RESUMO
Two new compounds, 5-O-methyl-4-desmethyl-myricanol (1) and 6-formyl-5-isopropyl-3-hydroxymethyl-7-methyl-1H-indene (2), were isolated from the leaves of Micromelum integerrimum. Their structures were determined by spectroscopic methods. Additionally, compound 1 could stimulate the growth of NIH3T3 cells and promote cell migration. Compound 1 might exert its effects through increasing the protein expression of connective tissue growth factor.
Assuntos
Diarileptanoides/química , Indenos/química , Folhas de Planta/química , Rutaceae/química , Animais , Movimento Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Camundongos , Estrutura Molecular , Células NIH 3T3RESUMO
We sought to compare the preventive effects of mitomycin-C(MMC) and chitosan on intraarticular adhesion after knee surgery in rabbits. For this purpose, 48 New-Zealand rabbits were randomly and equally divided into MMC, chitosan, and control groups. Approximately 10 × 10 mm(2) of the cortical bone was removed from both sides of left femoral condyle and the cancellous bone underneath was exposed. The decorticated areas were topically treated with MMC and chitosan while control group was treated with physiological saline. The lower left limb was fixed in flexed position with Kirschner-wire for 4 weeks postoperatively. After 4 weeks, gross and histopathological examination, biochemical analysis, and fibroblast counts were performed on knee intraarticular adhesion in each group. The data show mild membrane-like fibrous intraarticular adhesion, presented in loose, in MMC group. There was moderate intraarticular adhesion in chitosan group while in controls; there was large-size compact fibrous tissue adhesion. Hydroxyproline contents and fibroblast quantity of MMC and chitosan groups were lower (P < 0.05) than that of control group. We, therefore, concluded that MMC and chitosan could prevent intraarticular adhesion of the knee in rabbits by inhibiting fibroblast proliferation and reducing collagenous fiber formation while MMC had a better preventive effect than that of chitosan.
Assuntos
Alquilantes/farmacologia , Quitosana/farmacologia , Cicatriz/prevenção & controle , Articulação do Joelho/efeitos dos fármacos , Articulação do Joelho/cirurgia , Mitomicina/farmacologia , Aderências Teciduais/prevenção & controle , Animais , Cicatriz/metabolismo , Cicatriz/patologia , Modelos Animais de Doenças , Fibroblastos/efeitos dos fármacos , Fibroblastos/metabolismo , Fibroblastos/patologia , Hidroxiprolina/metabolismo , Articulação do Joelho/metabolismo , Articulação do Joelho/patologia , Masculino , Coelhos , Aderências Teciduais/metabolismo , Aderências Teciduais/patologiaRESUMO
Mitomycin C (MMC) is known to inhibit fibroblast proliferation through suppressing DNA dependent RNA synthesis. Based on this knowledge, we illustrated the effect of MMC on inhibiting fibroblast collagen synthesis and reducing intraarticular fibrous adhesion in a rabbit model. Forty-eight New Zealand white rabbits were randomly divided into four groups. Approximately 10 x 10 mm squares of the cortical bone was removed from both sides of the femoral condyle, and the underneath cancellous bone was exposed. MMC in various concentrations or saline were then applied to the decorticated areas. The intraarticular adhesions were evaluated after four weeks by macroscopic evaluation, histological evaluation and biochemical analysis of hydroxyproline content. The results demonstrated that MMC could suppress the formation of intraarticular fibrous adhesion in a dose-dependent manner. The intraarticular adhesion score, the hydroxyproline content and the fibroblast number in 0.1mg/ml MMC group were significantly less than those in 0.05 mg/ml MMC group, 0.01 mg/ml MMC group and control group. However, dense adhesions were found in 0.01 mg/ml MMC group and control group. These results indicated that topical application of 0.1mg/ml MMC could reduce intraarticular adhesion through inhibiting fibroblast proliferation in rabbits.
