The mode of yearly biological cycle of malignant growth of hepatocellular carcinoma on computed tomography.

OBJECTIVE: To detect the mode of yearly biological cycle of malignant biological behavior of hepatocellular carcinoma (HCC). METHODS: Twenty-one kinds of CT signs reflecting various degrees of malignant biological behavior were determined. A total of 360 patients were collected by random sampling of 30 patients each month. CT signs of each patient were fitted in corresponding group of yearly cyclic data respectively by cosine curves and analysed in terms of sequence characteristics (cosinor). RESULTS: With a 95% confidence, 10 CT signs showed biological rhythm (P<0.05). The acrophase of CT features for highly-invasive growth concentrated between -60 degree and -120 degree from March to April. For low-invasive growth, however, the CT features were relatively low and concentrated between -180 degrees and -270 degrees from July to September. No acrophase was shown between -120 degrees and -180 degrees from May to June, and between -270 degrees and -330 degrees from October to November. CONCLUSION: Between CT signs of the highly-invasive and those of low-invasive growth of HCC, a sequential difference in biological cycles can be observed.

[Comparison of the yearly cycle cosine curve imitative parameters of the clinical features of liver cancer at different longitude and latitude].

OBJECTIVE: To explore whether there exists coincidence of the most appearing time of clinical features of liver cancer at different longitude and latitude, according to the law of field equation and the theory of warpage of space time by Einstein. METHODS: Three regions with different longitude and latitude were selected randomly and sampled. There were 36 items altogether, including 12 clinical items, which were used to imitate the yearly cycle cosine curve. The acorphases and the ratioes of amplitudes and means were compared to justifying whether they were in the same range. RESULTS: All the acorphases of 36 items appeared between -90.1degrees to -207.5 degrees (from april to july), existing in one third of the same range, in which 13 items occurred rhythmly (P<0.05). The image acorphases of liver cancer at the early and middle stage and gamma-glutamyl transpeptidase acorphase appeared between -98.5 degrees to -148.2 degrees (from april to may), in which 5 items occurred rhythmly (P<0.05). CONCLUSION: It is the same mode of the yearly biologcal cycle for liver cancer malignant growth within the most appearing time (from april to july). It will increase the detecting rate of liver cancer at the early and middle stage during this time (especially from april to may).

[Chronobiologic contrast of expression of p53, p16, and cyclin D1 in hepatocarcinoma cells].

OBJECTIVE: To contrast the feature of different periods of p53, p16, and cyclin D1 expression in cells of hepatocarcinoma. METHODS: Sixty hepatocarcinoma specimens were collected and divided into three periods in one yearly cycle with each period of 20 cases. Hepatocellular carcinoma was documented in all cases by pathomorphological classification. Edmondson-Steinet grading belonged to II~III stages. The expression of gene proteins of p53, p16, and cyclin D1 was determined by immunohistochemical assay (S-P method). The expressive intensity was analysed by the rank test. RESULTS: p16 expression showed significant difference of period (H=10.334, P<0. 05). Arpil-July was the high expressive period. CONCLUSIONS: p16 may take an important role in the chronobiologic mechanism of gene control of hepatocyte canceration and hepatocarcinoma cell growth.