Impact of emerging pollutant florfenicol on enhanced biological phosphorus removal process: Focus on reactor performance and related mechanisms.

Florfenicol (FF), an emerging pollutant antibiotic that is difficult to biodegrade, inevitably enters sewage treatment facilities with high level. To date, however, the performance and related mechanism of FF on enhanced biological phosphorus removal (EBPR) have not been reported. In order to fill this gap, this work investigated the potential impacts of FF on EBPR and revealed the relevant mechanisms. The effect of FF on EBPR was dose-dependent, that was, low dose had no effect on EBPR, while high FF concentration inhibited EBPR. Mechanism investigation showed that FF had no effect on anaerobic phosphate release, but reduced oxic phosphorus uptake. Three-dimensional Excitation-emission Matrix fluorescence spectroscopy and X-ray photoelectron spectroscopy analysis showed that FF affected the structure and components of activated sludge extracellular polymers (EPS). High content of FF stimulated sludge to secrete more EPS. High level of FF reduced the relative abundance of microorganisms responsible for biological phosphorus removal. Microbiological community structure analysis indicated 2.0 mg FF/L increased the relative abundance of Candidatus_Competibacter and Terrimonas from 9.22 % and 12.49 % to 19.00 % and 16.28 %, respectively, but significantly reduced the relative abundance of Chinophagaceae from 11.32 % to 0.38 %, compared with the blank.

Migration, Transformation and Removal of Macrolide Antibiotics in The Environment: A Review.

Macrolide antibiotics (MAs), as a typical emerging pollutant, are widely detected in environmental media. When entering the environment, MAs can interfere with the growth, development and reproduction of organisms, which has attracted extensive attention. However, there are few reviews on the occurrence characteristics, migration and transformation law, ecotoxicity and related removal technologies of MAs in the environment. In this work, combined with the existing relevant research, the migration and transformation law and ecotoxicity characteristics of MAs in the environment are summarized, and the removal mechanism of MAs is clarified. Currently, most studies on MAs are based on laboratory simulation experiments, and there are few studies on the migration and transformation mechanism between multiphase states. In addition, the cost of MAs removal technology is not satisfactory. Therefore, the following suggestions are put forward for the future research direction. The migration and transformation process of MAs between multiphase states (such as soil-water-sediment) should be focused on. Apart from exploring the new treatment technology of MAs, the upgrading and coupling of existing MAs removal technologies to meet emission standards and reduce costs should also be concerned. This review provides some theoretical basis and data support for understanding the occurrence characteristics, ecotoxicity and removal mechanism of MAs.

Evaluating the effect of fluoxetine on mesophilic anaerobic dark biohydrogen fermentation of excess sludge.

Recently, the influence behavior of new pollutants in the environment has been widely concerned. However, the effect of antidepressants widely detected in excess sludge (ES) on biohydrogen production from anaerobic dark fermentation has never been explored. To fill this gap, fluoxetine (FLX), a typical antidepressant, was selected to evaluate its effect on ES mesophilic anaerobic dark biohydrogen fermentation. The results showed that FLX reduced biohydrogen production even at low content (0.1 mg/Kg). The biohydrogen yield was only 12.8 mL/g in the 1.8 mg/Kg (based on total suspended solids) FLX group, decreased by about 34.7%, compared with the control group (without FLX). Further mechanism investigation implied that high levels (more than 0.6 mg/Kg) of FLX reduced every step associated with the biohydrogen production. FLX reduced the concentration of ammonia nitrogen and phosphate in fermentation broth. FLX also had a significant negative effect on enzyme activity in ES dark fermentation.

Effect of fluoxetine on enhanced biological phosphorus removal using a sequencing batch reactor.

In this work, the potential impact of emerging pollutant Fluoxetine (FLX) on enhanced biological phosphorus removal (EBPR) was systematically investigated using the sequencing batch reactor. The experimental results showed that even 200 µg/L FLX had no significant effect on EBPR during the short-term exposure. However, in the long-term exposure test, high dosage of FLX inhibited EBPR. 200 µg/L FLX induced biological phosphorus removal efficiency dropped to 71.3 ± 2.1%, significantly lower than that of the blank. The mechanism investigation showed that high concentration of FLX reduced anaerobic phosphorus release and oxic phosphorus absorption, and the consumption of organic matter during the anaerobic period. In addition, FLX decreased the synthesis of intracellular polymer polyhydroxyalkanoates (PHA), but promoted the metabolism of glycogen and polyhydroxyvalerate. FLX reduced the activity of key enzymes in EBPR and the relative abundance of Accumulibacter, but improved the relative abundance of Candidatus Competibacter.