Effect of Bifidobacterium lactis M8 on immune function of immunosuppressive model rats / 吉林大学学报(医学版)

Objective: To investigate the percentages of lymphocyte subsets∗ the transformation and proliferation activities of T lymphocytes∗ the expression levels of serum immunoglobulin and cytokines in the immunosuppressive model rats after given Bifidobacterium lactis M8, and to elucidate the effect of Bifidobacterium lactis M8 on the immune function of the immunosuppressive model rats. Methods: Fifty Wistar rats were randomly divided into blank control group, model control group, low dose of Bifidobacterium lactis M8 group, middle dose of Bifidobacterium lactis M8 group, and high dose of Bifidobacterium lactis M8 group, and there were 10 rats in each group. The rats in each group were administrated with probiotic bifidobacterium lactis M8 for 6 d. From the 7th day∗ except for blank control group∗ the rats in the other groups were given cyclophosphamide (CTX) for 3 d to construct the immunosuppressive models; the rats in low, middle∗ and high doses of Bifidobacterium lactis M8 groups were given 0. 01, 0. 10 and 1. 00 g bifidobacterium lactis M8» and the rats in blank control group and model control group were given sterile saline with the same volume. The percentages of lymphocyte subsets (CD3 + T lymphocytes, CD4 + T lymphocytes, CDS + T lymphocytes, B lymphocytes and NK cells) in whole blood of the rats in various groups were detected by flow cytometry, the transformation and proliferation activities of T lymphocytes of the rats in various groups were detected by CCK-8 method, the levels of serum immunoglobulin A (IGA), immunoglobulin G (IgG), interleukin-2 (IL-2), interleukin-6 (IL-6), interferon-y (IFN-y) and tumor necrosis factor-a (TNF-a) of the rats in various groups were detected by ELISA method. Results: Compared with blank control group, the percentages of CD3 + T lymphocytes, CD4 + T lymphocytes∗ CDS + T lymphocytes, B lymphocytes and NK cells in whole blood of the rats inmodel control group were decreased significantly ( P'<0. 05); compared with model control group, the percentages of CD3 + T lymphocytes, CD4 + T lymphocytes and CDS + T lymphocytes in whole blood of the rats in high dose of Bt/idobacterium lactis MS group were increased significantly ( P<0. 05 or P

Biomechanical Analysis and Calculation of Lenke1A/B Type Scoliosis Correction / 医用生物力学

Objective The functional relation between the correction angle and orthopedic force of scoliosis can be derived based on the force principle of scoliosis correction. The position and size of the orthopedic force with the best effect of spinal correction can be calculated to provide a theoretical calculation method for the application of orthopedic forces on scoliosis. Methods The coordinates of the scoliosis central axis were collected based on the computed tomography (CT) data of scoliosis. The spline curve of scoliosis was fitted using the MATLAB software. The scoliosis constitutive polynomial was obtained, and then, the scoliosis Cobb angle was calculated by programming. Based on the clinical medicine and experimental data of spinal biomechanics, a curved beam model was used for the correction calculation and analysis of scoliosis. Results The function relation between the orthopedic force and angle at any positions was derived at different orthopedic forces. The comprehensive elastic modulus of the spine was 0.29 MPa, as calculated by the function relation. Conclusions The calculation and analysis provided a reliable theoretical basis for the relationship between the loading position, size, and restoration angle of the orthopedic forces during scoliosis correction. For patients with different types of scoliosis, the function relation between the orthopedic angle and orthopedic force could be optimized to obtain the optimal orthopedic force and loading position.

Long-term amelioration of telmisartan on metabolic syndrome-related molecules in stroke-resistant spontaneously hypertensive rat after transient middle cerebral artery occlusion.

Telmisartan is expected to ameliorate not only hypertension, but also metabolic syndrome as a metabosartan. We examined the effects of telmisartan on metabolic syndrome-related molecules such as insulin receptor (IR), peroxisome proliferator-activated receptor gamma (PPAR-γ), and angiotensin 2 type 1 receptor (AT1R) in stroke-resistant spontaneously hypertensive rat (SHR-SR) after transient middle cerebral artery occlusion (tMCAO), by administering telmisartan at either 0 (vehicle), .3 mg/kg/day (low dose), or 3 mg/kg/day (high dose), postoperatively, from 3 months of age and performed immunohistologic analysis at 6, 12, and 18 months of age. Compared with the vehicle group, the 2 telmisartan groups dose dependently decreased the number of IR- and AT1R-positive neurons in the cerebral cortex in the ipsilateral cerebral cortex from 6 to 18 months after tMCAO. On the other hand, the number of PPAR-γ-positive neurons increased in a dose-dependent manner in the 2 telmisartan groups from 6 to 18 months. The present study suggests that telmisartan dose-dependently ameliorated metabolic syndrome-related changes in the poststroke brain of SHR-SR with a direct protective effect (low dose) and an additive benefit, an antihypertensive effect at a high dose, for long-term protection after tMCAO.

Effect of HSP70 on apoptotic of cartilage cells in knee osteoarthritis / 中国骨伤

<p><b>OBJECTIVE</b>To investigate the expression and relationship between HSP70 and caspase-3 in knee osteoarthritis.</p><p><b>METHODS</b>Forty adult SD rats were divided into experimental group and control group. Thirty rats in experimental group, anterior cruciate ligament transaction (ACLT) was cut off and partial meniscectomy of 1/3 inside incision were performed to reproduce knee osteoarthritis (KOA) model according to Hulth methos, and the other 10 rats was treated with nothing as control group. The rats were sent to the cage and free to move. At 1, 2 and 4 weeks later, the arthritis cartilage of femoral and tibial end were observed through immunohistochemistry staining and light microscope. Meanwhile, Mankin scale system was adopted for histomorphology evaluation.</p><p><b>RESULTS</b>Changes of KOA such as hyperplastic synovium,erosion on the surface of cartilage and so on were found in experiment group, the expression of HSP70 was augmentation all the time, but the expression of caspase-3 was reduction 1 week later; no similar changes were found in control group. Mankin scale system showed that there were significant differences in the first week as compared with the second week and 4th week (both P < 0.01).</p><p><b>CONCLUSION</b>Heat shock protein inhibit the apoptosis of cartilage cells and protect the cartilage cells in knee osteoarthritis, the conservative treatment for clinical provide objective scientific basis.</p>

Basic research progress of knee osteoarthritis / 中国骨伤

The exact etiology and pathogenesis of knee osteoarthritis (KOA) are still unknown and it is hard to treat the disease fundamentally. With new therapeutic methods and techniques appearing, the present situation of treating the disease will be changed in the near future. Basic research of knee osteoarthritis will contribute to clarifying the pathogenesis and exploring the therapeutic methods. This article makes a brief review on the up-to-date basic researches of knee osteoarthritis by reviewing literature concerned in recent years.

The significance of p16 protein and Ki-67 antigen expression in gastric mucosa-associated lymphoid tissue lymphoma / 中华内科杂志

To detect the expression of p16 protein and Ki-67 antigen in gastric mucosa-associated lymphoid tissue lymphoma(MALTL)and normal gastric mucosa and to investigate the clinical significance of their expression in the occurrence and development of gastric MALTL.Methods 47 samples of gastric MALTL diagnosed pathologically in the department of pathology of the PLA General Hospital from March 1993 to June 2005 were collected.By using immunohistochemial methods,the expression of p16 protein and Ki-67 antigen was detected in 20 samples of normal gastric tissue and the 47 samples of gastric MALTL.Results The positive rate of p16 protein Was 21.3%(10/47)and 90.0% in gastric MALTL and normal gastric tissue respectively.The positive rate of p16 protein in gastric MALTL was lower than that in normal gastric tissue(P<0.05).The expression of p16 was related to the degree of lymph node metastasis.The positive rate of Ki-67 labelling index(LI)in gnstric MALTL was hiigher than that in normal gastric tissue.A negative correlation was found between the expression of p16 protein and Ki-67 LI(P<0.05). Conclusions Detection p16 and Ki-67 may help to predict the possibility of lymph node metastasis and prognosis in gastric MALTL.

Analysis of the unsuccessful cases of the porcelain-fused-to-metal-crowns and bridges / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To discuss the unsuccessful reasons and the preventive methods of the unsuccessful clinic cases.</p><p><b>METHODS</b>The retrospective analysis was carried on the 80 prosthesis in 70 patients from 1994 to 2001 which were suffered with unsuccessful results after the restoration of the metal crowns and bridges.</p><p><b>RESULTS</b>There were 68 teeth which had collapsed or broken. 20 teeth had the post loosing or shedding. Root breaking, food impaction, unharmony colors of porcelain and the changing color of gingival were 3, 3, 6 and 2 teeth.</p><p><b>CONCLUSION</b>It is necessary to select suitable repairing materials and operate correctly for preventing the occurring of the unsuccessful results in the porcelain-fused-to-metal-crowns and bridges.</p>

Study on single nucleotide polymorphism of TLR4 in Chinese population / 中华医学遗传学杂志

<p><b>OBJECTIVE</b>To identify the single nucleotide polymorphisms(SNPs) in the regulatory and coding regions of human Toll-like receptor 4(TLR4) gene and to search for its new genetic makers.</p><p><b>METHODS</b>The 5' flank region, exons, parts of the introns, as well as 3' flank region of TLR4 gene were sequenced to identify and characterize the SNPs in Chinese population. SNP genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism for 2 highly distributed SNPs.</p><p><b>RESULTS</b>Five novel SNPs were identified through a 4.98 kb sequencing of TLR4 gene. Among them, three were in 5'flank region, two in 3'UTR. In the sample of Han population from Chongqing, the minor allele frequencies of two highly distributed SNPs were 0.266 and 0.404 respectively.</p><p><b>CONCLUSION</b>Sampling analysis in Han population of Chongqing showed that the two highly distributed SNPs of TLR4 were common in Chinese population and could be used for genetic marker of TLR4 gene.</p>

[Pondering acupuncture treatment on anxiety disorders].

Review of papers about acupuncture treatment on anxiety disorders since 1996 showed that many papers were mainly concerned with the treatment of anxiety disorders and obsession with electroacupuncture therapy. Baihui, Yintang, Shenmen, Dazhui, Taiyang, etc, were the most commonly involved acupoints. This paper argues that more emphasis should be transferred from "heart" to "brain", and correspondingly, should be put on acupoints at the head, the DU meridian and acupoints nearby in terms of the position of treatment, on scalp acupuncture and acupoint injection in terms of the methods of treatment, on the combination of diagnosis by western medicine and syndrome differentiation of traditional Chinese medicine in terms of treating strategy. As to the research method, evidence-based medicine, and multicenter, large sample randomized controlled trial study on anxiety disorders should be made.

An investigation of polymorphism in IL-1R1 gene in Chinese population and the functional prediction of a variation in the transmembrane region of IL-1R1 / 中华医学遗传学杂志

<p><b>OBJECTIVE</b>To identify the single nucleotide polymorphisms (SNPs) in the regulatory and coding regions of human interleukin-1 receptor type I (IL-1R1) gene and to assess their potential effect on the function of IL-1R1.</p><p><b>METHODS</b>The 5' flank region, exons, parts of the introns, as well as 3' flank region of IL-1R1 gene were sequenced to identify and characterize the SNPs in Chinese population. Effects of the SNP on the structure and function of IL-1R1 were analyzed by computational methods.</p><p><b>RESULTS</b>Sixteen SNPs were identified through a 9643 bp sequencing of IL-1R1 gene. Among them, four were in 5' flank region, four in intron region, one in coding region, and seven in 3' untranslated region. A novel SNP in Chinese population was involved in a structural change in IL-1R1, which may influence the signal transduction of IL-1R1.</p><p><b>CONCLUSION</b>The SNP in the IL-1R1 gene might influence its function as an important receptor of IL-1 family.</p>

Clinical study on 52 patients of post-stroke depressive disorder treated with traditional and western medicine / 中国康复理论与实践

@#ObjectiveTo evaluate the clinical effect of integrated traditional and western medicine in the treatment of post stroke depressive disorder (PSDD). MethodsThe data of 52 patients of PSDD evaluated on self rating depression scale(SDS) before and after treatment were analyzed statistically. ResultsThe curing rate of PSDD is about 78.85%.There was an apparent decrease in the level of SDS before and after treatment(P<0.001). ConclusionsThe treatment of integrated traditional Chinese and western medicine can produce a good effect on PSDD.