The Emerging Role of Exosomes in Oral Squamous Cell Carcinoma.

Oral cancer constitutes approximately 2% of all cancers, while the most common type, oral squamous cell carcinoma (OSCC) represents 90% of oral cancers. Although the treatment of OSCC has improved recently, it still has a high rate of local recurrence and poor prognosis, with a 5-year survival rate of only 50%. Advanced stage OSCC tends to metastasize to lymph nodes. Thus, exploring new therapeutic strategies for OSCC is therefore an urgent priority. Exosomes, the small membrane vesicles derived from endosomes, have been detected in a wide array of bodily fluids. Exosomes contain a diversity of proteins, mRNAs, and non-coding RNAs, including microRNAs, long non-coding RNAs, piRNAs, circular RNAs, tsRNAs, and ribosomal RNAs, which are delivered to neighboring cells or even transported to distant sites. Exosomes have been associated with the tumorigenesis of OSCC, promote the proliferation, colonization, and metastasis of OSCC by transferring their contents to the target cells. Furthermore, exosomes are involved in the regulation of the tumor microenvironment to transform conditions favoring cancer progression in vivo. In this review, we summarize the crucial role of exosomes in the tumorigenesis and progression of OSCC and discuss the potential clinical application of exosomes in OSCC treatment.

[Three-dimensional analysis for changes in dentigerous cyst-associated maxillary canines after marsupialization in adolescents].

OBJECTIVE: To observe three-dimensional changes of dentigerous cyst-associated maxillary canines (DCAMC) in adolescents after marsupialization by using cone beam computed tomography (CBCT).
 Methods: A total of 34 DCAMC patients with dentigerous cyst aged 10-14 were divided into central type and lateral type, while canines on the non-cyst side served as a control. A three-dimensional reference frame was set up to analyze the position, angle changes and influential factors for DCAMC by using CBCT before operation and in 3-6 month after operation.
 Results: 1) Compared with pre-operation, there was significant vertical movement in DCAMC after marsupialization. Tip of tooth moved labially while tooth axis inclined mesiodistally; 2) Horizontal, vertical movement and mesiodistal inclination of DCAMC were significantly greater than those in the health side; 3) Compared with the lateral type DCAMC, the central type DCAMC showed a significant changes in labial inclination; 4) Horizontal movement, mesiodistal and labial inclination of lateral type DCAMC were all evidently greater than those of the central type DCAMC; 5) Vertical movement and inclination of DCAMC after marsupialization were significantly correlated to the time interval (r=0.354, 0.374, both P<0.05), while vertical movement of cuspid in health side was significantly negative correlated with the patients' age and the level of root formation (r=-0.506, -0.721, both P<0.01).
 Conclusion: DCAMC in adolescents can obtain obvious changes in position and angles after marsupialization regardless of the level of root formation, which is beneficial for further orthodontic treatment.

[Three dimensional measurement of craniofacial hard tissues for the Han nationality with normal occlusion in Changsha area].

OBJECTIVE: To explore the influence of gender and age on the measurement of cone beam CT (CBCT) in normal and occluded craniofacial hard tissues for Han nationality in Changsha area.
 Methods: A total of 80 CBCT data of normal and occluded craniofacial hard tissues for Han nationality in Changsha area were collected, including 40 adults [16 males and 24 females, aged (17.57±1.83) years] and 40 children[16 males and 24 females, age (11.50±1.26) years]. Three-dimensional cephalometric measurements were carried out by Dolphion Imaging software, and the influence of gender and age was compared.
 Results: The symmetry of craniofacial hard tissues in male and female in the adults was good (the maximum asymmetric rate R was 6.39% and the minimum asymmetric rate R was 0.08%). There was no significant differencein the anterior palatal width, palatal width, the upper incisor protrusion distance, the lower incisor protrusion distance, hyoid-mandibular plane, basal epiglottis width, and palatal plane airway width between the males and females (all P>0.05); while the remaining indicators were significantly higher in males than those in females (all P<0.05). There was no significant gender difference in angle indicators (all P>0.05). There were significant differences in the posterior height, the maxillary length, the lower incisor protrusion, and the mandibular plane angle between the adults and the children(all P<0.05); but the rest of indicators were not significant difference (all P>0.05).
 Conclusion: The symmetry of craniofacial hard tissues for Han nationality in Changsha area is good. The gender and age affect the line space measurements but not affect the most of the angle measurements.

[Three-dimensional morphological features of temporomandibular joint in skeletal malocclusion Class III patients with different vertical skeletal facial types].

OBJECTIVE: To investigate three-dimensional morphological features of temporomandibular joint in skeletal malocclusion Class III with different vertical skeletal facial types.
 Methods: A total of 46 adults with skeletal malocclusion Class III were enrolled and allocated into a normal-angle, a high-angle and a low-angle groups. All patients were undergone cone beam computed tomography (CBCT). Images of temporomandibular joint were reconstructed and analyzed by Invivo 5. Nine measurements relevant to the positions and forms of condyle as well as joint spaces were performed.
 Results: Significant differences were not observed between the 2 sides of each group among skeletal malocclusion Class III (P>0.05). Compared with the high-angle and normal-angle patients, the low-angle patients had larger superior spaces, bigger gradients of the articular tublecle, longer condyle, and larger internal spaces between the condyle and the fossa. Compared with the low-angle and normal-angle patients, the high-angle patients had smaller condylar antero-posterior diameters.
 Conclusion: Class III malocclusion patients with different vertical skeletal patterns have different positions and forms of condyles, which should be paid attention to in clinic.

[Esthetic evaluation of frontal smile on different crown heights of lateral incisor and canine].

OBJECTIVE: To investigate the effect of different crown heights of lateral incisor and canine on smile esthetics perception between orthodontists and patients.
 Methods: A total of 31 orthodontists and 56 patients on smile aesthetics perception were investigated. We adjusted the height of lateral incisor and canine to get 20 kinds of anterior tooth area morphology by Photoshop and asked interviewees to grade. Scores of satisfaction were recorded by Likert method.
 Results: Subjects in the 2 groups preferred smiles with upper anterior teeth edge parallel to lower lip. There was better acceptance for longer canines and less satisfaction at shorter lateral incisors in patients. Patients also got higher discrete degree of evaluation results and more rigorous about smile esthetics than orthodontists.
 Conclusion: Orthodontists and patients have different satisfaction at esthetics of anterior teeth. Esthetics preference should be considered in orthodontic treatment schedule.

A squamous odontogenic tumor following an orthodontic micro-screw: A rare case report and review of the literature.

We reported a very rare case of squamous odontogenic tumor(SOT) in a 23-year-old female. The tumor arose after an implanting operation of an orthodontic micro-screw, and was definitely diagnosed by the histopathological examination. Based on the case report and a review of the literature, we discussed about the general features, differential diagnosis and pathogenesis of SOT.

Balance Preferences with Performance in Group Role Assignment.

Role assignment is a critical element in the role-based collaboration process. There are many factors to consider when decision makers undertake this task. Such factors include a decision maker's preferences and the team's performance. This paper proposes a series of methods, relative to these factors, to solve the group role assignment with balance problem through an association with the one clause at a time approach that is a well-accepted and logic-based association rule mining method. The proposed methods are verified by simulation experiments. The experimental results present the practicability of the proposed solutions. Using the proposed methods, decision makers need only to establish coarse-grain preferences. The fine-grain preferences can be mined. Furthermore, a balance is obtained between the fine-grain preferences and the team's performance.