Evaluation of the coordinated development between Chinese cultural industry and scientific & technological innovation.

Based on the coupling and interaction relationship between China's cultural industry (CI) and scientific & technological innovation (STI), this study constructed an index system for their coordinated development. The weight of each indicator was determined by using the entropy value method (EVM), and the coupling coordination degree (CCD) model was used to calculate CCD and coordination degree of China's CI and STI from 2012 to 2020. On this basis, the key factors in the coupling effect were analyzed using grey correlation degree (GCD). The results demonstrate that: (1) there is a high-level coupling relationship between China's CI and technological innovation; (2) the level of coupling coordination between the two is generally on the rise, experiencing a development process from serious maladjustment to high coordination; (3) Industry resources, policy support and the cost of cultural undertakings are the endogenous factors restricting the development of CI, and the environment and output of STI are the key factors restricting the coupling and coordinated development of Chinese CI and STI.

Early-life phenanthrene exposure inhibits reproductive ability in adult zebrafish and the mechanism of action.

Phenanthrene (Phe) is a representative polycyclic aromatic hydrocarbon, and its ubiquity makes the risk assessment of Phe in aquatic ecosystems important. To assess the long-term effects of early-life Phe exposure on fish, the embryos of the model organism, zrbrafish (Danio rerio) were exposed to Phe at 0.05, 0.5, 5 and 50 nmol/L for 96 h and then raised to adulthood in clean water. Gonad development and reproductive functions were investigated in 120 day-old fish. The results showed that the percentage of spermatozoa in males and mature oocytes in females were decreased. The spawned egg numbers and the fertilization rate were reduced when the treated fish were mated with untreated fish. The transcription of genes involved in the brain-pituitary-gonadal axis was downregulated. The levels of both 17ß-estradiol and testosterone were significantly decreased in the 5 and 50 nmol/L groups compared with the control group. The methylation levels in the promotor of gnrh3 (encoding gonadotropin releasing hormone) were significantly elevated in the adult fish in the 5 and 50 nmol/L treatments, which might be associated with the downregulation of gnrh3 transcription. These results suggested that embryonic exposure to Phe can inhibit the reproductive ability of adult fish, which should be adequately emphasized in its risk assessment.

Embryonic exposure to benzo(a)pyrene inhibits reproductive capability in adult female zebrafish and correlation with DNA methylation.

This study was conducted to investigate the effects of embryonic short-term exposure to benzo(a)pyrene (BaP), a model polycyclic aromatic hydrocarbon, on ovarian development and reproductive capability in adult female zebrafish. In 1-year-old fish after embryonic exposure to BaP for 96 h, the gonadosomatic indices and the percentage of mature oocytes were significantly decreased in the 0.5, 5 and 50 nmol/L treatments. The spawned egg number, the fertilization rate and the hatching success were significantly reduced, while the malformation rate of the F1 unexposed larvae were increased. The mRNA levels of follicle-stimulating hormone, luteinizing hormone, ovarian cytochrome P450 aromatase cyp19a1a and cyp19b, estrogen receptor esr1 and esr2, and hepatic vitellogenin vtg1 and vtg2 genes, were down-regulated in adult female zebrafish that were exposed to BaP during embryonic stage. Both 17ß-estradiol and testosterone levels were reduced in the ovary of adult females. The methylation levels of the gonadotropin releasing hormone (GnRH) gene gnrh3 were significantly increased in the adult zebrafish brain, and those of the GnRH receptor gene gnrhr3 were elevated both in the larvae exposed to BaP and in the adult brain, which might cause the down-regulation of the mRNA levels of gnrh3 and gnrhr3. This epigenetic change caused by embryonic exposure to BaP might be a reason for physiological changes along the brain-pituitary-gonad axis. These results suggest that short-term exposure in early life should be included and evaluated in any risk assessment of pollutant exposure to the reproductive health of fish.