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BACKGROUND: Changes of leukocytes are related with some postoperative complications after renal transplantation. OBJECTIVE: To observe dynamically the level of white blood cell (WBC) and its morphological changes using automatic blood cell analyzer to evaluate its relationship with infection, immunosuppressive intoxation and acute rejection. DESIGN, TIME AND SETTING: Observation and controlled trial was performed at Second Xiangya Hospital, Central South University from June 2004 to October 2005. PARTICIPANTS: Sixty-eight patients who received renal transplantation were selected. According to clinical symptoms and laboratory biochemical indexes, X-ray, B ultrasound, and renal puncture biopsy, the patients were divided into stable group (n=36), infection group (n=9), intoxation group (n=6) and acute rejection group (n=13). METHODS: The blood routine was tested with Sysmex XE-2100 automatic blood analyzer following renal transplantation and WBC manual differentiation was done to observe peripheral blood morphology, their liver function. In addition, renal and liver functions were detected by automatic biochemical system 7600A. MAIN OUTCOME MEASURES: WBC count and its morphological changes in peripheral blood and liver and renal function after renal transplantation. RESULTS: WBC count of the stable group and intoxation group was normal, and that of the acute rejection group and infection group were significantly higher than the stable group (P
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0.77). The average intra lot coefficients of variation (CV) of HPC of high, medium and low value was
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OBJECTIVE To investigate the distribution of serum markers of transfusion-transmitted infections in hospital,and analyze the clinical values in the control of nosocomical infection. METHODS The serum markers of transfusion-transmitted infections from 21 605 hospitalized cases,such as HBsAg,anti-HCV,anti-HIV,and RPR were detected. RESULTS The positive rate of HBsAg and anti-HCV was 9.78% and 1.70%,respectively.The positive anti-HIV was detected in 13 cases by the primary screening test for HIV,and the 13 cases were reconfirmed by the reconfirmation laboratory of CDC in Hunan Province.The positive RPR was in 4 cases,the same as of TPHA.There were 2 582 positive cases of the four indexes(HBsAg,anti-HCV,anti-HIV and RPR),the total positively detectable rate was 11.95%,which was obviously higher than 7.6% of normals(P