RESUMO
The gene encoding the neutral phytase nphy was cloned from Bacillus subtilis by polymerase chain reaction (PCR). Nucleotide sequence analysis of nphy revealed the presence of an open reading frame of 1152 bp coding for 383 aa. The start codon was followed by a sequence coding for a putative signal peptide of 26 aa in length. The nphy without original signal peptide encoding sequence was cloned into E. coli expression plasmid pTYB40. The result of SDS-PAGE of the phytase expressed in E. coli showed that the nphy had been overexpressed. The expressed phytase was over 40% of the total soluble protein of E. coli, and has normal bioactivity.
Assuntos
6-Fitase/genética , Bacillus subtilis/genética , Escherichia coli/genética , Proteínas Recombinantes/biossíntese , 6-Fitase/biossíntese , 6-Fitase/química , Sequência de Aminoácidos , Sequência de Bases , Clonagem Molecular , Dados de Sequência MolecularRESUMO
OBJECTIVE: To investigate the effect of the emergent repair of peripheral nerve injury of the wrist. METHODS: From July 1993 to December 1997, 17 cases were admitted, which 21 injured peripheral nerves were repaired emergently. Among them, there were 11 cases of median nerve injury, 2 cases of ulnar nerve injury and 4 cases of median and ulnar nerve injury. All the nerves were ruptured completely except one which was partially ruptured. The emergent operation was taken and the injured nerves were repaired by microsurgical technique. RESULTS: Followed up 6 to 18 months after operation, 95.25% injured nerves had good outcome. CONCLUSION: Because of the specific structure of the wrist, nerve injury at this part need to be repaired emergently. It can enhance the regeneration of the injured nerve, preserve the function of the intrinsic muscle of hand, and decrease the local adhesion.
Assuntos
Traumatismos dos Nervos Periféricos , Nervos Periféricos/cirurgia , Traumatismos do Punho/cirurgia , Adolescente , Adulto , Feminino , Seguimentos , Humanos , Masculino , Nervo Mediano/lesões , Nervo Mediano/cirurgia , Microcirurgia , Pessoa de Meia-Idade , Nervo Ulnar/lesões , Nervo Ulnar/cirurgiaRESUMO
The effect of exogenous nerve growth factor (NGF) examined on the neural repair of adult rabbit sciatic nerve was evaluated in the present study. A nerve regeneration chamber was created by suturing the proximal and distal ends of a transected sciatic nerve into a silicone chamber. A gap of 6 mm in chamber was left after removal of a 3 mm piece of nerve in the distal ends and insertion of the proximal and distal stumps into the chamber. Animals were operated on bilaterally, one side of the chamber was filled with a 1 mg/ml NGF/normal saline (NS) (experimental) and the contralateral side with NS (control). The regenerated nerves from within the silicone chamber were dissected and fixed 1 to 5 weeks following surgery for histological studies at both the light microscopic and ultrastructural levels. The NGF chamber showed a more mature regenerated nerve based on a larger diameter of the regenerated nerve trunk, a great number of axons, and thicker myelin sheaths.
