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Objective To compare antibiotic resistance of pseudomonas aeruginosa in biofilms and in planktonic state,so as to provide practical guidance and theoretical support for clinical treatment of drug. Methods pseudomonas aeruginosa were divided into control culture group( group A),medium group of biological membrane after culture( group B) and the biofilm culturing silica film adhesion group( group C). Three groups were observed by silver staining,extracellular sugar staining and scanning of electron microscope. These groups were cultured with different antibiotic medium,and the situation of bacterial resistance was observed by the method of k-B. Results There was no significant difference in drug resistance and minimum inhibitory concentration(MIC)between group A and B(p >0. 01). Compared with group A, group C showed that their drug resistance on meropenem,cefepime,ceftazidime,ceftriaxone,amoxicillin and levofloxacin were much higher(p<0. 01). The minimal biofilm eradication concentration(MBEC)of group C was about 100 times than that of group A and group B. Conclusion The assay of establishment of pseudomonas aeruginosa biofilm and the methods observed by silver stainingin vitro is convenient and feasible. At the same time,pseudomonas aeruginosa in biofilm exhibits far more resistance to antimicrobial than its planctonic counterparts does.
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OBJECTIVE@#To study the expression of E-cadherin, N-cadherin, TGF-β1 and Twist protein and investigate its significance in the occurrence and development of prostate cancer.@*METHODS@#The expression of E-cadherin, N-cadherin, TGF-β1 and Twist protein in 59 prostate cancer tissues and 21 adjacent tissues were detected by immunohistochemical SABC staining, and the correlation with clinicopathological features was analyzed.@*RESULTS@#Positive rates of E-cadherin, N-cadherin, TGF-β1 and Twist were 32.2%, 54.2%, 71.2% and 74.6%, respectively, in prostate cancer tissues and 85.7%, 9.52%, 19.0% and 9.52%, respectively, in cancer-adjacent tissues, with significant differences between the two groups (P20μg/L group, but the positive expression of Twist was not significant between groups. The expression of E-cadherin was highly negatively correlated with that of N-cadherin and also highly negatively correlated with that of Twist. The expression of TGF-β1 was correlated with those of E-cadherin, N-cadherin and Twist.@*CONCLUSIONS@#The reduced expression of E-cadherin, abnormal expression of N-cadherin, transformation form E-cadherin to N-cadherin and the increased expression of TGF-β1 and Twist play an important role in the occurrence and development of prostate cancer.
