RESUMO
Many studies reported that long noncoding RNAs (lncRNAs) play a critical role in gastric cancer (GC) metastasis and tumorigenesis. However, the underlying mechanisms of lncRNAs in GC remain unexplored to a great extent. LINC01537 expression level was detected using quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC). Its biological roles in GC were then investigated using functional experiments. In order to investigate the underlying mechanism of LINC01537 in GC, RNA pull-down, RNA immunoprecipitation, and ubiquitination assays were performed. LINC01537 was significantly overexpressed in GC tissues and associated with a poor prognosis. Functional experimental results revealed that LINC01537 promoted the proliferation, invasion, and migration of GC cells. The animal experiments revealed that LINC01537 promoted tumorigenesis and metastasis in vivo. Mechanistically, LINC01537 stabilizes RIPK4 by reducing the binding of RIPK4 to TRIM25 and reducing its ubiquitination degradation, thereby promoting the expression of the NF-κB signaling pathway. According to our findings, the LINC01537-RIPK4-NF-κB axis promoted GC metastasis and tumorigenesis.
RESUMO
It is showed that the empirical scatter-radius function (Day function) used to generate the equivalent-field tables is a good approximation to the results of normalized scatter-air ratios extracted from the British Journal of Radiology (BJR) beam data for depths up to 10 cm. Accuracy can be improved by making the Day function depend on depth. Equivalent squares, determined by sector integration of the original or modified Day functions, are suitable for megavoltage photon-beam dose calculations in irregular as well as rectangular fields. We make further simplification for the functions to calculate equivalent squares by no use of scatter dose data that is often difficult to get for hospitals. The simplified method has the same accuracy compared to the original or modified Day functions.
