RESUMO
A novel method for the determination of N-acetylneuraminic acid (NANA) and N-glycolylneuraminic acid (NGNA) was developed by using high-performance capillary electrophoresis (HPCE) with UV detection at 195 nm. NANA and NGNA were separated directly and analyzed without pre- or postcolumn derivation. The detection limit of NANA is 9.6 x 10(-6) mol L(-1) and for mass 3.879 x 10(-14) mol (39 fmol). This method was applied for the determination of NANA in 30 normal human and 72 cancer patients. The results demonstrated that NANA in the sera of cancer patients increased significantly as compared with the normal human (P < 0.001). The new method is simple and sensitive, and is suitable for basic research and clinical application to malignant tumors.
Assuntos
Biomarcadores Tumorais/sangue , Eletroforese Capilar/métodos , Ácido N-Acetilneuramínico/sangue , Neoplasias/sangue , Ácidos Neuramínicos/sangue , Adulto , Análise Química do Sangue/métodos , Eletroforese Capilar/instrumentação , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Valores de ReferênciaRESUMO
Yangyin Anshen Koufuye could obviously decrease the spontaneous activity of rats, effectively shorten the process of falling into sleep of normal rats which were injected pentobarbital sodium and prolong the sleeping time. The effect of sedation and hypnotism were the same as Zhaoren Anshen Koufuye. Yangyin Anshen Koufuye also could markedly reduce the serum tensity of T3, T4 of hyperthyroid rats, which equaled to TCM's deficiency of yin. It also could prevent the glycogen content of liver from decreasing, decrease heart rate, resist weight losing, thus to show the effect of nourishing Yin and tranquilizing.
Assuntos
Medicamentos de Ervas Chinesas/farmacologia , Hipertireoidismo/metabolismo , Plantas Medicinais , Deficiência da Energia Yin/metabolismo , Animais , Comportamento Animal/efeitos dos fármacos , Combinação de Medicamentos , Medicamentos de Ervas Chinesas/isolamento & purificação , Feminino , Glicogênio/metabolismo , Fígado/metabolismo , Masculino , Camundongos , Fitoterapia , Plantas Medicinais/química , Ratos , Ratos Sprague-Dawley , Rehmannia/química , Tiroxina/metabolismo , Tri-Iodotironina/metabolismo , Ziziphus/químicaRESUMO
A pathogen was isolated from natural dead pests of Ceracris kiangsu in Geleshan farm of Chongqin. Its pathogenecity was confirmed by the law of KOCK. It was identified as Pseudomonas pseudoalcaligenes according to its physiological and biochemical properties as well as the G + C content of DNA (63.73mol%). The results of preliminary bioassay show that the pathogen can infect the grasshoppers and Ceracris kiangsu, and also can infect other pests of grassland in a certain extent.
