1.
Sheng Wu Gong Cheng Xue Bao
; 18(1): 106-8, 2002 Jan.
Artigo
em Chinês
| MEDLINE
| ID: mdl-11977587
RESUMO
According to the reported sequence of Buthus martensii Karsch scorpion toxin gene (BmK IT3), we synthesized two primers, which were complementary in a region. By the means of PCR, we got the gene. The gene was fused in expression vector pET-28a, which gave rise to a recombinant plasmid pET(IT3R). Then it was transformed into E. coli BL21 (DE3). With IPTG induction, the gene was efficiently expressed. And the fusion product was soluble.