Comparison of three digestion methods for microplastic extraction from aquaculture feeds.

Microplastics (MPs) are ubiquitous pollutants found in aquaculture animals that may threaten human health through the food chain. However, there is a lack of effective methods for extracting MPs from aquaculture feeds containing complex components such as organic matter and fish bones. Therefore, in the present study, the extraction efficiency of three digestion methods using 30 % H2O2, Fenton reagent, and 30 % H2O2 + HNO3 for different particle sizes and types of MPs in aquaculture feeds was investigated and compared. The total digestion efficiency of the aquaculture feeds by 30 % H2O2 was 97.3 ± 0.1 %, while the recovery efficiency of MPs was 91.3 ± 1.1 % -103.1 ± 0.9 %. However, there was a large deviation in the extraction efficiency of MPs from aquaculture feeds by the Fenton reagent and 30 % H2O2 + HNO3. Notably, the surface morphology, particle size distribution, and oxidation degree of MPs hardly changed after 30 % H2O2 digestion. More importantly, the changes in the spectral features and carbonyl index of MPs after 30 % H2O2 digestion were smaller than those of the Fenton reagent and 30 % H2O2 + HNO3, which did not affect the identification of MPs. Overall, 30 % H2O2 was more efficient in extracting MPs from aquaculture feeds, and no significant effect on the characteristics of MPs was observed. This work provides novel insights into the effect of chemical pretreatment on the extraction of MPs in aquaculture feeds and provides an optimal protocol for the detection of MPs in aquaculture feeds.

Effect of long-term exposure to dyeing wastewater treatment plant effluent on growth and gut microbiota of adult zebrafish (Danio rerio).

Strict standards have been put forward for the treatment and discharge of dyeing wastewater worldwide. However, there are still traces amount of pollutants, especially emerging pollutants in dyeing wastewater treatment plant (DWTP) effluent. Few studies have focused on the chronic biological toxicity effect and mechanism of DWTP effluent. In this study, 3-month chronic compound toxic effects were investigated by the exposure of DWTP effluent using adult zebrafish. Significantly higher mortality and fatness and significantly lower body weight and body length were found in the treatment group. In addition, long-term exposure to DWTP effluent also obviously reduced liver-body weight ratio of zebrafish, causing abnormal liver development of zebrafish. Moreover, DWTP effluent led to obvious changes in the gut microbiota and microbial diversity of zebrafish. At phylum level, significantly higher of Verrucomicrobia but lower Tenericutes, Actinobacteria, and Chloroflexi were found in the control group. At genus level, the treatment group had significantly higher abundance of Lactobacillus, but significantly lower abundance of Akkermansia, Prevotella, Bacteroides, and Sutterella. These results suggested that long-term exposure to DWTP effluent led to imbalance of gut microbiota in zebrafish. In general, this research indicated that DWTP effluent pollutants could result in negative health outcomes to aquatic organisms.

Acute effects of antimony exposure on adult zebrafish (Danio rerio): From an oxidative stress and intestinal microbiota perspective.

The rapid development of the textile industry has resulted in a large influx of wastewater production. The "national discharge standards of water pollutants for dyeing and finishing of textile industry (GB4287-2012)" stipulates that the discharge of total Sb from textile industry effluent must be < 0.10 mg/L, but it is difficult to meet the standard at present. Antimony is potentially carcinogenic, and the pathogenic mechanism of antimony is poorly understood. In this study, the acute toxic effects of various concentrations of antimony on adult zebrafish (Danio rerio) were investigated, including effects on oxidative stress, neurotransmitters and intestinal microbiota. The activities of catalase (CAT), glutathione peroxidase (GSH-Px), malondialdehyde (MDA), superoxide dismutase (SOD), total antioxidant capacity (T-AOC) and acetylcholinesterase (AChE) were measured in zebrafish muscle and intestine tissue samples. In addition, intestinal microbial community composition and diversity of zebrafish were also analyzed. The results demonstrated that SOD, CAT and GSH-Px activities in the zebrafish gut showed a decreasing and then increasing trend with antimony concentration increasing. SOD, CAT and MDA in zebrafish muscle decreased with increasing exposure time. GSH-Px activities increased with increasing exposure time. T-AOC increased and then decreased. In addition, antimony exposure was neurotoxic to zebrafish, and a significant decrease in AChE activity was found in the intestine with increased exposure time. The neurotoxicity caused by antimony in the high concentration group (40 mg/L) was stronger than that in low concentration groups (10 mg/L and 20 mg/L). Notably, antimony exposure caused increases in the relative abundance of phyla Fusobacteriota and Actinomycetes, but decreases in the relative abundance of the phyla Firmicutes and Proteobacteria in zebrafish intestine. These outcomes will advance our understanding of antimony-induced biotoxicity, environmental problems, and health hazards. In conclusion, this study shows that acute exposure of antimony to zebrafish induces host oxidative stress and neurotoxicity, dysregulates the intestinal microbiota, showing adverse effects on the health and gut microbiota of zebrafish.

Biomechanical behavior of mandible with posterior marginal resection using finite element analysis.

This study aims to characterize biomechanical behavior of various designs of posterior mandibular marginal resection under functional loadings using finite element method. The ultimate goal of this work is to provide clinically relevant information to prevent postoperative fracture and to stipulate prophylactic internal fixation for planning of marginal mandibulectomy. A 3D mandibular master model was reconstructed from cone beam computed tomography images. Different marginal resection models were created based on three design parameters, namely, defect curvilinear radius, anterior-posterior defect width and residual height of the mandibular body. Functional loadings from incisors (60 N) and contralateral first molar area (200 N) were applied to designed models and stress patterns were compared of five groups with curvilinear radius from 0 (conventional rectangular shape), 2.5, 3.5, 5, and 6 mm. Models with 25, 35 and 45 mm defect width mimic defects varied from canine to 3rd molar were tested. Residual height range from 10 to 4 mm was assessed. The results show high stresses predominated in the occlusal area and the posterior inferior border near the resection corner. The average maximum stress decreased by 29.8% (r = 2.5 mm), 51.9% (r = 3.5 mm), 54.4% (r = 5 mm), and 59.3% (r = 6 mm) compared to the baseline of r = 0 mm. The results from the combined defect width/residual height models demonstrate the increase of defect width and the decrease in residual height resulted in the increase of maximum stress. Our data also confirm that the factor of residual height supersedes defect width in terms of prevention of postoperative fracture when considering resection design.

Effect of Chronic Exposure to Textile Wastewater Treatment Plant Effluents on Growth Performance, Oxidative Stress, and Intestinal Microbiota in Adult Zebrafish (Danio rerio).

The ever-increasing production and processing of textiles will lead to greater risks of releasing pollutants into the environment. Textile wastewater treatment plants (TWTPs) effluent are an important source of persistent toxic pollutants in receiving water bodies. The effects of specific pollutants on organisms are usually studied under laboratory conditions, and therefore, comprehensive results are not obtained regarding the chronic combined effects of pollutants under aquatic environmental conditions. Thus, this study aimed to determine the combined effects of TWTP effluents on the growth performance, oxidative stress, inflammatory response, and intestinal microbiota of adult zebrafish (Danio rerio). Exposure to TWTP effluents significantly inhibited growth, exacerbated the condition factor, and increased the mortality of adult zebrafish. Moreover, markedly decreases were observed in the activities of antioxidant enzymes, such as CAT, GSH, GSH-Px, MDA, SOD, and T-AOC, mostly in the intestine and muscle tissues of zebrafish after 1 and 4 months of exposure. In addition, the results demonstrated that TWTP effluent exposure affected the intestinal microbial community composition and decreased community diversity. Slight changes were found in the relative abundance of probiotic Lactobacillus, Akkermansia, and Lactococcus in zebrafish guts after chronic TWTP effluent exposure. The chronic toxic effects of slight increases in opportunistic pathogens, such as Mycoplasma, Stenotrophomonas, and Vibrio, deserve further attention. Our results reveal that TWTP effluent exposure poses potential health risks to aquatic organisms through growth inhibition, oxidative stress impairment of the intestine and muscles, and intestinal microbial community alterations.