Physiological and transcriptome analysis reveals that prohexadione-calcium promotes rice seedling's development under salt stress by regulating antioxidant processes and photosynthesis.

Prohexadione-calcium (Pro-Ca) has been proved to play an important role in releasing abiotic stress in plants. However, there is still a lack of research on the mechanism of Pro-Ca alleviating salt stress in rice. To explore the protective effects of Pro-Ca on rice seedlings under salt stress, we investigated the effect of exogenous Pro-Ca on rice seedling under salt stress by conducting the following three treatment experiments: CK (control), S (50 mmol·L-1 NaCl saline solution) and S + Pro-Ca (50 mmol·L-1 NaCl saline solution + 100 mg·L-1 Pro-Ca). The results indicated that Pro-Ca modulated the expression of antioxidant enzyme-related genes (such as SOD2, PXMP2, MPV17, E1.11.1.7). Spraying Pro-Ca under salt stress significantly increased in ascorbate peroxidase, superoxide dismutase, and peroxidase activity by 84.2%, 75.2%, and 3.5% as compared to the salt treatment, as demonstrated by an example of a 24-hour treatment. Malondialdehyde level in Pro-Ca was also dramatically decreased by 5.8%. Moreover, spraying Pro-Ca under salt stress regulated the expression of photosynthesis genes (such as PsbS, PsbD) and chlorophyll metabolism genes (heml, PPD). Compared to salt stress treatment, spraying Pro-Ca under salt stress significantly increased in net photosynthetic rate by 167.2%. In addition, when rice shoots were sprayed with Pro-Ca under salt stress, the Na+ concentration was considerably reduced by 17.1% compared to salt treatment. In conclusion, Pro-Ca regulates antioxidant mechanisms and photosynthesis to aid in the growth of rice seedlings under salt stress.

Exogenous uniconazole enhances tolerance to chilling stress in mung beans (Vigna radiata L.) through cross talk among photosynthesis, antioxidant system, sucrose metabolism, and hormones.

To monitor the role of exogenous uniconazole in mitigating chilling stress, this study investigated the effect of foliar spraying of 50 mg L-1 uniconazole on the chilling (15 °C) tolerance of mung beans at the flowering stage. The results showed that uniconazole significantly enhanced the reactive oxygen species (ROS) scavenging ability of mung beans by increasing the superoxide dismutase (SOD), peroxidase (POD), ascorbate peroxidase (APX), glutathione reductase (GR) activities, the contents of ascorbic acid (AsA) and glutathione (GSH), and the transcription levels of SOD and POD under chilling stress. The uniconazole applications also drastically increased the net photosynthetic rate (Pn), maximum net photosynthetic rate (Pnmax), maximum quantum yield of PSII (Fv/Fm), and the expression levels of the corresponding photosynthetic genes PsbO, PsbP, PsbQ, PsbY, and Psb28. This, in turn, resulted in a higher sucrose content. Meanwhile, uniconazole increased the indole-3-acetic acid (IAA) content but reduced the gibberellin A3 (GA3) content under chilling stress. During the recovery period, the photosynthetic parameters and ROS of plants receiving uniconazole recovered faster, and the antioxidant activity and non-antioxidant contents were higher than in chilling-treated plants. Additionally, chilling stress markedly reduced the pod number per plant, grain number per plant, and 100-seed weight, whereas uniconazole significantly increased the grain weight per plant by 53.47% compared to the chilling treatment. These results strongly suggest that uniconazole can effectively protect mung beans from chilling stress damage by protecting the photosynthetic machinery and enhancing the antioxidant capacity to quench excessive ROS caused by chilling stress. These effects are closely relevant to chilling tolerance enhancement and yield improvement in mung beans.

Prohexadione-calcium alleviates saline-alkali stress in soybean seedlings by improving the photosynthesis and up-regulating antioxidant defense.

Soil salinization seriously restricts the growth and yield of soybeans. However, little information is available on the early growth stages of soybeans which are subjected to the gibberellin biosynthesis inhibitor, prohexadione-calcium (Pro-Ca). This study aimed to investigate the effects of exogenous Pro-Ca on saline-alkali stress-induced damages to photosynthesis and antioxidant defenses in soybean (Glycine max L.) seedlings. At the V3 growth stage, salt-tolerant genotype Hefeng 50 (HF50) and salt-sensitive genotype Kenfeng 16 (KF16) were subjected to 110 mmol L-1 mixed saline-alkali stress respectively, and then 100 mg L-1 Pro-Ca was sprayed on the leaves. Our results showed that saline-alkali stress accelerated the degradation of thylakoids, inhibited chlorophyll synthesis, reduced shoot dry weight, electron transfer rate (ETR), and peroxidase (POD) activity, the concentration of ascorbic acid (AsA) and soluble sugar, but enhanced the concentration of proline, hydrogen peroxide (H2O2) and the rate of superoxide radical (O2∙-) generation. Additionally, saline-alkali stress induced a lower decrease of the net photosynthetic rate (Pn), potential activity of PSII (Fv/F0), and maximum quantum yield of PSII (Fv/Fm) in salt-tolerant HF50 than in salt-sensitive KF16. Nevertheless, foliar spraying of exogenous Pro-Ca increased the chlorophyll content, Pn, Fv/F0, and Fv/Fm. These results were more prominent when Pro-Ca was applied to KF16 under saline-alkali conditions. Furthermore, exogenous application of Pro-Ca retarded the degradation of thylakoids, increased the ETR and the accumulation of AsA, soluble sugar, and proline, activated the activities of superoxide dismutase (SOD), catalase (CAT), and POD, and decreased the concentration of malondialdehyde (MDA), electrolyte leakage (EL), O2∙-, and H2O2. These results indicated that Pro-Ca could effectively protect soybean seedlings against damage from saline-alkali stress by regulating seedling phenotype, photosynthetic apparatus, antioxidant defense, and osmoregulation.