Surveillance of Oncomelania hupensis distribution and water levels in Gaoyou sections of the eastern route project of the South-to-North Water Diversion Project following operation of the project / 中国血吸虫病防治杂志

Objective To understand the distribution of Oncomelania hupensis snails and changes of water levels in Gaoyou sections of the Li Canal following the operation of the eastern route project of the South-to-North Water Diversion Project. Methods The Oncomelania snails were monitored in the river banks and water bodies of Gaoyou sections of the Li Canal by means of systematic sampling combined with environmental sampling as well as collection of the floaters from 2014 to 2019, and the water levels were collected in Gaoyou sections of the Li Canal at the typical hydrological year before the operation of the eastern route project of the South-to-North Water Diversion Project and during the period between 2016 and 2019. Results A total area of 235.42 hm2 were investigated and a total of 75.8 kg floaters were collected in Gaoyou sections of the Li Canal from 2014 to 2019; however, no snails were found. The water level in Gaoyou sections of the Li Canal was predominantly high in the flood season and low in the dry season before the operation of the eastern route project of the South-to-North Water Diversion Project, and the water level was elevated in the dry season and relatively low in the flood season after the operation of the project. Conclusion Following the operation of the eastern route project of the South-to-North Water Diversion Project, the original river bank that is characterized by “land in winter and water in summer” has changed in Gaoyou sections of the Li Canal, which is not favorable for snail breeding.

[Analysis of endemic status of schistosomiasis in Gaoyou City from 1970 to 2009].

OBJECTIVE: To explore the changing rule of schistosomiasis endemic situation in Gaoyou City, so as to provide the evidences for schistosomiasis control in lake and marshland regions. METHODS: The endemic data of schistosomiasis of Gaoyou City from 1970 to 2009 were collected to analyze the endemic patterns of schistosomiasis in different control stages comprehensively. RESULTS: In the first years of infection control stage (1970-1975), transmission control stage (1976-1994) and transmission interruption stage (1995-2009), the prevalence rates of schistosomiasis were 4.20%, 0.80% and 0 in human and 3.00%, 0.51% and 0 in cattle respectively. In 1984, the positive rate of crowd stool tests fell to 0.04%, which was reduced by 99.05% compared with that in 1970. From 1970 to 1980, the positive rates of stool tests of people and livestock were positively correlated significantly (r = 0.67, P < 0.05). After 1985, no local infection patients or cattle were found. No schistosome infected Oncomelania hupensis snails were found from 1970 to 2009. CONCLUSIONS: The comprehensive control measures have been insisted in Gaoyou City in different prevention and control stages. Although the snail situation is undulate, the prevalence of schistosomiasis steadily declines and the interruption of transmission has achieved.

Effects of morphine on the development of chick embryos / 中国应用生理学杂志

<p><b>AIM</b>To investigate the effect of morphine on fetal movement, heart rate, hatch weight, hatch days and hatch rate.</p><p><b>METHODS</b>Morphine was injected into airspace of eggs and fetal movement, heart rate, hatch weight, hatch days and hatch rates were recorded.</p><p><b>RESULTS</b>Hatch days were shorter, hatch rates were lower and some chicks became motor disorder for morphine. Chicks with morphine exposure 20 mg/kg from E 12 to E 16 had highest hatch rate and lowest disable rate. Morphine reduced fetal movement, increased heart rate (P < 0.05).</p><p><b>CONCLUSION</b>The development of chick embryo is impaired by morphine exposure and the magnitude of these effects depends on the drug dose and the length of time that the developing organism is exposed to morphine.</p>

The expression of VP19 gene from prawn white spot syndrome virus in silkworm, Bombyx mori using host range-expanded HyNPV / 生物工程学报

Prawn white spot syndrome is caused by the pathogen prawn white spot syndrome virus (WSSV). VP19 is a vesicle membrane protein of WSSV. HyNPV (Hybrid of AcNPV and BmNPV) constructed by the recombination of BmNPV and AcNPV is a new hybrid virus having both of their advantages. The recombinant transfer vector pBlueBicHisC-vp19 and recombinant baculovirus HyNPV-VP19 were constructed on the basis of the successful cloning of VP19. Newly-molted silkworms Bombyx mori of fifth instar were inoculated by the recombinant virus. SDS-PAGE and Western blotting analysis showed a specific band, about 21kD, which was consistent with the expectation suggesting that the WSSV-VP19 gene was successfully expressed in silkworm bodies.