RESUMO
Transgenic targeting of SV40 large T antigen (Tag) expression to murine cerebellar Purkinje cells induces these normally postmitotic neurons to undergo DNA synthesis and apoptosis. It has been proposed that these effects of Tag are due to the binding of Tag to pRb, which leads to the release and activation of the transcription factor E2F. Here it is reported that E2F and CDC2, the protein product of a gene regulated by E2F, were detectable in the Purkinje cell nuclei of Tag expressing transgenic animals. To directly test whether E2F-1 is part of the mechanism of Tag-induced Purkinje cell degeneration, transgenic mice that overexpress E2F-1 specifically in cerebellar Purkinje cells were generated. Although E2F-1 itself did not affect Purkinje cells, it did accelerate Tag-induced ataxia and Purkinje cell loss, suggesting that E2F-1 can contribute to the mechanism of Tag-induced Purkinje cell degeneration.
Assuntos
Antígenos Transformantes de Poliomavirus/genética , Proteínas de Transporte , Moléculas de Adesão Celular Neuronais , Proteínas de Ciclo Celular , Degeneração Neural/metabolismo , Células de Purkinje/fisiologia , Fatores de Transcrição/fisiologia , Animais , Antígenos Transformantes de Poliomavirus/metabolismo , Northern Blotting , Proteína Quinase CDC2/genética , Contactina 2 , Proteínas de Ligação a DNA/análise , Proteínas de Ligação a DNA/fisiologia , Fatores de Transcrição E2F , Fator de Transcrição E2F1 , Expressão Gênica/fisiologia , Glicoproteínas de Membrana/genética , Camundongos , Camundongos Transgênicos , Células de Purkinje/química , RNA Mensageiro/metabolismo , Proteína 1 de Ligação ao Retinoblastoma , Fator de Transcrição DP1 , Fatores de Transcrição/análiseRESUMO
Spinocerebellar ataxia type 1 (SCA1) is an autosomal dominant neurological disorder caused by the expansion of a CAG repeat encoding a polyglutamine tract. Work presented here describes the behavioral and neuropathological course seen in mutant SCA1 transgenic mice. Behavioral tests indicate that at 5 weeks of age mutant mice have an impaired performance on the rotating rod in the absence of deficits in balance and coordination. In contrast, these mutant SCA1 mice have an increased initial exploratory behavior. Thus, expression of the mutant SCA1 allele within cerebellar Purkinje cells has divergent effects on the motor behavior of juvenile animals: a compromise of rotating rod performance and a simultaneous enhancement of initial exploratory activity. With age, these animals develop incoordination with concomitant progressive Purkinje neuron dendritic and somatic atrophy but relatively little cell loss. Therefore, the eventual development of ataxia caused by the expression of a mutant SCA1 allele is not the result of cell death per se, but the result of cellular dysfunction and morphological alterations that occur before neuronal demise.
Assuntos
Comportamento Animal/fisiologia , Cerebelo/fisiopatologia , Camundongos Transgênicos/genética , Atividade Motora/fisiologia , Células de Purkinje/fisiologia , Degenerações Espinocerebelares/genética , Alelos , Animais , Contagem de Células , Cerebelo/patologia , Camundongos , Mutação , Sistema Nervoso/fisiopatologia , Células de Purkinje/patologia , Degenerações Espinocerebelares/patologia , Degenerações Espinocerebelares/fisiopatologiaRESUMO
Purkinje cells are uniquely susceptible to a number of physical, chemical, and genetic insults both during development and in the mature state. We have previously shown that when the postmitotic state of murine Purkinje cells is altered by inactivation of the retinoblastoma tumor susceptibility protein (pRb), immature as well as mature Purkinje cells undergo apoptosis. DNA synthesis and neuronal loss are induced in postmitotic Purkinje cells dependent upon the pRb-binding portion of SV40 large T antigen (T-ag). In the present study, Purkinje cell targeting of a mutant T-ag, PVU, which does not bind pRb, reveals disparate cerebellar phenotypes dependent upon temporal differences in transgene expression. Strong embryonic and postnatal transgene expression in three lines alters Purkinje cell development and function during the second postnatal week, causing ataxia without Purkinje cell loss. In contrast, two other transgenic lines reveal that PVU T-ag expression following normal Purkinje cell maturation causes rapid Purkinje cell degeneration. The second and third postnatal weeks of cerebellar development, which include the major period of synaptogenesis, appear to be the defining stage for the two PVU-induced phenotypes. These data indicate that Purkinje cell death susceptibility varies with developmental stage.
Assuntos
Antígenos Transformantes de Poliomavirus/farmacologia , Morte Celular/efeitos dos fármacos , Cerebelo/crescimento & desenvolvimento , Células de Purkinje/efeitos dos fármacos , Animais , Imuno-Histoquímica , Camundongos , Camundongos TransgênicosRESUMO
Transgenic FVB/N mice overexpressing human (Hu) or mouse (Mo) Alzheimer amyloid precursor protein (APP695) die early and develop a CNS disorder that includes neophobia and impaired spatial alternation, with diminished glucose utilization and astrogliosis mainly in the cerebrum. Age at onset of neophobia and age at death decrease with increasing levels of brain APP. HuAPP transgenes induce death much earlier than MoAPP transgenes expressed at similar levels. No extracellular amyloid was detected, indicating that some deleterious processes related to APP overexpression are dissociated from formation of amyloid. A similar clinical syndrome occurs spontaneously in approximately 20% of nontransgenic mice when they reach mid- to late-adult life, suggesting that APP overexpression may accelerate a naturally occurring age-related CNS disorder in FVB/N mice.
Assuntos
Doença de Alzheimer , Precursor de Proteína beta-Amiloide/genética , Doenças do Sistema Nervoso Central/genética , Expressão Gênica , Envelhecimento , Animais , Astrócitos/patologia , Sequência de Bases , Comportamento Animal , Encéfalo/metabolismo , Encéfalo/patologia , Doenças do Sistema Nervoso Central/mortalidade , Doenças do Sistema Nervoso Central/fisiopatologia , Cosmídeos , Feminino , Vetores Genéticos , Glucose/metabolismo , Humanos , Masculino , Camundongos , Camundongos Transgênicos , Dados de Sequência MolecularRESUMO
Spinocerebellar ataxia type 1 (SCA1) is an autosomal dominant inherited disorder characterized by degeneration of cerebellar Purkinje cells, spinocerebellar tracts, and selective brainstem neurons owing to the expansion of an unstable CAG trinucleotide repeat. To gain insight into the pathogenesis of the SCA1 mutation and the intergenerational stability of trinucleotide repeats in mice, we have generated transgenic mice expressing the human SCA1 gene with either a normal or an expanded CAG tract. Both transgenes were stable in parent to offspring transmissions. While all six transgenic lines expressing the unexpanded human SCA1 allele had normal Purkinje cells, transgenic animals from five of six lines with the expanded SCA1 allele developed ataxia and Purkinje cell degeneration. These data indicate that expanded CAG repeats expressed in Purkinje cells are sufficient to produce degeneration and ataxia and demonstrate that a mouse model can be established for neurodegeneration caused by CAG repeat expansions.
Assuntos
Modelos Animais de Doenças , Camundongos Transgênicos/genética , Sequências Repetitivas de Ácido Nucleico/genética , Degenerações Espinocerebelares/genética , Animais , Ataxina-1 , Ataxinas , Sequência de Bases , Cerebelo/patologia , Expressão Gênica/genética , Imuno-Histoquímica , Camundongos , Dados de Sequência Molecular , Degeneração Neural/genética , Proteínas do Tecido Nervoso/genética , Proteínas Nucleares/genética , Fenótipo , Células de Purkinje/fisiologia , RNA Mensageiro/análiseRESUMO
The product of the retinoblastoma susceptibility gene, pRb, is known to be an important regulator of cell division. Disrupted central nervous system development in RB null mice suggests a critical function for pRb in the proliferative arrest and initiation of terminal differentiation of certain neurons. Previously, we have shown that SV40 T-ag expression targeted to Purkinje neurons in transgenic mice causes cell-specific death. Here we describe that T-ag expression induces DNA synthesis and results in DNA fragmentation in Purkinje neurons. Characterization of transgenic mouse lines expressing mutant T-ags demonstrate that the pRb binding domain of T-ag is required for induction of Purkinje cell loss. These findings indicate that a pRb function is required well beyond the completion of Purkinje neuron differentiation and provide a link between cell cycle regulation and neurodegeneration in vivo.
Assuntos
Genes do Retinoblastoma/fisiologia , Células de Purkinje/fisiologia , Animais , Antígenos Transformantes de Poliomavirus/genética , Apoptose/genética , Sequência de Bases , Northern Blotting , Ciclo Celular/genética , Divisão Celular/fisiologia , Sobrevivência Celular/genética , Cerebelo/patologia , DNA/biossíntese , Dano ao DNA , Imuno-Histoquímica , Camundongos , Camundongos Transgênicos , Dados de Sequência Molecular , Células de Purkinje/citologiaRESUMO
SV40 T antigen (Tag) expression directed to cerebellar Purkinje cells resulted in the generation of three transgenic mouse lines that displayed ataxia, a neurological phenotype characteristic of cerebellar dysfunction. Onset of symptoms and cerebellar pathology, characterized by specific Purkinje cell degeneration, appeared to be directly dependent upon transgene copy number. The SV5 line (containing > 30 transgene copies), exhibited embryonic transgene expression that caused selective death of immature Purkinje cells and a subsequent block in cerebellar development and ataxia at 2 weeks. The developmental effect of the disruption of Purkinje cells in SV5 mice suggests that a normal complement of these cells is required for early development of the cerebellar cortex, especially granule cell proliferation and migration from external to internal layers. Transgene expression in a second line, SV4 (10 copies), was detectable during the second postnatal week. Death of mature Purkinje cells in the SV4 line resulted in onset of ataxia at 9 weeks. Ataxia in a third line, SV6 (2 copies), was detected after 15 weeks. The distinct cerebellar phenotypes of the SV4-6 lines correlate with specific Tag-induced Purkinje cell ablation as opposed to tumorigenesis.