RESUMO
In this work, we proposed an immobilization scheme targeting Escherichia coli bacterial cells onto gold surface utilizing polypeptide RGD's binding specificity to gold particles. Adsorption kinetics of extracellular Herring Sperm DNA on E. coli was then studied using electrochemical methods along with surface plasmon resonance spectroscopy through this immobilization scheme. The adsorption equilibrium constants of DNA adsorbing to E. coli from electrochemical method and surface plasmon resonance spectroscopy, were (5.596±0.462)×105 L mol(-1) and (1.24±0.361)×105 L mol(-1), accordingly. Importantly, this is the first study that used an electrochemical method to express the adsorptive action of DNA by E. coli.
Assuntos
DNA/metabolismo , Eletroquímica/métodos , Escherichia coli/metabolismo , Adsorção , Animais , Espectroscopia Dielétrica , Eletrodos , Peixes , Ouro/química , Concentração de Íons de Hidrogênio , Cinética , Masculino , Microscopia de Força Atômica , Espermatozoides/metabolismo , Ressonância de Plasmônio de Superfície , Propriedades de SuperfícieRESUMO
Honokiol has shown the ability to induce the apoptosis of several different cancer cell lines. Considering that mitochondria are involved in apoptosis, the aim of the present work was to investigate the effects of honokiol on mitochondria. The effects of honokiol on the permeability of H⺠and Kâº, membrane potential, membrane fluidity, respiration and swelling of mitochondria isolated from the rat liver were assessed. The results show that honokiol can significantly induce mitochondrial swelling, decrease membrane potential and affect the respiration of mitochondria. Meanwhile, honokiol does not have a direct effect on the mitochondrial permeability transition pore.
