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1.
Sci Rep ; 10(1): 19331, 2020 11 09.
Artigo em Inglês | MEDLINE | ID: mdl-33168883

RESUMO

Bioinspired smart materials represent a tremendously growing research field and the obtainment of new building blocks is at the molecular basis of this technology progress. In this work, colloidal materials have been prepared in few steps starting from ribonucleosides. Nucleobase morpholino ß-amino acids are the chimera key intermediates allowing Phe-Phe dipeptides' functionalization with adenine and thymine. The obtained compounds self-aggregate showing enhanced photoluminescent features, such as deep blue fluorescence and phosphorescence emissions.

2.
J Periodontal Res ; 55(4): 503-510, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32096230

RESUMO

OBJECTIVES: The aim of this study was to investigate whether a peptide-based coating can prevent the adhesion of Porphyromonas gingivalis, a key human pathogen associated with periodontitis and peri-implantitis. BACKGROUND: Nonsurgical and surgical interventions have been used for the treatment of peri-implantitis; however, the effectiveness of these approaches is usually unsatisfactory. The main reason is that dental plaque on the surface of the implant is difficult to remove due to its rough surface and thread design. Recently, a peptide-based coating for implant surfaces that can reject the adhesion of Escherichia coli and improve the attachment of host cells was developed. METHODS: A salivary pellicle was created on the surfaces of peptide-coated bare discs and verified with anti-human immunoglobulin G, A and M, and anti-fibrinogen. Early colonizers, Veillonella parvula and Streptococcus sobrinus, and the later colonizer, Porphyromonas gingivalis, were labelled with green and red fluorescent dyes, respectively, and seeded on the discs. Bacterial attachment was semi-quantified by fluorescence intensity. RESULTS: The salivary pellicle was evenly distributed on the discs, with or without the peptide coating, with an average thickness of 3.84 µm. A multi-species dental biofilm was created on the salivary pellicle. The peptide coating resulted in an approximate 25% reduction in the attachment of Veillonella parvula and Streptococcus sobrinus, and a 50% reduction in Porphyromonas gingivalis, when compared to control, uncoated implant discs. CONCLUSION: The novel peptide-based coating can inhibit the attachment of Porphyromonas gingivalis. It may have the potential to impede the development of peri-implantitis.


Assuntos
Implantes Dentários , Peri-Implantite , Porphyromonas gingivalis , Biofilmes , Implantes Dentários/microbiologia , Humanos , Porphyromonas gingivalis/isolamento & purificação , Veillonella
3.
ACS Appl Mater Interfaces ; 11(1): 1201-1208, 2019 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-30565453

RESUMO

Polyelectrolyte multilayers (PEMs) assembled layer-by-layer have emerged as functional polymer films that are both stable and capable of containing drug molecules for controlled release applications. Most of these applications concentrate on sustained release, where the concentration of the released molecules remains rather constant with time. However, high-efficiency delivery requires obtaining high local concentrations at the vicinity of the cells, which is achieved by triggered release. Here, we show that a nanopatterned PEM platform demonstrates superior properties with respect to drug retention and triggered delivery. A chemically modified block copolymer film was used as a template for the selective deposition of poly(ethylene imine) and a charged derivative of the electroactive poly(3,4-ethylenedioxythiophene) together with a drug molecule. This nanopatterned PEM shows the following advantages: (1) high drug loading; (2) enhanced retention of the bioactive molecule; (3) release triggered by an electrochemical stimulus; (4) high efficacy of drug delivery to cells adsorbed on the surface compared to the delivery efficacy of a similar concentration of drug to cells suspended in a solution.


Assuntos
Sistemas de Liberação de Medicamentos/métodos , Técnicas Eletroquímicas/métodos , Iminas , Membranas Artificiais , Polietilenos , Animais , Iminas/química , Iminas/farmacologia , Camundongos , Células NIH 3T3 , Polietilenos/química , Polietilenos/farmacologia
4.
Adv Mater ; 30(41): e1707083, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-29989255

RESUMO

Noncovalent interactions are the main driving force in the folding of proteins into a 3D functional structure. Motivated by the wish to reveal the mechanisms of the associated self-assembly processes, scientists are focusing on studying self-assembly processes of short protein segments (peptides). While this research has led to major advances in the understanding of biological and pathological process, only in recent years has the applicative potential of the resulting self-assembled peptide assemblies started to be explored. Here, major advances in the development of biomimetic supramolecular peptide assemblies as coatings, gels, and as electroactive materials, are highlighted. The guiding lines for the design of helical peptides, ß strand peptides, as well as surface binding monolayer-forming peptides that can be utilized for a specific function are highlighted. Examples of their applications in diverse immerging applications in, e.g., ecology, biomedicine, and electronics, are described. Taking into account that, in addition to extraordinary design flexibility, these materials are naturally biocompatible and ecologically friendly, and their production is cost effective, the emergence of devices incorporating these biomimetic materials in the market is envisioned in the near future.


Assuntos
Nanoestruturas/química , Peptídeos/química , Peptídeos/síntese química , Animais , Materiais Biocompatíveis/síntese química , Materiais Biocompatíveis/química , Equipamentos e Provisões Elétricas , Humanos , Hidrogéis/síntese química , Hidrogéis/química
5.
ACS Biomater Sci Eng ; 4(12): 4051-4061, 2018 Dec 10.
Artigo em Inglês | MEDLINE | ID: mdl-33418805

RESUMO

Due to extension of life expectancy, millions of people suffer nowadays from bone and dental malfunctions that can only be treated by different types of implants. However, these implants tend to fail due to bacterial infection and lack of integration with the remaining tissue. Here, we demonstrate a new concept in which we use specifically designed peptides, in a "Lego-like" manner to endow multiple preprogrammed functions. We developed a bifunctional peptide-based coating that simultaneously rejects the adhesion of infecting bacteria and attracts cells that build the new connecting tissue. The peptide design contains fluorinated phenylalanine that mediates the self-assembly of the peptide into a coating that resists bacterial adhesion. It also includes an Arg-Gly-Asp (RGD) motif that attracts mammalian cells. The whole compound is attached to the surface using a third unit, the amino acid 3,4-dihydroxyphenylalanine (DOPA). This novel, yet very simple approach is significantly advantageous for practical use and synthesis. More importantly, this peptide design can serve as a general platform for generating functional coatings.

6.
Org Lett ; 17(18): 4468-71, 2015 Sep 18.
Artigo em Inglês | MEDLINE | ID: mdl-26335611

RESUMO

The synthesis and the structural characterization of dipeptides composed of unnatural fluorine-substituted ß(2,3)-diarylamino acid and L-alanine are reported. Depending on the stereochemistry of the ß amino acid, these dipeptides are able to self-assemble into proteolytic stable nanotubes. These architectures were able to enter the cell and locate in the cytoplasmic/perinuclear region and represent interesting candidates for biomedical applications.


Assuntos
Alanina/química , Aminoácidos/química , Dipeptídeos/síntese química , Flúor/química , Cristalografia por Raios X , Dipeptídeos/química , Dipeptídeos/farmacologia , Conformação Molecular , Estrutura Molecular , Nanotubos , Ressonância Magnética Nuclear Biomolecular , Estereoisomerismo
7.
Chem Commun (Camb) ; 51(25): 5432-5, 2015 Mar 28.
Artigo em Inglês | MEDLINE | ID: mdl-25470201

RESUMO

This communication describes the co-assembly of polydopamine spheres, either bare or coated with Fe3O4 magnetic nanoparticles, with the short aromatic peptide diphenylalanine. The combination of polydopamine particles and diphenylalanine generated tubular structures decorated with adhesive spherical particles, while the co-assembly of the polydopamine spheres coated with magnetic Fe3O4 nanoparticles with diphenylalanine resulted in the formation of a magnetic hydrogel. These new architectures may be useful as new vehicles for several applications including tissue regeneration and drug delivery.


Assuntos
Hidrogéis/síntese química , Indóis/síntese química , Nanopartículas de Magnetita/química , Peptídeos/química , Fenilalanina/análogos & derivados , Polímeros/síntese química , Dipeptídeos , Hidrogéis/química , Indóis/química , Fenômenos Magnéticos , Tamanho da Partícula , Fenilalanina/química , Polímeros/química , Propriedades de Superfície
8.
J Pept Sci ; 20(7): 479-86, 2014 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-24889029

RESUMO

Functional structures and materials are formed spontaneously in nature through the process of self-assembly. Mimicking this process in vitro will lead to the formation of new substances that would impact many areas including energy production and storage, biomaterials and implants, and drug delivery. The considerable structural diversity of peptides makes them appealing building blocks for self-assembly in vitro. This paper describes the self-assembly of three aromatic dipeptides containing an azide moiety: H-Phe(4-azido)-Phe(4-azido)-OH, H-Phe(4-azido)-Phe-OH, and H-Phe-Phe(4-azido)-OH. The peptide H-Phe(4-azido)-Phe(4-azido)-OH self-assembled into porous spherical structures, whereas the peptides H-Phe(4-azido)-Phe-OH and H-Phe-Phe(4-azido)-OH did not form any ordered structures under the examined experimental conditions. The azido group of the peptide can serve as a photo cross-linking agent upon irradiation with UV light. To examine the effect of this group and its activity on the self-assembled structures, we irradiated the assemblies in solution for different time periods. Using electron microscopy, we determined that the porous spherical assemblies formed by the peptide H-Phe(4-azido)-Phe(4-azido)-OH underwent a structural change upon irradiation. In addition, using FT-IR, we detected the chemical change of the peptide azido group. Moreover, using indentation experiments with atomic force microscopy, we showed that the Young's modulus of the spherical assemblies increased after 20 min of irradiation with UV light. Overall, irradiating the solution of the peptide assemblies containing the azido group resulted in a change both in the morphology and mechanical properties of the peptide-based structures. These ordered assemblies or their peptide monomer building blocks can potentially be incorporated into other peptide assemblies to generate stiffer and more stable materials.


Assuntos
Azidas/química , Dipeptídeos/química , Nanopartículas/química , Reagentes de Ligações Cruzadas/química , Módulo de Elasticidade , Microscopia de Força Atômica , Microscopia Eletrônica de Varredura , Conformação Molecular , Nanopartículas/ultraestrutura , Polimerização , Espectroscopia de Infravermelho com Transformada de Fourier
9.
J Vis Exp ; (81): e50946, 2013 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-24301009

RESUMO

In nature, complex functional structures are formed by the self-assembly of biomolecules under mild conditions. Understanding the forces that control self-assembly and mimicking this process in vitro will bring about major advances in the areas of materials science and nanotechnology. Among the available biological building blocks, peptides have several advantages as they present substantial diversity, their synthesis in large scale is straightforward, and they can easily be modified with biological and chemical entities(1,2). Several classes of designed peptides such as cyclic peptides, amphiphile peptides and peptide-conjugates self-assemble into ordered structures in solution. Homoaromatic dipeptides, are a class of short self-assembled peptides that contain all the molecular information needed to form ordered structures such as nanotubes, spheres and fibrils(3-8). A large variety of these peptides is commercially available. This paper presents a procedure that leads to the formation of ordered structures by the self-assembly of homoaromatic peptides. The protocol requires only commercial reagents and basic laboratory equipment. In addition, the paper describes some of the methods available for the characterization of peptide-based assemblies. These methods include electron and atomic force microscopy and Fourier-Transform Infrared Spectroscopy (FT-IR). Moreover, the manuscript demonstrates the blending of peptides (coassembly) and the formation of a "beads on a string"-like structure by this process.(9) The protocols presented here can be adapted to other classes of peptides or biological building blocks and can potentially lead to the discovery of new peptide-based structures and to better control of their assembly.


Assuntos
Oligopeptídeos/química , Dipeptídeos/química , Ésteres do Ácido Fórmico/química , Microscopia de Força Atômica , Microscopia Eletrônica de Varredura , Nanotubos/química , Estrutura Secundária de Proteína , Espectroscopia de Infravermelho com Transformada de Fourier
10.
ACS Nano ; 6(11): 9559-66, 2012 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-23061818

RESUMO

This paper describes the formation of complex peptide-based structures by the coassembly of two simple peptides, the diphenylalanine peptide and its tert-butyl dicarbonate (Boc) protected analogue. Each of these peptides can self-assemble into a distinct architecture: the diphenylalanine peptide into tubular structures and its analogue into spheres. Integrated together, these peptides coassemble into a construction of beaded strings, where spherical assemblies are connected by elongated elements. Electron and scanning force microscopy demonstrated the morphology of these structures, which we termed "biomolecular necklaces". Additional experiments indicated the reversibility of the coassembly process and the stability of the structures. Furthermore, we suggest a possible mechanism of formation for the biomolecular necklaces. Our suggestion is based on the necklace model for polyelectrolyte chains, which proposes that a necklace structure appears as a result of counterion condensation on the backbone of a polyelectrolyte. Overall, the approach of coassembly, demonstrated using aromatic peptides, can be adapted to any peptides and may lead to the development and discovery of new self-assembled architectures formed by peptides and other biomolecules.


Assuntos
Biopolímeros/química , Cristalização/métodos , Dipeptídeos/química , Hidrocarbonetos Aromáticos/química , Teste de Materiais , Tamanho da Partícula
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