Tregs depletion aggravates activation of astrocytes by modulating IL-10/GXP4 following cerebral infarction.

Background: Tregs plays a critical role in the development of secondary injuries in diseases. Accumulating evidence suggests an association between ischemic stroke and renal dysfunction; however, the underlying mechanisms remain unclear. This study aimed to investigate the potential of Tregs in inhibiting the activation of astrocytes after focal cerebral infarction. Methods: This study aimed to investigate the renal consequences of focal cerebral ischemia by subjecting a mouse model to transient middle cerebral artery occlusion (tMCAO). Subsequently, we assessed renal fibrosis, renal ferroptosis, Treg infiltration, astrocyte activation, as well as the expression levels of active GPX4, FSP1, IL-10, IL-6, and IL-2 after a 2-week period. Results: In the tMCAO mouse model, depletion of tregs protected against activation of astrocyte and significantly decreased FSP1, IL-6, IL-2, and NLRP3 expression levels, while partially reversing the changes in Tregs. Mechanistically, tregs depletion attenuates renal fibrosis by modulating IL-10/GPX4 following cerebral infarction. Conclusion: Tregs depletion attenuates renal fibrosis by modulating IL-10/GPX4 following cerebral infarction.

[Antibiotic-resistant bacteria contamination in the air of different departments in hospital].

OBJECTIVE: To investigate the contamination of antibiotic-resistant bacteria in air of different departments in hospital. METHODS: From 2018.07 to 2021.06, 191 samples of the air-conditioning filter dust in three hospitals were collected. Antibiotic-resistant bacteria were isolated from the accumulated dust. The drug sensitivity test was conducted for Staphylococcus aureus, Acinetobacter baumannii and Enterobacteriaceae. RESULTS: A total of 119 samples were detected antibiotic-resistant bacteria from 191 samples, and the detection rate was 62.30%. The detection rate of different departments from high to low was surgical ward(68.29%) >intensive care unit(ICU)(59.62%) >medical ward(57.92%). A total of 362 strains of antimicribial-resistant organisms were isolated, mainly were Acinetobacter(28.73%), Pseudomonas(22.10%), Bacillus(22.10%), Staphylococcus(9.12%), etc. Among them, 72 strains of target organisms were detected, and the detection rate was 19.89%(72/362), the detection rate of different target bacteria from high to low was Acinetobacter baumannii(12.71%)>Enterobacteriaceae(4.72%)>Staphylococcus aureus(2.76%)(P<0.05). The drug sensitivity test showed that 41 strains of antimicribial-resistant organisms were detected, and the detection rate was 56.94%(41/72), including carbapenem-resistant Acinetobacter baumannii(CR-ABA), methicillin-resistant Staphylococcus aureus(MRSA), carbapenem-resistant Enterobacteriaceae(CRE), etc.24 strains of multidrug-resistant organisms(MDROs) were detected and the detection rate was 58.54%(24/41). The detection rate of different departments from high to low was ICU(80.00%)>medical ward(60.00%)>surgical ward(46.15%). CONCLUSION: There was contaminated by Acinetobacter baumannii, Staphylococcus aureus, Enterobacteriaceae in the air of hospitals, some of them were MDROs, mainly were detected in neurological ward, respiratory medical ward, hyroid and breast surgery ward, neurosurgery ward, cardiothoracic surgery ward, gallideulous surgical ICU and general ICU.

Highly Sensitive and Specific Detection of Mobilized Colistin Resistance Gene mcr-1 by CRISPR-Based Platform.

Mobilized colistin resistance (mcr-1) gene mediated by plasmid can cause the speediness dissemination of colistin-resistant strains, which have given rise to a great threat to the treatment of human infection. Hence, a rapid and accurate diagnosis technology for detecting mcr-1 is essential for the control of resistance gene. Here, a recombinase polymerase amplification (RPA) coupled with CRISPR/Cas12a platform was established for rapid, sensitive, and specific detection of mcr-1 gene. The analytical sensitivity of our assay is 420 fg per reaction in pure mcr-1-positive isolates, and the threshold of this method in spiked clinical samples was down to 1.6 × 103 ~ 6.2 × 103 CFU/mL (1.6 ~ 6.2 CFU/reaction). Moreover, the RPA-CRISPR/Cas12a system perspicuously demonstrated no cross-reactivity with other resistant genes. The entire experimental process included rapid DNA extraction (15 min), RPA reaction (30 min), CRISPR/Cas12a cleavage (5 min), and fluorescence testing (<10 min), which could be completed within 60 min. In summary, the RPA-CRISPR/Cas12a assay designed here provides a rapid diagnostic way for monitoring mcr-1 in clinic and livestock farm. IMPORTANCE This study promises a rapid and accurate assay (RPA-CRISPR/Cas12a) for the surveillance of mcr-1 gene, which causes the efficacy loss of colistin in clinical treatments. In addition, the established method is fit for "on-site" surveillance especially.