RESUMO
The Taenia asiatica eggs pre-incubated with sodium hypochlorite solution for 4 min, 6 min and 8 mins were subcutaneously injected into mice with normal immune function(groups Al-A3 respectively, n=20 in each) and mice with immunosuppression (groups B1-B3, n=20 in each). All groups of mice began to show body discomfort on day 5 after infection and develop lumps on the back about on day 15. In groups Al-A3, animal death occurred during days 7-15, with a same survival rate of 95.0%(19/20) and infection rate of 89.4%(17/19), 73.6%(14/19) and 47.3%(9/19) respectively. In groups B1-B3, animal death occurred during days 7-50, with survival rate of 60%(13/20), 55%(11/20)and 55%(11/20) and infection rate of 76.9% (10/13), 54.5% (6/11) and 45.4% (5/11) respectively. After the scolex of cysticercus was evaginated with 15% pig bile, four suckers, an apparent rostellum and two distinct hook-like puncta structures were seen. These results indicate that mice with normal immune function can be used as a replacement of immunosuppressive mice to establish a T. asiatica oncosphere infection model. In addition, the T. asiatica eggs pre-incubated with sodium hypochlorite solution for 4 min have the strongest infection ability.
Assuntos
Taenia , Teníase , Animais , Tolerância Imunológica , Camundongos , Sus scrofa , SuínosRESUMO
Gelao ethnic group, an aboriginal population residing in southwest China, has undergone a long and complex evolutionary process. To investigate the genetic structure of this ancient ethnic group, mitochondrial DNA (mtDNA) polymorphisms of 102 Gelao individuals were collected and analyzed in this study. With the aid of the information extracted from control-region hypervariable segments (HVSs) I and II as well as some necessary coding-region segments, phylogenetic status of all mtDNAs under study were determined by means of classifying into various defined haplogroups. The southern-prevalent haplogroups B, R9, and M7 account for 45.1% of the gene pool, whereas northern-prevalent haplogroups A, D, G, N9, and M8 consist of 39.2%. Haplogroup distribution indicates that the Gelao bears signatures of southern populations and possesses some regional characters. In the PC map, Gelao clusters together with populations with Bai-Yue tribe origin as well as the local Han and the Miao. The results demonstrate the complexity of Gelao population and the data can well supplement the China mtDNA database.
Assuntos
Povo Asiático/genética , DNA Mitocondrial/genética , Etnicidade/genética , Polimorfismo Genético , China , Regiões Determinantes de Complementaridade/genética , Impressões Digitais de DNA , Bases de Dados Factuais , Pool Gênico , Variação Genética , Genética Populacional , Geografia , Haplótipos , Humanos , Mitocôndrias/genética , Fases de Leitura Aberta , Filogenia , Padrões de ReferênciaRESUMO
The gene frequency of 5 characters was reported in Buyi, Miao, Shui, Maonan, Dong, and Han nationalities in southern Guizhou, and analyzed for statistical significance among these nationalities. The results showed that: (1) For Hair forms and Nasal profile, the frequency of the dominant gene was lower than that of theirs recessive gene, but the opposite was true for Nostril forms. (2) Among different nationalities, the difference of gene frequency of Mongoloid fold, Nasal profile, Hair forms and Eye fold of the upper eyelid was quite significant, which was followed by that of Nostril forms.
