Magnetic Microbead-Based Herringbone Chip for Sensitive Detection of Human Immunodeficiency Virus.

The microfluidic chip-based nucleic acid detection method significantly improves the sensitivity since it precisely controls the microfluidic flow in microchannels. Nonetheless, significant challenges still exist in improving the detection efficiency to meet the demand for rapid detection of trace substances. This work provides a novel magnetic herringbone (M-HB) structure in a microfluidic chip, and its advantage in rapid and sensitive detection is verified by taking complementary DNA (cDNA) sequences of human immunodeficiency virus (HIV) detection as an example. The M-HB structure is designed based on controlling the magnetic field distribution in the micrometer scale and is formed by accumulation of magnetic microbeads (MMBs). Hence, M-HB is similar to a nanopore microstructure, which has a higher contact area and probe density. All of the above is conducive to improving sensitivity in microfluidic chips. The M-HB chip is stable and easy to form, which can linearly detect cDNA sequences of HIV quantitatively ranging from 1 to 20 nM with a detection limit of 0.073 nM. Compared to the traditional herringbone structure, this structure is easier to form and release by controlling the magnetic field, which is flexible and helps in further study. Results show that this chip can sensitively detect the cDNA sequences of HIV in blood samples, demonstrating that it is a powerful platform to rapidly and sensitively detect multiple nucleic acid-related viruses of infectious diseases.

Magnetically driven nanorobots based on peptides nanodots with tunable photoluminescence for rapid scavenging reactive α-dicarbonyl species and effective blocking of advanced glycation end products.

The present work constructed magnetically driven nanorobots by conjugating the photoluminescent ß-alanine-histidine (ß-AH) nanodots to superparamagnetic nanoparticles (SPNPs) for simultaneously sensitive determination and fast trapping RDS in food processing, achieving efficient regulation of advanced glycation end products (AGEs) risk. Bio-derivative ß-AH nanodots with orderly self-assembly nanostructure and tunable photoluminescent properties served as both biorecognition elements to effectively bind and scavenge the reactive α-dicarbonyl species (RDS), as well as the indicator with sensitive fluorescence response in the food matrix. The magnetically driven nanorobots with excellent biosafety of endogenous dipeptides displayed a high binding capacity of 80.12 mg g-1 with ultrafast equilibrium time. Furthermore, the magnetically driven nanorobots achieved rapid removal of the RDS with the manipulation of the external magnetic field, which enabled intercepting AGEs generation without byproducts residual as well as ease-of-operation. This work provided a promising strategy with biosafety and versatility for both accurate determination and efficient removal of hazards.

Magnetically Controlled Nanorobots Based on Red Emissive Peptide Dots and Artificial Antibodies for Specific Recognition and Smart Scavenging of Nε-(Carboxymethyl)lysine in Dairy Products.

Food-borne advanced glycation end products (AGEs) are highly related to various irreversible diseases, and Nε-(carboxymethyl)lysine (CML) is the typical hazardous AGE. The development of feasible strategies to monitor and reduce CML exposure has become desirable to address the problems. In this work, we proposed magnetically controlled nanorobots by integrating an optosensing platform with specific recognition and binding capability, realizing specific anchoring and accurate determination as well as efficient scavenging of CML in dairy products. The artificial antibodies offered CML imprinted cavities for highly selective absorption, and the optosensing strategy was designed based on electron transfer from red emissive self-assembling peptide dots (r-SAPDs) to CML, which was responsible for the identity, response, and loading process. The r-SAPDs overcame the interference from autofluorescence, and the limit of detection was 0.29 µg L-1, which bestowed accuracy and reliability for in situ monitoring. The selective binding process was accomplished within 20 min with an adsorption capacity of 23.2 mg g-1. Through an external magnetic field, CML-loaded nanorobots were oriented, moved, and separated from the matrix, which enabled their scavenging effects and reusability. The fast stimuli-responsive performance and recyclability of the nanorobots provided a versatility strategy for effective detection and control of hazards in food.

Facile construction of magnetic core-shell covalent organic frameworks as efficient solid-phase extraction adsorbents for highly sensitive determination of sulfonamide residues against complex food sample matrices.

Integration of advanced sample pretreatment techniques, with the involvement of functional nano/micro-materials as adsorbents, is of great importance and value for food-safety precise inspection. For now, the major demands for functional adsorbents are ease of fabrication, fast adsorption and separation performance, low toxicity, robustness, and reusability. In the present work, core-shell structured magnetic covalent organic frameworks (COFs) that employed Fe3O4 microspheres as the magnetic core and TpBD COFs as the adsorption shell have been successfully constructed as efficient solid phase extraction (SPE) adsorbents for complex food sample analysis. In favor of the combination of magnetic separation and effective preconcentration, the proposed magnetic COF-SPE method gave a rapid detection performance of the simultaneous detection of ten sulfonamide residues as well as high sensitivity, with detection limits in the range of 0.28-1.45 µg L-1 under the optimized experimental conditions. The Fe3O4@TpBD core-shell adsorbents also demonstrated good stability, robust SPE preconcentration ability, excellent determination recovery, and good reusability. The applicability of the developed SPE method was well demonstrated by real sample analysis, with the recoveries ranging from 82-94%. Through this example, it was believed that the new emerging porous nano/micro-materials, like COFs, metal-organic networks, or hybrid structures, would play more and more important roles as functional materials in food-safety inspection, especially for highly efficient determination of targets against complicated food sample matrices.

Spherical covalent organic frameworks as advanced adsorbents for preconcentration and separation of phenolic endocrine disruptors, followed by high performance liquid chromatography.

As a promising generation of porous micro-materials, covalent organic frameworks (COFs) have great potentials for applications in separation and adsorption. In the present study, an advanced food-safety inspection method involving COFs as the adsorbents of solid phase extraction (SPE) is proposed for sensitive and accurate determination of target hazardous substances. Typical spherical TpBD COFs with large surface area and superior chemical stability were utilized as adsorbents for the preconcentration of phenolic endocrine disruptors (PEDs), followed by high performance liquid chromatography (HPLC) analysis. The well-prepared TpBD COFs were encapsulated in SPE cartridges and applied in food research, namely, for the separation and enrichment of four target endocrine disruptors in food samples. The possible factors influencing the SPE performance including the composition of the sample solvent, sample solution pH, sample flow rate, composition of the eluent, and the volume of the eluent were investigated and optimized. Due to the porous architecture and superior surface area of spherical TpBD, the enrichment of analytes via a COF-filled SPE column gave extremely low detection limits of 0.056-0.123 µg L-1 along with a wide linear range of 0.5-100 µg L-1 for all the analytes. Nine parallel determinations of the mixed standard with a concentration of 10 µg L-1 produced the relative standard deviations of 2.23-3.08%, indicating the excellent repeatability of the COF-SPE assay. This study can open up a new route for the employment of COFs as efficient SPE adsorbents for the enrichment and quantification of trace/ultra-trace hazardous materials in complex food samples.

Fabrication of an activatable hybrid persistent luminescence nanoprobe for background-free bioimaging-guided investigation of food-borne aflatoxin in vivo.

The development of in situ and real-time analytical methods for specifically probing food-borne hazardous substances is promising for clarifying their harmful behaviors and related disease mechanisms inside the living body through in situ investigation of their in vivo behaviors. Herein, optical nanoimaging with the ability of in situ non-damage detection and real-time monitoring was introduced for specific recognition of aflatoxin in cellular levels and in vivo via the fluorescence resonance energy transfer (FRET) protocol. Persistent luminescence nanophosphors (PLNPs) with distinct advantages of improved sensitivity and signal-to-noise ratio were employed in in vivo bioimaging as photoluminescence nanoprobes, while copper sulfide nanoparticles were utilized as the quencher. Due to their long-lasting afterglow, PLNPs do not require external illumination before imaging, effectively eliminating the scattering light and autofluorescence from the biological matrix that can occur during in situ excitation. The proposed FRET imaging assay achieved high sensitivity and specificity as well as improved imaging resolution for the target aflatoxin present in vivo. This study will provide insights towards advanced methodology for the applications of bioimaging in food safety, and could potentially provide an advisory roadmap for bioimaging-guided exploration and mediation of food-borne hazards to human health.