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1.
Mol Biol Rep ; 38(8): 5179-83, 2011 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-21225462

RESUMO

The objective of the present study was to identify polymorphisms of the CACNA2D1 gene, and to analyze associations between these polymorphisms and mastitis in several cattle breeds. Through PCR-RFLP methods and DNA sequencing, an allelic variant corresponding to the A→G mutations and Aspartic (Asp) to Glycine (Gly) amino acid replacement at positions 526745 in the exon 25 of bovine CACNA2D1 gene could be detected. Two alleles, A and G, and three genotypes, AA, AG and GG were defined. Genetic character in the studied populations indicated that the A526745G loci of CACNA2D1 gene was moderate polymorphism and fitted with Hardy-Weinberg equilibrium (P > 0.05). The effects of CACNA2D1 polymorphisms on somatic cell score (SCS) were analyzed and significant association was found between A526745G and SCS. The mean of genotype GG was significantly lower than those of genotype AG and AA (P = 0.0469). Information provided in this research could be useful in further studies to determine the role of CACNA2D1 gene in the mastitis resistance.


Assuntos
Canais de Cálcio/genética , Bovinos/genética , Estudos de Associação Genética , Leite/citologia , Polimorfismo de Nucleotídeo Único/genética , Animais , Cruzamento , Frequência do Gene/genética , Loci Gênicos , Genótipo
2.
Mol Biol Rep ; 38(1): 365-70, 2011 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-20585886

RESUMO

Calcium channel, voltage-dependent, alpha-2/delta subunit 1 (CACNA2D1) gene encodes a member of the alpha-2/delta subunit family, proteins are accessory molecules associated with voltage-gated calcium channels, and increase the density at the plasma membrane of calcium channels activated by high voltage. The main objective of the present study was to identify polymorphisms of CACNA2D1 gene, and to analyze associations between these polymorphisms and carcass and meat quality traits in cattle. In this study, through PCR-SSCP and DNA sequencing methods, two new allelic variant corresponding to the C→G and G→T mutations at positions 526740 and 537917 in the exon25 and exon35 of bovine CACNA2D1 gene, respectively, could be detected. SNP C526740G is a nonsynonymous mutation, resulting in a Cysteine (Cys) to Tryptophan (Trp) amino acid replacement and SNP G537917T resulting in an Aspartic (Asp) to Tyrosine (Tyr) amino acid replacement. The gene-specific SNP markers association analysis was investigated. The C526740G was significantly associated with Meat color (MC) (P=0.0297) and Backfat thickness (BF) (P<0.001). The G537917A indicated significant association with Dressing percentage (DP) (P=0.0485). No significant association, however, was detected between any of the marker genotype and other traits measured in this study. Results from this study initially suggested that CACNA2D1 gene is one of the potential candidate genes influencing carcass and meat quality traits and gene-specific SNPs may be a useful marker for MAS programs in cattle breeding.


Assuntos
Canais de Cálcio/genética , Bovinos/genética , Estudos de Associação Genética , Carne/normas , Polimorfismo de Nucleotídeo Único/genética , Característica Quantitativa Herdável , Animais , Cruzamento , Genótipo , Carne/economia , Reação em Cadeia da Polimerase , Polimorfismo Conformacional de Fita Simples
3.
Mol Biol Rep ; 38(7): 4705-8, 2011 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-21153925

RESUMO

Thyroid hormones play an important role in regulating metabolism and can affect homeostasis of fat deposition. The gene encoding thyroglobulin (TG), producing the precursor for thyroid hormones, has been proposed as a positional and functional candidate gene for a QTL with an effect on fat deposition. In the present study, we identified 6 novel SNPs at the 3' flanking region of the TG gene. The SNP marker association analysis indicated that the T354C, G392A, A430G and T433G SNP markers were significantly associated with marbling score (P < 0.05). Animals with the new homozygote genotype had higher marbling score than those with the other genotypes. Otherwise, the linkage disequilibrium analysis indicated that these four SNPs were completely linked (r (2) = 1). Results from this study suggest that TG gene-specific SNP may be a useful marker for meat quality traits in future marker assisted selection programs in beef cattle.


Assuntos
Bovinos/genética , Estudos de Associação Genética , Carne/normas , Polimorfismo de Nucleotídeo Único/genética , Característica Quantitativa Herdável , Tireoglobulina/genética , Animais , Sequência de Bases , Frequência do Gene/genética , Marcadores Genéticos , Genótipo , Dados de Sequência Molecular , Fenótipo
4.
Biochem Genet ; 48(9-10): 751-9, 2010 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-20549332

RESUMO

The objective of this study was to identify genetic polymorphisms of the CACNA2D1 gene and to analyze associations between SNPs and carcass and meat quality traits in cattle. Through PCR-RFLP and DNA sequencing methods, a new allelic variant corresponding to the A --> G mutation (aspartic to glycine amino acid replacement) of the bovine CACNA2D1 gene was detected. Two alleles and three genotypes (AA, AG, and GG) were defined. Genetic character indicated that the A526745G locus showed moderate polymorphism and was in Hardy-Weinberg equilibrium. Gene-specific SNP marker association analysis showed that the A526745G mutant was significantly associated with carcass weight, dressing percentage, meat percentage, and backfat thickness. The results add new evidence that CACNA2D1 is an important candidate gene for the selection of carcass and meat quality traits in the cattle industry.


Assuntos
Canais de Cálcio/genética , Bovinos/anatomia & histologia , Bovinos/genética , Carne/normas , Polimorfismo de Nucleotídeo Único , Animais , Cruzamento , Frequência do Gene , Marcadores Genéticos/genética , Genótipo
5.
Yi Chuan ; 30(3): 289-94, 2008 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-18331995

RESUMO

Diacylgycerol acyltransferase2 (DGAT2) is an important enzyme in many organisms. DGAT2 catalyzes the final step of triacylglycerol (TAG) biosynthesis by converting diacylgycerol (DAG) and fatty acyl-coenzyme A (CoA) into TAG. This enzyme is encoded by both DGAT2 and DGAT1 genes. This paper reviews the discovery of the DGAT2 gene, its structure, chromosomal location and biological effect. The relationship between its genetic polymorphisms and animal performance traits is also discussed. The review ends with future prospects.


Assuntos
Diacilglicerol O-Aciltransferase/genética , Diacilglicerol O-Aciltransferase/fisiologia , Animais , Diacilglicerol O-Aciltransferase/metabolismo , Humanos , Polimorfismo Genético/genética , Triglicerídeos/metabolismo
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