Analysing the mechanism of food waste anaerobic digestion enhanced by iron oxide in a continuous two-stage process.

The food waste (FW) digestion performance can be enhanced by introducing iron oxide (IO) into digesters. However, the role of IO in continuous two-stage digesters in enhancing the FW anaerobic digestion remains unclear. In this study, the effect of IO on the bioenergy recovery from a two-stage digestion process was investigated. The bioenergy recovery was significantly increased by up to 208.43 % with IO addition. The activities of dehydrogenase, α-amylase, and protease increase by 0.82-1.44, 7.24-14.56 and 7.97-20.45 times, respectively, as compared with that of the blank. With IO addition, the metabolic pathway in hydrolytic-acidogenic (HA) reactor shifted from lactic acid fermentation to butyric fermentation, which promoted stable methane production in methanogenic (MG) reactor. The activity of coenzyme F420 increased by 19.19-39.01 times, indicating that IO facilitated FW digestion by promoting hydrogenotrophic methanogenesis. The enhancement in the enzyme activity was attributable to the Fe2+ generated by dissimilatory iron reduction. According to the microbial analysis, IO enhanced interspecies hydrogen transfer between Methanobacterium and Syntrophomonas. Furthermore, IO improved direct interspecies electron transfer between Geobacter sulfurreducens and Methanosarcina. The effluent recirculation strategy greatly facilitated the hydrolysis and acidification of FW, which was critical for improving the two-stage process performance.

Enhancing methane production from food waste with iron-carbon micro-electrolysis in a two-stage process.

A two-stage process, consisting of a leach-bed reactor (LBR) and an up-flow anaerobic sludge blanket reactor (UASB), has been commonly adopted to improve food waste anaerobic digestion. However, its application is limited due to low hydrolysis and methanogenesis efficiencies. This study proposed a strategy of incorporating iron-carbon micro-electrolysis (ICME) into the UASB and recirculating its effluent to the LBR to improve the two-stage process efficiency. Results showed that the integration of the ICME with the UASB significantly increased the CH4 yield by 168.29%. The improvement of the food waste hydrolysis in the LBR mainly contributed to the enhanced CH4 yield (approximately 94.5%). The enrichment of hydrolytic-acidogenic bacterial activity, facilitated by the Fe2+ generated through ICME, might be the primary cause of the improved food waste hydrolysis. Moreover, ICME enriched the growth of hydrogenotrophic methanogens and stimulated the hydrogenotrophic methanogenesis pathway in the UASB, contributing partially to the enhanced CH4 yield.

Recovering and potentially applying of alginate like extracellular polymers from anaerobic digested sludge.

Extracellular polymeric substances (EPS) are biopolymers contained in both aerobic and anaerobic sludge. In EPS, alginate like extracellular polymers (ALE) is thought as a highly valued material, which have been widely studied with aerobic sludge. Nevertheless, a curiosity on ALE remains in anaerobic digested sludge (ADS). With 5 different sludge sources, anaerobic digestion of excess sludge was conducted in a batch mode, and then ADS was used to extract ALE and to analyze its physicochemical properties for potential applications. The yield of ALE extracted from ADS (ALE-ADS) ranged from 119.4 to 179.4 mg/g VSS. The compositional characteristics of ALE-ADS observed by FT-IR, 3D-EEM and UV-Vis spectroscopy revealed that there were minor differences in the composition and property of ALE-ADS but a similarity of 62 %-70 % to a commercial alginate remained in terms of chemical functional groups. Moreover, ALE-ADS composed of 1,4-linked ß-d-mannuronic acid (M) and 1,4 α-l-guluronic acid (G) residues that form blocks of GG (20.8 %-33.8 %), MG (12.8 %-30.1 %) and MM (6.6 %-15.1 %), respectively. Based on the gel-forming capacity, film-forming property, adsorbility, and amphiphilicity, ALE-ADS seems potential as a water-proof coating with even a better performance than the commercial alginate, as a seed coating with an increased germination rate, and as a bio-adsorbent with a similar performance to the commercial alginate and ALE from aerobic sludge.

Unravelling key factors controlling vivianite formation during anaerobic digestion of waste activated sludge.

As a product of phosphorous recovery from anaerobic digestion (AD) of waste activated sludge (WAS), vivianite has received increasing attention. However, key factors controlling vivianite formation have not yet been fully addressed. Thus, this study was initiated to ascertain key factors controlling vivianite formation. A simulation of chemical equilibriums indicates that interfering ions such as metallic ions and inorganic compounds may affect vivianite formation, especially at a PO43-concentration lower than 3 mM. The experiments demonstrated that the rate of ferric bio-reduction conducted by dissimilatory metal-reducing bacteria (DMRB) and the competition of methane-producing bacteria (MPB) with DMRB for VFAs (acetate) were not the key factors controlling vivianite formation, and that ferric bio-reduction of DMRB can proceed when a sufficient amount of Fe3+ exists in WAS. The determined affinity constants (Ks) of both DMRB and MPB on acetate revealed that the KHAc constant (4.2 mmol/g VSS) of DMRB was almost 4 times lower than that of MPB (15.67 mmol/g VSS) and thus MPB could not seriously compete for VFAs (acetate) with DMRB. As a result, vivianite formation was controlled mainly by the amount of Fe3+ in WAS. In practice, a Fe/P molar ratio of 2:1 should be enough for vivianite formation in AD of WAS. Otherwise, exogenously dosing Fe3+ or Fe2+ into AD must be applied in AD.

Exploring the roles of zero-valent iron in two-stage food waste anaerobic digestion.

This research investigated the roles of zero-valent iron (ZVI) in a two-stage food waste digestion process. ZVI was added separately to hydrolytic-acidogenic (HA) and methanogenic (MG) stages to understand its impacts on FW hydrolysis-acidification, methanogenesis and bioenergy recovery efficiency. Results showed that ZVI effectively enhanced the overall performance of digestion as compared with the controls without ZVI. Supplementing with ZVI could facilitate the HA process along with faster hydrogen generation. In addition, ZVI shortened the lag phase of MG stage by 42.43-57.23% and raised the maximum methane production rate and yield by 33.99-38.20% and 11-13%, respectively, compared with the controls. Supplementing ZVI to the HA stage could simultaneously raise the bioenergy recovery efficiency of the HA and MG stages by 71.92% and 96.96%, respectively. Further studies demonstrated that iron corrosion contributed little to hydrogen and methane production. The enrichment of syntrophic bacteria, Pseudomonas, and methanogens, and the enhancement of electron transfer among those microbes was supposed to be the main possible mechanism for the enhancement of methanogenesis with ZVI assisted.

Extracellular enzyme and microbial activity in MSW landfills with different gas collection and leachate management practices.

The performance of simulated municipal solid waste (MSW) landfills with two different biogas collection practices - (1) upward and upward-downward biogas flow collection (LT-TB) in sequence and (2) simultaneous upward-downward biogas flow collection (LTB) from the beginning of the anaerobic degradation process - was investigated in terms of landfill gas and leachate, enzyme activity, and microbial community structure associated with MSW compression and leachate recirculation. The cumulative methane volume in LTB was 1.5 times higher than that in LT-TB. With MSW compression and leachate recirculation, amylase and lipase activity were enhanced in LTB. In LT-TB, the activities gradually decreased after reaching a peak with compression. The two biogas collection strategies influenced the community structure and activity of bacteria and archaea. The upward and downward gas collection flow with waste compression and leachate recirculation improved the environment for enriching bacterial phyla Firmicutes, Proteobacteria, and Synergistetes and genus Methanosarcina in Archaea.

Iron oxide alleviates acids stress by facilitating syntrophic metabolism between Syntrophomonas and methanogens.

Anaerobic digestion (AD) is a promising technology for food waste management, but frequently restricted with long lag phase as a consequent of acidification. Two laboratory experiments were conducted to investigate the effects of iron materials on food waste AD. Experiment 1 compared the effects of iron oxide (IO) and zero valent iron (ZVI) on AD performance. The results showed that both IO and ZVI could enhance methane (CH4) generation, but IO showed better performance regarding the reduction of lag phase. The lag phase of the reactor supplemented with IO was 17.4% and 42.7% shorter than that of the reactor supplemented with ZVI and the control, respectively. Based on these results, experiment 2 was designed to examine the role of IO in alleviation of acid stress at high substrate to inoculum (SI) ratio. The results showed that supplemented IO into reactor could ensure a successful methanogenesis when operating at high SI ratio, while IO-free reactor was failed to generate CH4 although operating for 77 days. Supplementing IO into the reactor after 48 h of digestion could restore the CH4 generation, though its lag phase was 2.6 times of the reactor supplemented with IO at the beginning of the digestion. Microbial community structure analysis revealed that IO could simultaneously enrich Syntrophomonas and methanogens (i.e. Methanobacterium, Methanofollis and Methanosarcina), and might promote electron transfer between those two types of microbes, which were critical for achieving an effective methanogenesis.

Geotextile clogging at different stages of municipal solid waste landfills co-disposed with bottom ash.

Co-disposal of bottom ash (BA) with municipal solid waste (MSW) in landfills is a common way for BA management. However, BA co-disposal in MSW landfills may accelerate geotextile clogging and reduce the performance of leachate collection system. This study compared geotextile clogging in a simulated MSW landfill leachate (MSWL) and a BA co-disposed landfill leachate (BAL) at different landfill stages. Geotextile clogging test was conducted using the MSWL and BAL taken from the simulated landfills on the 10th, 80th, 140th and 200th day, respectively. The results demonstrated that geotextile clogging varied with landfill age, due to the change of leachate characteristics. The mass of clogging material in geotextiles with BAL increased from 0.45 g to 2.74 g, which was 43.87%-63.73% greater than those with MSWL. The formation of biofilm was the main contributor for the geotextile clogging. At the same stage, the amount of biofilm formed on geotextile in different leachate was comparable. However, the amounts of CaCO3 precipitation on geotextile in BAL were 3.85-10.44 times of those in MSW leachate. The pH of leachate played a critical role in CaCO3 precipitation. The microbial analysis revealed that the co-disposal of the BA greatly influenced the microbial community diversity and structure.

Enhancement of hydrogen production using untreated inoculum in two-stage food waste digestion.

This research investigated the possibility to enhance H2 production using untreated inoculum in a two-stage hydrogen-methane process from food waste. Batch experiments were conducted to evaluate the H2 production efficiency at different F/M ratios (ranging from 1:1 to 64:1). The results showed that when a proper F/M ratio was selected, significant H2 production was feasible to be achieved even inoculated with untreated anaerobic sludge. Among the F/M ratios studied, maximum H2 yield (217.98 mL H2 g VS-1 FW) was found in the digester at the F/M of 64:1, which was 93.75 times higher than that of the digester at the F/M of 1:1. Higher hydrogen yield was achieved at the greater F/M ratio, due to the enrichment of the H2 producing bacteria and the reduction of the antagonistic bacteria. The two-stage process allowed more stable methane production and higher overall energy yield compared to the single-stage process.

Effect of nickel-containing activated carbon on food waste anaerobic digestion.

Anaerobic digestion (AD) is frequently restricted with the long lag phase and low methane (CH4) production rate. Laboratory batch experiments were conducted to investigate the impact of different supplements on the performance of food waste AD, including AC-Ni, AC, and Ni. Results showed that the lag phase of AD was reduced with the addition of those supplementations. Compared with the control group without any supplementation, the AC-Ni could shorten the lag phase by 67% and increase the maximum CH4 production rate by 50%, respectively. The speciation analysis indicated that the environmental risks of the AC-Ni was reduced by 30% after digestion. Microbial community structure analysis revealed that the AC-Ni promoted the evolution and activity of the hydrolytic-fermentative bacteria (e.g. Firmicutes and Bacteroidetes) and methanogens (e.g. Methanobacterium, Methanoregula and Methanomassiliicoccus). This study suggested that the AC-Ni waste could be feasible to be applied to enhance the performance of AD.