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Nicotinamide phosphoribosyltransferase( NAMPT) is the rate-limiting enzyme that catalyzes the first step in Nicotinamide adenine dinucleotide( NAD) biosynthesis and regulates biological processes such as metab-olism,growth and apoptosis of cells.In the past few years,a large number of studies have found that expression level of NAMPT is increased in a variety of malignancies,which is associated with the progression and treatment of malig-nant tumors.The research of the mechanism of NAMPT in malignant tumors and molecule inhibitors of the NAMPT have brought new hope and prospect for the treatment of malignant tumors.This article makes a brief review of NAMPT research status in malignant tumors.
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Objective To explore the influence of renal insufficiency with microwave therapy in the treatment of liver cancer .Methods A retrospective clinical analysis with 80 patients who underwent microwave therapy of liver cancer ,was performed from June 2012 to March 2014 in the department of hepatobiliary and pan-creatic surgery ,the Affiliated Tumor Hospital of Harbin Medical University .According to perioperative presence of renal insufficiency,we divided the patients into renal dysfunction group (A)(n=44)and normal renal function group(B)(n=36);Preoperative ALT,TBIL,BUN,intraoperative hypotension,intraoperative urinary volume,age, the distance between the tumor and the main vessels in the liver ,postoperative microwave ablation time the differ-ences of the risk factors ,such as microwave ablation range and sex of the two groups were compared during micro -wave therapy .Results There was no statistical significant difference between the two groups on intraoperative u -rinary volume(P>0.05);The preoperative ALT,TBIL,BUN,intraoperative hypotension,age,microwave ablation time,the distance between the tumor and the main vessels in the liver ,scular microwave ablation range ,sex of two groups showed statistical differences (P<0.05).BUN with preoperative ALT,TBIL,preoperative BUN,intraoper-ative hypotension,age,the distance between the tumor and the main vessels in the liver ,time of microwave abla-tion,microwave ablation range,sex influence factors were positively correlated (r=0.63,0.60,0.58,0.49,0.45, 0.40,0.35,0.46,0.52,P<0.05).Strength and influencing factors of BUN are the distance between the tumor and the main vessels in the liver,microwave ablation range,intraoperative hypotension,preoperative ALT,sex,pre-operative TBIL,microwave ablation time,age,preoperative BUN(β=0.52,0.42,0.37,0.31,0.29,0.25,0.20, 0.18,0.16,P<0.05).Conclusion Microwave therapy leads to renal insufficiency in the treatment of liver cancer performs many aspects , including preoperative ALT , TBIL, preoperative preoperative BUN , intraoperative hypotension ,age,the distance between the tumor and the main vessels in the liver ,time of microwave ablation ,mi-crowave ablation range ,sex,which is closely related to the factors affecting the risk of kidney .
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The single or combined administration of Raltitrexed with other anticancer drugs have showed prospective curative effects on solid tumor .This paper reviews the current research situation of Raltitrexed against malignant tumor and explores the possibilities for the efficacy and mechanism in treatment of malignant tumor . Raltitrexed is one kind of effective anti cancer drugs and plays a more and more important role in solid tumor ther -apy.
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Objective To test and compare CA19-9,CEA with Specific N-Glycans in early-middle stage pancreatic carcinoma serum to find a better tumor marker in early pancreatic carcinoma .Methods To find the difference of N-Glycans structure ,DSA-FACE was performed in 35 early middle stage pancreatic carcinoma patients and 50 healthy human .Meanwhile ,we tested CA19-9 and CEA in the pancreatic carcinoma patients at the same stage.Result The serum N -glycan profiles of pancreatic carcinoma was identified by the DSA -FACE technique.The results showed that between pancreatic carcinoma patients and healthy persons ,there were significant differences in N -glycans.The peak 13,14 and 17 were the most significant peaks which would be most likely picked as a new tumor marker of pancreatic carcinoma .Taking log(p14 ×p17/p13)as indicator of the ROC curve analysis,and the area under ROC curve was 0.799 ±0.050 with 84.9% sensitivity and 68% speci-ficity.Meanwhile,the sensitivity of CA19-9 was 61.2%,the sensitivity of CEA was 11.7%.Conclusion The Peak 13,14 and 17 are the most significant peaks which would be picked as a new tumor marker of pancreatic carcinoma.Espicially,its sensitivity is superior to CA19-9、CEA for early middle stage patients .
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Objective To investigate the structural changes in specific serum N-glycans in pancreatic cancer patients and to identify the specific serum maker of pancreatic cancer.Methods The pancreatic cancer patients who visited the Third Affiliated Hospital of Harbin Medical University from June 201 1 to December 2013 were assigned to preoperative serum group (123 cases)and postoperative serum group (78 cases);healthy controls whose serum samples were collected in the Physical Examination Center were selected as control serum group (271 cases).DNA sequencer-aided fluorophore-assisted carbohydrate electrophoresis (DSA-FACE)was used to analyze serum N -glycans and compare them between the three groups.Results The serum N-glycan profiles in pancreatic cancer patients were identified by DSA-FACE.The results indicated that N-glycan peak 8 in preoperative serum group was significantly lower than those in control serum group (t=2.735,P<0.05)and postoperative serum group (P<0.05),but no significant difference was found between the postoperative serum group and control serum group.Conclusion N-glycan peak 8 can be considered as a serum marker of pancreatic cancer.
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Objective To investigate the apoptosis induced by adenovirus mediated PML growth suppressor in SGC-7901 gastric cancer cells. Methods Human full-length PML cDNA was cloned into shuttle plasmid pSGCMV, the constructed plasmid containing PML gene was co-transfected into 293 cells together with backbone plasmid pPE3 to obtain recombinant adenovirus Ad-pml. Ad-pml was used to infect SGC-7901 cells. The expression of p53 and bcl-2 in SGC-7901 cells and qualitative, quantitative index of cell apoptosis were detected with MTT, flow cytometry and immunohistochemical method. Results The growth of SGC-7901 cells was inhibited and the apoptosis rate was increased by adenovirus mediated PML in a MOI- dependent manner. The expression of p53 protein was increased and that of bcl-2 protein was unchanged in SGC-7901 cells after treated with adenovirus mediated PML. Conclusions Adenovirus mediated PML exerts its cytotoxic effect on SGC-7901 cells by inducing apoptosis. The increase of p53 protein expression is the mechanism inducing gastric cancer cell apoptosis.
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Objective To summarize our experience on the diagnosis and treatment of gastric smooth muscle tumor.MethodThe clinical data of 34 cases with gastric smooth muscle tumor were analyzed retrospectively.ResultLeiomyosarcomas were ≥5 cm in diameter in 68% of all cases, and ≥10 cm in 50%. Most leiomyosarcomas were located in the fundus and corpus of the stomach (94%). Some patients of gastric leiomyosarcoma have the triad of epigastric pain, mass, and bleeding. Gastroscopy examination often reveals a mass lying submucously. Total or partial gastrectomy was performed for all patients based on tumor size and locus. The 3, 5, 10-year′s survival rate were 87%, 52% and 26% respectively. Conclusion Most gastric leiomyosarcomas are characteristic of local invasiveness, postoperative survival is often favourable.
