophila sg 6 in factories (12.0%), and L. pneumophila sg 7 in hospitals (13.1%). In the SBT analysis, 104 L. pneumophila sg 1 isolates were differentiated into 26 sequence types (STs) and categorized into 3 clonal groups (CGs) and 10 singleton STs via the eBURST V3 program. ST1, a potential founder of major CG1, was commonly distributed (48.1%). The dominant ST in hot water was ST-K1 (7, 12, 17, 3, 35, 11, 11), which was designated in this study (36.1%). The second most dominant strain differed depending on the type of facility from which the samples were obtained. The unique allelic profile of ST-K1, obtained from hot water, was not found in the European Working Group for Legionella Infections (EWGLI) SBT database.


Assuntos
Variação Genética , Legionella/classificação , Legionella/genética , Logradouros Públicos , Microbiologia da Água , Técnicas de Tipagem Bacteriana , Impressões Digitais de DNA , DNA Bacteriano/genética , Geografia , Legionella/isolamento & purificação , Prevalência , República da Coreia
13.
J Microbiol Biotechnol ; 19(3): 323-30, 2009 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-19349759

RESUMO

Nontuberculous mycobacteria (NTM) are a major cause of opportunistic infections in immunocompromised patients, making the reliable and rapid identification of NTM to the species level very important for the treatment of such patients. Therefore, this study evaluated the usefulness of the novel target genes tuf and tmRNA for the identification of NTM to the species level, using a PCRrestriction fragment length polymorphism analysis (PRA). A total of 44 reference strains and 17 clinical isolates of the genus Mycobacterium were used. The 741 bp or 744 bp tuf genes were amplified, restricted with two restriction enzymes (HaeIII/MboI), and sequenced. The tuf gene-PRA patterns were compared with those for the tmRNA (AvaII), hsp65 (HaeIII/HphI), rpoB (MspI/HaeIII), and 16S rRNA (HaeIII) genes. For the reference strains, the tuf gene-PRA yielded 43 HaeIII patterns, of which 35 (81.4%) showed unique patterns on the species level, whereas the tmRNA, hsp65, rpoB, and 16S rRNA-PRAs only showed 10 (23.3%), 32 (74.4%), 19 (44.2%), and 3 (7%) unique patterns after single digestion, respectively. The tuf gene-PRA produced a clear distinction between closely related NTM species, such as M. abscessus (557-84- 58) and M. chelonae (477-84-80-58), and M. kansasii (141- 136-80-63-58-54-51) and M. gastri (141-136-117-80-58-51). No difference was observed between the tuf-PRA patterns for the reference strains and clinical isolates. Thus, a diagnostic algorithm using a tuf gene-targeting PRA is a promising tool with more advantages than the previously used hsp65, rpoB, and 16S rRNA genes for the identification of NTM to the species level.


Assuntos
Algoritmos , Infecções por Mycobacterium/diagnóstico , Mycobacterium/genética , Mycobacterium/isolamento & purificação , Fator Tu de Elongação de Peptídeos/genética , Análise do Polimorfismo de Comprimento de Fragmentos Amplificados , Proteínas de Bactérias/genética , Técnicas de Tipagem Bacteriana/métodos , Sequência de Bases , Chaperonina 60 , Chaperoninas/genética , DNA Bacteriano/isolamento & purificação , Humanos , Dados de Sequência Molecular , Mycobacterium/classificação , Infecções por Mycobacterium/microbiologia , RNA Bacteriano/genética , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Especificidade da Espécie
14.
J Microbiol ; 46(6): 608-14, 2008 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-19107388

RESUMO

Nontuberculous mycobacteria (NTM) are ubiquitous in the environment and can cause nosocomial infections in immunocompromised patients. Recently the presence of NTM in public drinking water and hospital water distribution systems has been reported. Their ability to form biofilms and their resistance to chlorine both contribute to their survival and colonization in water distribution systems. Here we analyzed thirty-two hospital tap water samples that were collected from different locations in three hospitals so as to evaluate the prevalence of NTM species. The water samples were concentrated by membrane filtration and then eluted with sterilized water following sonication. Two-step direct PCR targeting the rpoB gene, restriction fragment length polymorphism (RFLP) using the MspI restriction enzyme, and sequence analysis were performed for identification of NTM to the species level. The sequences of each PCR product were analyzed using BLASTN. Seven samples (7/32, 21.9%) were positive for NTM as determined by nested-PCR. The PCR-RFLP results indicated five different patterns among the seven positive PCR samples. The water-born NTM were identified, including M. peregrinum, M. chelonae (2 cases), M. abscessus, M. gordonae (2 cases), and Mycobacterium sp. JLS. The direct two-step PCR-RFLP method targeting the rpoB gene was effective for the detection and the differentiation of NTM species from hospital tap water.


Assuntos
RNA Polimerases Dirigidas por DNA/genética , Água Doce/microbiologia , Hospitais , Mycobacterium/classificação , Reação em Cadeia da Polimerase/métodos , Polimorfismo de Fragmento de Restrição , Abastecimento de Água , Técnicas de Tipagem Bacteriana , Desoxirribonuclease HpaII , Coreia (Geográfico) , Mycobacterium/genética , Mycobacterium/isolamento & purificação , Especificidade da Espécie
15.
Jpn J Infect Dis ; 59(3): 192-4, 2006 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-16785703

RESUMO

Six hundred fifteen isolates of Streptococcus pyogenes were collected over a 6-year period from patients with pharyngitis in Korea. All isolates were characterized in terms of their antibiotic resistance, the phenotypes of erythromycin resistance, the frequencies of erm(B), erm(A), and mef(A) genes, and the emm genotype. The prevalent emm genotypes were emm12 and emm4. Moreover, the emm12 genotype was found to be the most resistant strain to erythromycin. Among the 126 strains demonstrating resistance to erythromycin, those with erm(B) were the most prevalent, accounting for 64.3% of the total. In summary, it is suggested that the S. pyogenes pathogen isolated from pharyngitis patients in Korea developed resistant gene acquisition, as well as a resistant phenotype, according to the annual prevailing emm type. It is also suggested that the emm genotype distribution of erythromycin-resistant strains is correlated to the acquisition of resistant genes.


Assuntos
Antibacterianos/farmacologia , Eritromicina/farmacologia , Faringite/microbiologia , Streptococcus pyogenes/efeitos dos fármacos , Farmacorresistência Bacteriana , Humanos , Coreia (Geográfico) , Streptococcus pyogenes/isolamento & purificação
16.
FEMS Immunol Med Microbiol ; 45(2): 341-7, 2005 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-16019195

RESUMO

Current human anthrax vaccines available in the United States and Europe consist of alum-precipitated supernatant material from cultures of a toxigenic, nonencapsulated strain of Bacillus anthracis. The major component of human anthrax vaccine that confers protection is protective antigen (PA). A second-generation human vaccine using the recombinant PA (rPA) is being developed. In this study, to prevent the toxicity and the degradation of the native rPA by proteases, we constructed two PA variants, delPA (163-168) and delPA (313-314), that lack trypsin (S(163)-R(164)-K(165)-K(166)-R(167)-S(168)) or chymotrypsin cleavage sequence (F(313)-F(314)), respectively. These proteins were expressed in Bacillus brevis 47-5Q. The delPAs were fractionated from the culture supernatant of B. brevis by ammonium sulfate at 70% saturation, followed by anion exchange chromatography on a Hitrap Q, Hiload 16/60 superdex 200 gel filtration column and phenyl sepharose hydrophobic interaction column. In accordance with previous reports, both delPA proteins combined with lethal factor protein did not show any cytotoxicity on J774A.1 cells. The delPA (163-168) and delPA (313-314) formulated either in Rehydragel HPA or MPL-TDM-CWS (Ribi-Trimix), elicited a comparable amount of anti-PA and neutralizing antibodies to those of native rPA in guinea pigs, and confers full protection of guinea pigs from 50xLD50 of fully virulent B. anthracis spore challenges. Ribi-Trimix was significantly more effective in inducing anti-PA and neutralizing antibodies than Rehydragel HPA. These results indicate the possibility of delPA (163-168) and delPA (313-314) proteins being developed into nontoxic, effective and stable recombinant vaccine candidates.


Assuntos
Antígenos de Bactérias/genética , Bacillus anthracis/genética , Bacillus anthracis/imunologia , Toxinas Bacterianas/genética , Sequência de Aminoácidos , Animais , Vacinas contra Antraz/genética , Vacinas contra Antraz/imunologia , Vacinas contra Antraz/isolamento & purificação , Vacinas contra Antraz/toxicidade , Anticorpos Antibacterianos/biossíntese , Antígenos de Bactérias/imunologia , Antígenos de Bactérias/isolamento & purificação , Antígenos de Bactérias/toxicidade , Bacillus/genética , Toxinas Bacterianas/imunologia , Toxinas Bacterianas/isolamento & purificação , Toxinas Bacterianas/toxicidade , Sequência de Bases , Sítios de Ligação/genética , Linhagem Celular , Quimotripsina , DNA Bacteriano/genética , Feminino , Genes Bacterianos , Cobaias , Humanos , Camundongos , Mutação , Testes de Neutralização , Proteínas Recombinantes/genética , Proteínas Recombinantes/imunologia , Proteínas Recombinantes/isolamento & purificação , Proteínas Recombinantes/toxicidade , Deleção de Sequência , Tripsina
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