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Objective To establish a carrying system for space cellular experiment suitable for astronaut to carry out cellular experiments on Shenzhou-6 mission.Methods The cell carrying sample bag,sample box and sample box integrated package were designed.Primary cardiomyocytes and osteoblasts culture and ground model experiment in the simulated environment of space cabin were performed.With man-tended,the cellular experiment was carried out on the orbit.Results After 5 d space flight,the returned cell samples were analyzed.The results demonstrated that the system was of good safety,reliability and applicability,as well as satisfied the demands of analyzed samples.Conclusion After Shenzhou-6 space flight,it is showed that this system fits for small loading,multi-cells and man-tended carrying mission,and can satisfy the demand of the first man-tended space cellular experiments carried out on the Shenzhou-6 spacecraft.
RESUMO
UNLABELLED: It has been documented that astronauts suffer from a progressive and continuous negative calcium balance in space flight. The aim of this trial was to demonstrate the role of force incentive on protection of bone lose in stimulated microgravity rats. MATERIALS AND METHODS: 7-week-old male Wistar rats were randomly divided into 3 groups. One group was suspended by tail for 21 days, one groups were suspended for 21 days and play 1 minutes/day of force incentive (2N, 10 Hz), one group served as control. bone mass, biomechanical properties, histomorphometry and biochemical marked were determined. Results Short-time force incentive not only can improve the metabolism of calcium and phosphorus as well as histomorphometry of tibia, but also can increase bone mineral, biomechanical properties and decrease the content of NO in femur. Conclusion Short-time force incentive can protect the bone lose in stimulated microgravity. Compare with the traditional methods such as using drug, the physical methods, for example, short-time force incentive are less vehement and more safety, which can maintain the normal structure of bone. So short-time force incentive maybe can develop to the primary protective method on osteoporosis.
