RESUMO
OBJECTIVE: To investigate the feasibility and technology for purifying Ganodermna lucidum polysaccharide (GLP) by ultrafiltration. METHODS: A spire roll ultrafiltration unit was selected to purify GLP. RESULTS: Purity of 73.5% and recovery ratio of 69.4% for GLP were obtained by using a membrane with cut-off molecular mass of 6000, while operating temperature, pressure and time were 45-50 degrees C, 0.6 MPa and 45 min, respectively. CONCLUSION: Ultrafiltration is one of the new feasible methods for the purification of GLP.
Assuntos
Antineoplásicos/isolamento & purificação , Polissacarídeos/isolamento & purificação , Reishi/química , Ultrafiltração/métodos , Antineoplásicos/química , Estudos de Viabilidade , Peso Molecular , Polissacarídeos/química , Pressão , Espectrofotometria Ultravioleta , Esporos Fúngicos/química , Temperatura , Fatores de Tempo , Ultrafiltração/instrumentaçãoRESUMO
OBJECTIVE: To isolate and purify Chlorogenic acid from Eucommia ulmoides leaves by ultrafiltration technique. METHODS: Chlorogenic acid transferring ratio was investigated by temperature, pressure, time, cut-off molecular mass. RESULTS: The optimal conditions for the ultrafiltration technique were finally determined. It was found that, for the membrane with a cut-off molecular mass of 20000, when the temperature, pressure, time were respectively 40 degrees C, 0.36 MPa, 20 minutes and adding 24L water, yield of Chlorogenic acid was 99.38%. CONCLUSION: High Chlorogenic acid transfering ratio can be gotten by ultrafiltration technique, which is helpful to the further isolation and purification of Chlorogenic acid in the subsequent process.
