High-Fat Diet Enhances the Liver Metastasis Potential of Colorectal Cancer through Microbiota Dysbiosis.

Obesity, metabolic changes, and intestinal microbiota disruption significantly affect tumorigenesis and metastasis in colorectal cancer (CRC). However, the relationships among these factors remain poorly understood. In this study, we found that a high-fat diet (HFD) promoted gut barrier dysfunction and inflammation in the colorectum and liver. We further investigated gut microbiota changes through 16S rRNA sequencing of faecal samples from HFD-fed rats and CRC hepatic metastasis patients and found an abundance of Desulfovibrio (DSV). DSV could also induce barrier dysfunction in the colorectum and inflammation in the colorectum and liver, suggesting that it contributes to the formation of a microenvironment conducive to CRC tumorigenesis and metastasis. These findings highlight that HFD-induced microbiota dysbiosis, especially DSV abundance, could promote CRC initiation and metastasis.

Geothermal Distribution Characteristics in the Qinshui Basin and Its Significance to the Production of Coalbed Methane.

Temperature significantly affects the storage and transport of coalbed methane (CBM). Studies of geothermal distribution characteristics are important for the exploration and exploitation of CBM. In this study, more than 150 heat flow temperature data from coalbed methane wells in the Qinshui Basin were analyzed to investigate the geothermal distribution and its controlling factors. The results show that the geothermal gradient of the no. 3 coal seam ranges from 0 to 3.7 °C/hm with an average of 1.6 °C/hm, and the terrestrial heat flow of the no. 3 coal reservoir ranges from 0.9 to 94.6 mW/m2 with an average of 41.5 mW/m2. The reservoir temperature shows high values in the central and northwest parts of the basin, while the east and west edges of the basin show negative geothermal anomalies. It is found that groundwater has significant effects on the geothermal distribution in the Qinshui Basin, and with the increase of the groundwater level, the geothermal gradient decreases linearly. In addition, the geothermal gradient and terrestrial heat flow first increase and then tend to be stable with the increase in value of the total dissolved substances. Besides, with an increase in floor elevation, the geothermal gradient first increases linearly and then decreases linearly, obtaining a maximum value at about 450 m (transition floor elevation). This phenomenon is the result of the balance between heat supplying and heat losing. The geothermal distribution characteristics in the Qinshui Basin determine the reservoir temperature of the coalbed methane, and in turn, the reservoir temperature affects the adsorption, desorption, and diffusion behaviors of coalbed methane in situ.

Efficient Tissue Culture Protocol for Magnolia lucida (Magnoliaceae) and Confirmation of Genetic Stability of the Regenerated Plants.

Magnolia lucida (Magnoliaceae) is classified as an endangered species by the International Union for Conservation of Nature. It has high commercial value owing to its attractive tree shape and flowers. We adopted an excellent genotype of M. lucida as the parent material and established a mini-cut orchard through grafting to provide trunk shoots explants over the long-term. Optimal sterilization was achieved using a combination of 75% ethanol for 30 s, one percent benzalkonium bromide for five minutes, and 0.1% mercuric chloride for five minutes. Modified Murashige and Skoog medium (ML) was the optimal medium for the growth of M. lucida. Addition of one mg/L of 6-benzyl adenine (BA) and 0.05 mg/L of α-naphthaleneacetic acid (NAA) to the medium increased the shoot induction rate to 95.56%, and the ML medium containing 0.4 mg/L BA and 0.04 mg/L NAA achieved the maximum multiplication rate (284.56%). Dark treatment for seven days, followed by continuous light treatment could better resolve the challenge of difficult rooting in M. lucida plants. Using random amplified polymorphic DNA and inter simple sequence repeat markers, we confirmed the genetic uniformity and stability of the regenerated plants. Our protocol should be helpful for the propagation and conservation of this endangered plant.

The Role of Aberrant Metabolism in Cancer: Insights Into the Interplay Between Cell Metabolic Reprogramming, Metabolic Syndrome, and Cancer.

Metabolic syndrome (MetS) is characterized by hyperglycemia, hypertension, dyslipidemia and abdominal obesity. Patients with MetS or other metabolic disorders are more susceptible to cancer development and recurrence and have a worse long-term prognosis. Moreover, the metabolic reprogramming observed in cancer cells has also been described as one of the new hallmarks of cancer. Thus, aberrant metabolism has been proposed as an important risk factor for cancer. Chronic inflammation, reactive oxygen species (ROS), and oncogenic signaling pathways are considered as main potential triggers. Considering the strong association between metabolism and cancer, metabolism-modulating drugs, including metformin and statins, as well as adopting a healthy lifestyle, have been extensively investigated as strategies to combat cancer. Furthermore, strategies that interfere with the metabolic rewiring of cells may also have potent anti-cancer effects. In this article, we provide a comprehensive review of current knowledge on the relationship between aberrant metabolism and cancer and discuss the potential use of metabolism-targeting strategy for the treatment of cancer.

Short-Term Temperature Response of Leaf Respiration in Different Subtropical Urban Tree Species.

Plant leaf respiration is one of the critical components of the carbon cycle in terrestrial ecosystems. To predict changes of carbon emissions from leaves to the atmosphere under a warming climate, it is, therefore, important to understand the thermodynamics of the temperature response of leaf respiration. In this study, we measured the short-term temperature response of leaf respiration from five different urban tree species in a subtropical region of southern China. We applied two models, including an empirical model (the Kavanau model) and a mechanistic model (Macromolecular Rate Theory, MMRT), to investigate the thermodynamic properties in different plant species. Both models are equivalent in fitting measurements of the temperature response of leaf respiration with no significant difference (p = 0.67) in model efficiency, while MMRT provides an easy way to determine the thermodynamic properties, i.e., enthalpy, entropy, and Gibbs free energy of activation, for plant respiration. We found a conserved temperature response in the five studied plant species, showing no difference in thermodynamic properties and the relative temperature sensitivity for different species at low temperatures (<42°C). However, divergent temperature response among species happened at high temperatures over 42°C, showing more than two-fold differences in relative respiration rate compared to that below 42°C, although the causes of the divergent temperature response remain unclear. Notably, the convergent temperature response at low temperatures could provide useful information for land surface models to improve predictions of climate change effects on plant respiration.

Mikania micrantha invasion enhances the carbon (C) transfer from plant to soil and mediates the soil C utilization through altering microbial community.

Exotic plant invasion alters the structure and coverage of terrestrial vegetation and affects the carbon (C) stocks in ecosystems. Previous studies have shown the increases in the C stocks with increasing invasive plants, but these results remain contentious. Soil microbial communities are usually altered by plant invasion, which potentially influences the C cycling underground. We hypothesized that the plant invasion-caused dynamic changes in soil microbes would lead to the corresponding change in soil C accumulation. Using greenhouse experiments we simulated different invader intensities through varying the relative abundance of invasive species Mikania micrantha and its co-occurring native species Paederia scandens. By analyzing 13C-phospholipid fatty acid we found the invasive M. micrantha assimilated more 13C and transferred faster the fixed 13C through different tissues to soils, as compared to native P. scandens. Soil microbial components, i.e., i15:0, 16:0, 10Me16:0, 18:1w9c and 18:2w6,9 were mainly using the photo-assimilated 13C. In addition, we found a hump-shaped relationship between soil net 13C accumulate rate and rhizosphere microbial biomass, indicating that the soil C accumulation may be either enhanced or reduced in invaded ecosystems, depending on microbe abundance.