RESUMO
OBJECTIVE: To investigate the effects of sodium alginate gels on marrow mesenchymal stem cell transplantation for repair of spinal cord injury (SCI) in mice. METHODS: In the present study, effects of different sterilization methods and concentrations of sodium alginate gels were examined. Marrow mesenchymal stem cells (mMSCs) were isolated from mice and cultured. Cells were cultured with sodium alginate gels and MTT assay was applied to determine the cell viability. Mice spinal cord injury was induced by spinal cord transection. mMSCs were transplanted into the cavity of injured spinal cord with sodium alginate gels. The effects of sodium alginate gel were assessed by BMS scales and immunofluorescence staining for NF-200. RESULTS: Compared with liquid form, solid form sodium alginate gel prepared with high pressure vapor sterilization had a better effect on cell viability. SCI mice treated with 10 % sodium alginate gel and mMSCs achieved higher score in BMS scale as well as higher expression of NF-200 compared with the untreated SCI group. CONCLUSION: Sodium alginate gel prepared with solid form sterilization induces mMSCs proliferation and thus can be used as the carrier of stem cell in treatment of SCI.
Assuntos
Alginatos/administração & dosagem , Transplante de Células-Tronco Mesenquimais/métodos , Traumatismos da Medula Espinal/terapia , Alginatos/uso terapêutico , Animais , Modelos Animais de Doenças , Géis , Ácido Glucurônico/administração & dosagem , Ácido Glucurônico/uso terapêutico , Ácidos Hexurônicos/administração & dosagem , Ácidos Hexurônicos/uso terapêutico , Masculino , Camundongos , Camundongos Endogâmicos ICRRESUMO
The present study was designed to investigate the neuroprotective effects of Ca(2+)-dependent phospholipid-binding protein annexin II and a secreted protein Reg-2 (regeneration gene protein 2) in spinal cord injury (SCI) model produced by contusion SCI at T(9) using the weight drop method. The agents were delivered intrathecally with Alzet miniosmotic pumps. We found that annexin II and Reg-2 remarkably reduced neuronal death, attenuated tissue damage and alleviated detrimental inflammation in vivo; meanwhile, a significant increase in white matter sparing and myelination area was observed. The propriospinal axons and long-distance supraspinal pathways were protected by the treatments as revealed by retrograde tracing. Basso Beattie Bresnahan locomotor rating scores also revealed a measurable behavioral improvement. However, no evident behavioral improvements in locomotor performance were achieved by the combined treatment with annexin II and Reg-2, compared with the separate treatment with annexin II and Reg-2.
Assuntos
Anexina A2/uso terapêutico , Anti-Inflamatórios/uso terapêutico , Litostatina/uso terapêutico , Fatores de Crescimento Neural/uso terapêutico , Traumatismos da Medula Espinal/tratamento farmacológico , Animais , Antígenos CD/metabolismo , Modelos Animais de Doenças , Quimioterapia Combinada/métodos , Feminino , Proteína GAP-43/metabolismo , Regulação da Expressão Gênica/efeitos dos fármacos , Proteína Glial Fibrilar Ácida/metabolismo , Fator Estimulador de Colônias de Granulócitos/metabolismo , Interleucina-3/metabolismo , Locomoção/efeitos dos fármacos , Proteínas de Neurofilamentos/metabolismo , Ratos , Ratos Sprague-Dawley , Proteínas Recombinantes de Fusão/metabolismo , Proteínas Recombinantes , Traumatismos da Medula Espinal/fisiopatologia , EstilbamidinasRESUMO
To study the changes of somatostatin (SST) in frontal cortex of the brain of quick streptozotocin-induced diabetes rats. We established the quick-diabetic rats model by injecting streptozotocin intraperitoneally with 60 mg/kg and compared the changes of SST in frontal cortex of diabetic model with normal rats by using in situ hybridization and computer image analysis. The number, light density and the average area of positive cells in frontal cortex of diabetic model were reduced significantly in comparison with those of normal rats (P < 0.01). These results indicated that the decline of SST mRNA expression in frontal cortex may relate to chronic diabetes encephalopathy.
Assuntos
Diabetes Mellitus Experimental/metabolismo , Lobo Frontal/metabolismo , Somatostatina/biossíntese , Animais , Processamento de Imagem Assistida por Computador , Hibridização In Situ , Masculino , Neurônios/metabolismo , RNA Mensageiro/biossíntese , RNA Mensageiro/genética , Ratos , Somatostatina/genéticaRESUMO
OBJECTIVE: To investigate the relationship between diabetes and somatostatin (SOM). METHODS: We established a streptozocin-induced diabetic rats model and studied the changes of SOM mRNA in hippocampi of diabetic and normal rats by using in situ hybridication and computer image analysis. We studied the mechanism of chronic diabetes encephalopathy by observing the changes of somatostatin mRNA in the hippocampus of diabetic rats was studied. RESULT: The number, light density and average area of positive cells in dentate gyrus area of hippocampus in diabetes model were reduced significantly as compared with normal rats (P < 0.01). CONCLUSION: A decline in SOM mRNA expression may play a role in chronic diabetes encephalopathy because of SOM effect in brain.
